Naringinase
Naringinase is a debittering enzyme that is used in the commercial production of grapefruit juice. It breaks down the compound naringin that gives grapefruit juics its bitter taste. It is a multienzyme complex which possesses alpha-L-rhamnosidase and beta glucosidase active centers. The E.C. No.(EC 3.2.1.40) of the naringinase and rhamnosidase are the same. First rhamnosidase breaks naringin into prunin and rhamnose. Lastly glucosidase breaks prunin into glucose and naringenin, a flavorless flavanone also found in grapefruit.
Ram gene is a rare gene; it is found in very few microorganisms, like some Bacillus species. It is mainly hydrolysed by the Aspergillus niger, but production of naringinase from fungus is a difficult task as the growth rate of fungi is much slower than that of bacteria.[1][2][3][4]
References
- ↑ Puri, Munish; Kalra, Sukirti (2005). "Purification and characterization of naringinase from a newly isolated strain of Aspergillus niger 1344 for the transformation of flavonoids". World Journal of Microbiology and Biotechnology 21 (5): 753. doi:10.1007/s11274-004-5488-7.
- ↑ Studies on the applicability of alginateentrapped naringinase for the debittering of kinnow juice, Elsevier
- ↑ Puri, M; Banerjee, UC (2000). "Production, purification, and characterization of the debittering enzyme naringinase". Biotechnology Advances 18 (3): 207–17. doi:10.1016/S0734-9750(00)00034-3. PMID 14538108.
- ↑ Puri, Munish; Banerjee, Anirban; Banerjee, U.C. (2005). "Optimization of process parameters for the production of naringinase by Aspergillus niger MTCC 1344". Process Biochemistry 40: 195. doi:10.1016/j.procbio.2003.12.009.