RHOBTB3
From Wikipedia, the free encyclopedia
| Rho-related BTB domain containing 3 | |||||||||||||
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| Identifiers | |||||||||||||
| Symbols | RHOBTB3; KIAA0878 | ||||||||||||
| External IDs | OMIM: 607353 MGI: 1920546 HomoloGene: 8932 GeneCards: RHOBTB3 Gene | ||||||||||||
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| RNA expression pattern | |||||||||||||
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| More reference expression data | |||||||||||||
| Orthologs | |||||||||||||
| Species | Human | Mouse | |||||||||||
| Entrez | 22836 | 73296 | |||||||||||
| Ensembl | ENSG00000164292 | ENSMUSG00000021589 | |||||||||||
| UniProt | O94955 | Q9CTN4 | |||||||||||
| RefSeq (mRNA) | NM_014899 | NM_028493 | |||||||||||
| RefSeq (protein) | NP_055714 | NP_082769 | |||||||||||
| Location (UCSC) | Chr 5: 95.05 – 95.16 Mb | Chr 13: 75.87 – 75.94 Mb | |||||||||||
| PubMed search | |||||||||||||
Rho-related BTB domain-containing protein 3 is a protein that in humans is encoded by the RHOBTB3 gene.[1][2][3]
RHOBTB3 is a member of the evolutionarily conserved RhoBTB subfamily of Rho GTPases. For background information on RHOBTBs, see RHOBTB1 (MIM 607351).[supplied by OMIM][3]
Model organisms
| Characteristic | Phenotype |
|---|---|
| Homozygote viability | Normal |
| Fertility | Normal |
| Body weight | Abnormal[4] |
| Anxiety | Normal |
| Neurological assessment | Normal |
| Grip strength | Abnormal[5] |
| Hot plate | Normal |
| Dysmorphology | Normal |
| Indirect calorimetry | Normal |
| Glucose tolerance test | Normal |
| Auditory brainstem response | Normal |
| DEXA | Abnormal[6] |
| Radiography | Abnormal[7] |
| Body temperature | Normal |
| Eye morphology | Normal |
| Clinical chemistry | Normal |
| Haematology | Normal |
| Peripheral blood lymphocytes | Normal |
| Micronucleus test | Normal |
| Heart weight | Normal |
| Skin Histopathology | Normal |
| Brain histopathology | Normal |
| Citrobacter infection | Normal[8] |
| All tests and analysis from[9][10] |
Model organisms have been used in the study of RHOBTB3 function. A conditional knockout mouse line, called Rhobtb3tm1a(KOMP)Wtsi[11][12] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[13][14][15]
Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[9][16] Twenty three tests were carried out on mutant mice and four significant abnormalities were observed.[9] Homozygote mutant males had a decreased body weight and abnormal tooth morphology; females had decreased forepaw grip strength and both sexes had a decreased body length.[9]
References
- ↑ Rivero F, Dislich H, Glockner G, Noegel AA (Mar 2001). "The Dictyostelium discoideum family of Rho-related proteins". Nucleic Acids Res 29 (5): 1068–79. doi:10.1093/nar/29.5.1068. PMC 29714. PMID 11222756.
- ↑ Boureux A, Vignal E, Faure S, Fort P (Dec 2006). "Evolution of the Rho family of ras-like GTPases in eukaryotes". Mol Biol Evol 24 (1): 203–16. doi:10.1093/molbev/msl145. PMC 2665304. PMID 17035353.
- ↑ 3.0 3.1 "Entrez Gene: RHOBTB3 Rho-related BTB domain containing 3".
- ↑ "Body weight data for Rhobtb3". Wellcome Trust Sanger Institute.
- ↑ "Grip strength data for Rhobtb3". Wellcome Trust Sanger Institute.
- ↑ "DEXA data for Rhobtb3". Wellcome Trust Sanger Institute.
- ↑ "Radiography data for Rhobtb3". Wellcome Trust Sanger Institute.
- ↑ "Citrobacter infection data for Rhobtb3". Wellcome Trust Sanger Institute.
- ↑ 9.0 9.1 9.2 9.3 Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
- ↑ Mouse Resources Portal, Wellcome Trust Sanger Institute.
- ↑ "International Knockout Mouse Consortium".
- ↑ "Mouse Genome Informatics".
- ↑ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
- ↑ Dolgin E (2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
- ↑ Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
- ↑ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
Further reading
- Andersson B, Wentland MA, Ricafrente JY et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
- Yu W, Andersson B, Worley KC et al. (1997). "Large-scale concatenation cDNA sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
- Nagase T, Ishikawa K, Suyama M et al. (1999). "Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 5 (6): 355–64. doi:10.1093/dnares/5.6.355. PMID 10048485.
- Ramos S, Khademi F, Somesh BP, Rivero F (2003). "Genomic organization and expression profile of the small GTPases of the RhoBTB family in human and mouse". Gene 298 (2): 147–57. doi:10.1016/S0378-1119(02)00980-0. PMID 12426103.
- Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Ota T, Suzuki Y, Nishikawa T et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Brandenberger R, Wei H, Zhang S et al. (2005). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nat. Biotechnol. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197.
- Gerhard DS, Wagner L, Feingold EA et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
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