MOPS

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This article is about the biochemical buffering agent. For information about other types of mops, see Mops (disambiguation).
MOPS
IUPAC name

3-morpholinopropane-1-sulfonic acid

Other names

3-(N-morpholino)propanesulfonic acid

Identifiers
CAS number 1132-61-2 YesY
PubChem 2723950
ChemSpider 63972 N
Jmol-3D images Image 1
Properties
Molecular formula C7H15NO4S
Molar mass 209.2633
 N (verify) (what is: YesY/N?)
Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
Infobox references

MOPS is the common name for the compound 3-(N-morpholino)propanesulfonic acid, a buffer introduced by Good et al. in the 1960s. It is a structural analog to MES.[1] Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.

Applications

MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis.[2] Usage above 20 mM in mammalian cell culture work is not recommended.[3]

See also

References

  1. ↑ Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. (1966). "Hydrogen Ion Buffers for Biological Research". Biochemistry 5 (2): 467–77. doi:10.1021/bi00866a011. PMID 5942950. 
  2. ↑ Thomas, J; Hodes, ME (1981). "A new discontinuous buffer system for the electrophoresis of cationic proteins at near-neutral pH". Analytical Biochemistry 118 (1): 194–6. doi:10.1016/0003-2697(81)90178-0. PMID 6278979. 
  3. ↑ Eagle, H. (1971). "Buffer Combinations for Mammalian Cell Culture". Science 174 (4008): 500–3. doi:10.1126/science.174.4008.500. PMID 5110427. 

External links

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