STX8
From Wikipedia, the free encyclopedia
| Syntaxin 8 | |||||||||||||
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 PDB rendering based on 1gl2. | |||||||||||||
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| Identifiers | |||||||||||||
| Symbols | STX8; CARB | ||||||||||||
| External IDs | OMIM: 604203 MGI: 1890156 HomoloGene: 37973 GeneCards: STX8 Gene | ||||||||||||
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| RNA expression pattern | |||||||||||||
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| Orthologs | |||||||||||||
| Species | Human | Mouse | |||||||||||
| Entrez | 9482 | 55943 | |||||||||||
| Ensembl | ENSG00000170310 | ENSMUSG00000020903 | |||||||||||
| UniProt | Q9UNK0 | O88983 | |||||||||||
| RefSeq (mRNA) | NM_004853 | NM_018768 | |||||||||||
| RefSeq (protein) | NP_004844 | NP_061238 | |||||||||||
| Location (UCSC) | Chr 17: 9.15 – 9.48 Mb | Chr 11: 67.97 – 68.21 Mb | |||||||||||
| PubMed search | |||||||||||||
Syntaxin-8 is a protein that in humans is encoded by the STX8 gene.[1][2][3] Syntaxin 8 directly interacts with HECTd3 and has similar subcellular localization.[4] The protein has been shown to form the SNARE complex with syntaxin 7, vti1b and endobrevin. These function as the machinery for the homotypic fusion of late endosomes.[5]
Model organisms
| Characteristic | Phenotype |
|---|---|
| Homozygote viability | Normal |
| Body weight | Normal |
| Anxiety | Normal |
| Neurological assessment | Normal |
| Grip strength | Normal |
| Hot plate | Normal |
| Dysmorphology | Normal |
| Indirect calorimetry | Normal |
| Glucose tolerance test | Normal |
| Auditory brainstem response | Normal |
| DEXA | Normal |
| Radiography | Normal |
| Body temperature | Normal |
| Eye morphology | Normal |
| Clinical chemistry | Normal |
| Haematology | Normal |
| Peripheral blood lymphocytes | Normal |
| Micronucleus test | Normal |
| Heart weight | Normal |
| Skin Histopathology | Normal |
| Brain histopathology | Normal |
| Eye Histopathology | Normal |
| Salmonella infection | Normal[6] |
| Citrobacter infection | Normal[7] |
| All tests and analysis from[8][9] |
Model organisms have been used in the study of STX8 function. A conditional knockout mouse line, called Stx8tm2a(EUCOMM)Wtsi[10][11] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.[12][13][14] Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[8][15] Twenty four tests were carried out on homozygous mutant adult mice, however no significant abnormalities were observed.[8]
Interactions
STX8 has been shown to interact with Vesicle-associated membrane protein 8,[16] VTI1B[17][18] and STX7.[16]
References
- ↑ Steegmaier M, Yang B, Yoo JS, Huang B, Shen M, Yu S, Luo Y, Scheller RH (January 1999). "Three novel proteins of the syntaxin/SNAP-25 family". J Biol Chem 273 (51): 34171–9. doi:10.1074/jbc.273.51.34171. PMID 9852078.
- ↑ Thoreau V, Berges T, Callebaut I, Guillier-Gencik Z, Gressin L, Bernheim A, Karst F, Mornon JP, Kitzis A, Chomel JC (May 1999). "Molecular cloning, expression analysis, and chromosomal localization of human syntaxin 8 (STX8)". Biochem Biophys Res Commun 257 (2): 577–83. doi:10.1006/bbrc.1999.0503. PMID 10198254.
- ↑ "Entrez Gene: STX8 syntaxin 8".
- ↑ Zhang L, Kang L, Bond W, Zhang N (February 2009). "Interaction between syntaxin 8 and HECTd3, a HECT domain ligase". Cell Mol Neurobiol 29 (1): 115–21. doi:10.1007/s10571-008-9303-0. PMID 18821010.
- ↑ Kasai K, Suga K, Izumi T, Akagawa K (October 2008). "Syntaxin 8 has two functionally distinct di-leucine-based motifs". Cell Mol Bio Lett 13 (1): 144–54. doi:10.2478/s11658-007-0043-9. PMID 17965969.
- ↑ "Salmonella infection data for Stx8". Wellcome Trust Sanger Institute.
- ↑ "Citrobacter infection data for Stx8". Wellcome Trust Sanger Institute.
- ↑ 8.0 8.1 8.2 Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x.
- ↑ Mouse Resources Portal, Wellcome Trust Sanger Institute.
- ↑ "International Knockout Mouse Consortium".
- ↑ "Mouse Genome Informatics".
- ↑ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
- ↑ Dolgin E (June 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
- ↑ Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
- ↑ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
- ↑ 16.0 16.1 Antonin, W; Holroyd C, Fasshauer D, Pabst S, Von Mollard G F, Jahn R (December 2000). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". EMBO J. (ENGLAND) 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. ISSN 0261-4189. PMC 305878. PMID 11101518.
