PstI

From Wikipedia, the free encyclopedia

PstI, is a Type II restriction endonuclease (or restriction enzyme) from Providencia stuartii.[1] PstI recognition and cut site are as follows:

Recognition SequenceCut Site
 5'CTGCAG 3'
 3'GACGTC 5'
 
 5'--CTGCA  G--3'
 3'--G  ACGTC—5'

References

  1. ↑ "Thermo Scientific Restriction Enzymes: PstI". Thermo Scientific. 
3.1.1: Carboxylic ester hydrolases
3.1.2: Thioesterase
3.1.3: Phosphatase
3.1.4: Phosphodiesterase
3.1.6: Sulfatase
Nuclease (includes
deoxyribonuclease and
ribonuclease)
3.1.11-16: Exonuclease
Exodeoxyribonuclease
Exoribonuclease
3.1.21-31: Endonuclease
Endodeoxyribonuclease
Endoribonuclease
either deoxy- or ribo-
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