PFKL

Phosphofructokinase, liver
Identifiers
Symbols	PFKL; PFK-B
External IDs	OMIM: 171860 MGI: 97547 HomoloGene: 55668 ChEMBL: 2191 GeneCards: PFKL Gene
EC number	2.7.1.11
Gene Ontology Molecular function • 6-phosphofructokinase activity • protein binding • ATP binding • kinase binding • identical protein binding • metal ion binding • fructose binding • fructose-6-phosphate binding Cellular component • cytosol • 6-phosphofructokinase complex Biological process • carbohydrate metabolic process • fructose 6-phosphate metabolic process • glucose metabolic process • glycolysis • response to glucose stimulus • fructose 1,6-bisphosphate metabolic process • small molecule metabolic process • negative regulation of insulin secretion • protein oligomerization • protein homotetramerization Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	5211	18641
Ensembl	ENSG00000141959	ENSMUSG00000020277
UniProt	P17858	P12382
RefSeq (mRNA)	NM_001002021	NM_008826
RefSeq (protein)	NP_002617	NP_032852
Location (UCSC)	Chr 21: 45.72 – 45.75 Mb	Chr 10: 77.99 – 78.01 Mb
PubMed search
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6-phosphofructokinase, liver type is an enzyme that in humans is encoded by the PFKL gene.^[1] Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Two transcript variants encoding different isoforms have been found for this gene.^[1]

Interactive pathway map

Click on genes, proteins and metabolites below to link to respective articles. ^{[§ 1]}

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Glycolysis and Gluconeogenesis edit

1. ↑ The interactive pathway map can be edited at WikiPathways: "GlycolysisGluconeogenesis_WP534". 

Model organisms

Model organisms have been used in the study of PFKL function. A conditional knockout mouse line, called Pfkl^{tm1a(EUCOMM)Wtsi[6][7]} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.^[8][9][10]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.^[4][11] Twenty six tests were carried out on mutant mice and three significant abnormalities were observed.^[4] Few homozygous mutant embryos were identified during gestation, and none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice and a hair follicle degeneration phenotype was observed.^[4]

References

Further reading