- ↑ Rual, Jean-François; Venkatesan Kavitha, Hao Tong, Hirozane-Kishikawa Tomoko, Dricot Amélie, Li Ning, Berriz Gabriel F, Gibbons Francis D, Dreze Matija, Ayivi-Guedehoussou Nono, Klitgord Niels, Simon Christophe, Boxem Mike, Milstein Stuart, Rosenberg Jennifer, Goldberg Debra S, Zhang Lan V, Wong Sharyl L, Franklin Giovanni, Li Siming, Albala Joanna S, Lim Janghoo, Fraughton Carlene, Llamosas Estelle, Cevik Sebiha, Bex Camille, Lamesch Philippe, Sikorski Robert S, Vandenhaute Jean, Zoghbi Huda Y, Smolyar Alex, Bosak Stephanie, Sequerra Reynaldo, Doucette-Stamm Lynn, Cusick Michael E, Hill David E, Roth Frederick P, Vidal Marc (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature (England) 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
- ↑ Subramaniam, V N; Loh E, Horstmann H, Habermann A, Xu Y, Coe J, Griffiths G, Hong W (March 2000). "Preferential association of syntaxin 8 with the early endosome". J. Cell. Sci. (ENGLAND) 113 (6): 997–1008. ISSN 0021-9533. PMID 10683148.
Further reading
- Prekeris R, Yang B, Oorschot V, et al. (1999). "Differential roles of syntaxin 7 and syntaxin 8 in endosomal trafficking". Mol. Biol. Cell 10 (11): 3891–908. PMC 25687. PMID 10564279.
- Subramaniam VN, Loh E, Horstmann H, et al. (2000). "Preferential association of syntaxin 8 with the early endosome". J. Cell. Sci. 113 (6): 997–1008. PMID 10683148.
- McShea A, Samuel T, Eppel JT, et al. (2000). "Identification of CIP-1-associated regulator of cyclin B (CARB), a novel p21-binding protein acting in the G2 phase of the cell cycle". J. Biol. Chem. 275 (30): 23181–6. doi:10.1074/jbc.M001772200. PMID 10781590.
- Scales SJ, Chen YA, Yoo BY, et al. (2000). "SNAREs contribute to the specificity of membrane fusion". Neuron 26 (2): 457–64. doi:10.1016/S0896-6273(00)81177-0. PMID 10839363.
- Antonin W, Holroyd C, Fasshauer D, et al. (2001). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". EMBO J. 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. PMC 305878. PMID 11101518.
- Wade N, Bryant NJ, Connolly LM, et al. (2001). "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells". J. Biol. Chem. 276 (23): 19820–7. doi:10.1074/jbc.M010838200. PMID 11278762.
- Antonin W, Fasshauer D, Becker S, et al. (2002). "Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs". Nat. Struct. Biol. 9 (2): 107–11. doi:10.1038/nsb746. PMID 11786915.
- Bogdanovic A, Bennett N, Kieffer S, et al. (2002). "Syntaxin 7, syntaxin 8, Vti1 and VAMP7 (vesicle-associated membrane protein 7) form an active SNARE complex for early macropinocytic compartment fusion in Dictyostelium discoideum". Biochem. J. 368 (Pt 1): 29–39. doi:10.1042/BJ20020845. PMC 1222979. PMID 12175335.
- Xu Y, Martin S, James DE, Hong W (2003). "GS15 forms a SNARE complex with syntaxin 5, GS28, and Ykt6 and is implicated in traffic in the early cisternae of the Golgi apparatus". Mol. Biol. Cell 13 (10): 3493–507. doi:10.1091/mbc.E02-01-0004. PMC 129961. PMID 12388752.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Kim BY, Ueda M, Kominami E, et al. (2004). "Identification of mouse Vps16 and biochemical characterization of mammalian class C Vps complex". Biochem. Biophys. Res. Commun. 311 (3): 577–82. doi:10.1016/j.bbrc.2003.10.030. PMID 14623309.
- Zhu X, McShea A, Harris PL, et al. (2004). "Elevated expression of a regulator of the G2/M phase of the cell cycle, neuronal CIP-1-associated regulator of cyclin B, in Alzheimer's disease". J. Neurosci. Res. 75 (5): 698–703. doi:10.1002/jnr.20028. PMID 14991845.
- Bilan F, Thoreau V, Nacfer M, et al. (2005). "Syntaxin 8 impairs trafficking of cystic fibrosis transmembrane conductance regulator (CFTR) and inhibits its channel activity". J. Cell. Sci. 117 (Pt 10): 1923–35. doi:10.1242/jcs.01070. PMID 15039462.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Barrios-Rodiles M, Brown KR, Ozdamar B, et al. (2005). "High-throughput mapping of a dynamic signaling network in mammalian cells". Science 307 (5715): 1621–5. doi:10.1126/science.1105776. PMID 15761153.
- Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
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