Peridinin

Peridinin
IUPAC name Acetic acid [(1S,3R)-3-hydroxy-4-[(3E,5E,7E,9E,11Z)-11-[4-[(E)-2-[(1S,4S,6R)-4-hydroxy-2,2,6-trimethyl-7-oxabicyclo[4.1.0]heptan-1-yl]vinyl]-5-oxo-2-furylidene]-3,10-dimethylundeca-1,3,5,7,9-pentaenylidene]-3,5,5-trimethylcyclohexyl] ester
Identifiers
CAS number	33281-81-1 Y
PubChem	5289155
	Jmol-3D images	{{#if:[H][C@@](/[C@@](C)=C/C=C/C=C/C=C(C)/C=C2C=C(/C=C/[C@@]3(O4)[C@]4(C)C[C@@H](O)CC(C)3C)C(O\2)=O)=C=C1[C@](C)(O)C[C@@H](OC(C)=O)CC(C)1C|Image 1
SMILES [H][C@@](/[C@@](C)=C/C=C/C=C/C=C(C)/C=C2C=C(/C=C/[C@@]3(O4)[C@]4(C)C[C@@H](O)CC(C)3C)C(O\2)=O)=C=C1[C@](C)(O)C[C@@H](OC(C)=O)CC(C)1C
Properties
Molecular formula	C39H50O7
Molar mass	630.81 g mol−1
Y (verify) (what is: Y/N?) Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
Infobox references

Peridinin is a light-harvesting carotenoid, a pigment associated with chlorophyll and found in the peridinin-chlorophyll-protein (PCP) light-harvesting complex in dinoflagellates, best studied in Amphidinium carterae.^[1]

Structural characteristics

The PCP complex is unique in its high ratio of peridinin to chlorophyll; most light-harvesting complexes contain more chlorophyll than carotenoids, but the PCP contains eight peridinin and two chlorophyll molecules arranged to promote peridinin-chlorophyll energy transfer. The PCP complex is a protein trimer with an unusual alpha solenoid protein fold with a molecular weight of 35kDa.

The peridinin-chlorophyll-protein light-harvesting complex. The peridinin molecules are pink and the chlorophyll molecules are black.

Spectral characteristics

Emission and excitation spectra of Peridinin Chlorophyll (PerCP)

• Absorption maximum: 483 nm<
• Emission maximum: 676 nm

• Extinction coefficient (ε): 1.96 x 10⁶ M^-1cm^-1

• A₄₈₃/A₂₈₀ ≥ 4.6

Applications

Peridinin chlorophyll (PerCP) is commonly used in immunoassays such as fluorescence-activated cell sorting (FACS) and flow cytometry. The fluorophore is covalently linked to proteins or antibodies for use in research applications.^[2]

References