Tris-Cl

Tris-HCl or tris(hydroxymethyl)amino methane, is a solution frequently used in biochemistry made from Tris base and concentrated hydrochloric acid (HCl_aq). To make 1 mol/L Tris-HCl dissolve 121.1 g of tris base in 700 ml of double distilled water, bring to desired pH with concentrated HCl_aq (usually 7.5 or 8.0) but can also have a pH of 8.8, add double distilled water to 1 L, filter with 0.5 μm filter, autoclave, and store at room temperature.