Nutrient agar
Nutrient agar is a microbiological growth medium commonly used for the routine cultivation of non-fastidious bacteria. It is useful because it remains solid even at relatively high temperatures. Also, bacteria grown in nutrient agar grows on the surface, and is clearly visible as small colonies. In nutrient broth, the bacteria grows in the liquid, and is seen as a soupy substance, not as clearly distinguishable clumps. Nutrient agar typically contains (mass/volume):[1]
- 0.5% Peptone
- 0.3% beef extract/yeast extract
- 1.5% agar
- 0.5% NaCl
- distilled water
- pH adjusted to neutral (6.8) at 25 °C.
See also
References
- ↑ American Public Health Association, American Chemical Society, Association of Official Agricultural Chemists (1920). Standard Methods for the Examination of Water and Sewage. American public health association. p. 95.
2. Lapage S., Shelton J. and Mitchell T., 1970, Methods in Microbiology', Norris J. and Ribbons D., (Eds.), Vol. 3A, Academic Press, London.
3. MacFaddin J. F., 2000, Biochemical Tests for Identification of Medical Bacteria, 3rd Ed., Lippincott, Williams and Wilkins, Baltimore.
4. Downes F. P. and Ito K., (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., American Public Health Association, Washington, D.C.
5. American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., Washington D.C.
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