Indirect immunoperoxidase assay

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Microtiter plates with 96, 384 and 1536 wells

Indirect immunoperoxidase assay (IPA) is a laboratory technique used to detect and tirate viruses that do not cause measurable cytopathic effects and cannot be measured by classical plaque assays. These viruses include human coronavirus 229E and OC43.[1]

Methodology

Susceptible cells are inoculated with serial logarithmic dilutions of samples in a 96-well plate. After viral growth, viral detection by IPA yields the infectious virus titer, expressed as “tissue culture infectious dose” (TCID50). This represents the dilution of a virus-containing sample at which half of a series of laboratory wells contain replicating virus. This technique is a reliable method for the titration of human coronaviruses (HCoV) in biological samples (cells, tissues, or fluids). It is also reliable in the detection of antibodies to Human cytomegalovirus.[2][3]

See also

External links

References

  1. ↑ Francine Lambert, Hélène Jacomy, Gabriel Marceau1, Pierre J Talbot. Titration of Human Coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay. SARS- and Other Coronaviruses : Laboratory Protocols Series: Methods in Molecular Biology. Volume: 454. Pub. Date: May-01-2008. 93-102. DOI: 10.1007/978-1-59745-181-9_8
  2. ↑ Francine Lambert, Hélène Jacomy, Gabriel Marceau1, Pierre J Talbot. Titration of Human Coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay. SARS- and Other Coronaviruses : Laboratory Protocols Series: Methods in Molecular Biology. Volume: 454. Pub. Date: May-01-2008. 93-102. DOI: 10.1007/978-1-59745-181-9_8
  3. ↑ G Gerna, C J McCloud and R W Chambers: Immunoperoxidase technique for detection of antibodies to Human cytomegalovirus. J. Clin. Microbiol. 1976, 3(3):364.
Medical test: Immunologic techniques and tests (CPT 86000–86849)
Immunologic techniques
and tests
Other
Inflammation

M: LMC

cell/phys/auag/auab/comp, igrc

imdf/ipig/hyps/tumr

proc, drug (L3/4)

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