IMP dehydrogenase

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IMP dehydrogenase

Structure of IMPDH[1]
Identifiers
EC number 1.1.1.205
CAS number 9028-93-7
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO

IMP dehydrogenase EC 1.1.1.205 (Inosine-5'-monophosphate dehydrogenase) (Inosinic acid dehydrogenaseis) (IMPDH) an enzyme that converts inosine monophosphate to xanthosine monophosphate:[2][3][4][5]

inosine 5'-phosphate + NAD+ + H2O \rightleftharpoons xanthosine 5'-phosphate + NADH + H+

It catalyzes the rate-limiting reaction of de novo GTP biosynthesis.[6]

IMP dehydrogenase is associated with cell proliferation and is a possible target for cancer chemotherapy. Mammalian and bacterial IMPDHs are tetramers of identical chains. There are two IMP dehydrogenase isozymes in humans.[7] IMP dehydrogenase nearly always contains a long insertion that has two CBS domains within it.

The structure of this enzyme is composed of a TIM barrel domain with two CBS domains inserted within a loop.[1][4]

It is inhibited by Mycophenolic acid ribavirin, and 6TGMP (6-thioguanine monophosphate). 6TGMP inhibition prevents purine interconversion and thus the synthesis of purine nucleotides.

Examples

Humans express the following two IMP dehydrogenase isozymes:

IMP dehydrogenase 1
Identifiers
Symbol IMPDH1
Alt. symbols RP10
Entrez 3614
HUGO 6052
OMIM 146690
RefSeq NM_000883
UniProt P20839
Other data
Locus Chr. 7 q31.3-q32
IMP dehydrogenase 2
Identifiers
Symbol IMPDH2
Alt. symbols IMPD2
Entrez 3615
HUGO 6053
OMIM 146691
RefSeq NM_000884
UniProt P12268
Other data
Locus Chr. 3 p21.2

See also

References

  1. ↑ 1.0 1.1 Prosise GL, Luecke H (February 2003). "Crystal structures of Tritrichomonasfoetus inosine monophosphate dehydrogenase in complex with substrate, cofactor and analogs: a structural basis for the random-in ordered-out kinetic mechanism". J. Mol. Biol. 326 (2): 517–27. doi:10.1016/S0022-2836(02)01383-9. PMID 12559919. 
  2. ↑ Magasanik B, Moyed HS, Gehring LB (May 1957). "Enzymes essential for the biosynthesis of nucleic acid guanine; inosine 5'-phosphate dehydrogenase of Aerobacter aerogenes". J. Biol. Chem. 226 (1): 339–50. PMID 13428767. 
  3. ↑ Turner JF, King JE (April 1961). "Inosine 5'-phosphate dehydrogenase of pea seeds". Biochem. J. 79: 147–51. PMC 1205560. PMID 13778733. 
  4. ↑ 4.0 4.1 Hedstrom L (July 2009). "IMP dehydrogenase: structure, mechanism, and inhibition". Chem. Rev. 109 (7): 2903–28. doi:10.1021/cr900021w. PMC 2737513. PMID 19480389. 
  5. ↑ Pimkin M, Markham GD (2009). "Inosine 5'-monophosphate dehydrogenase". Adv. Enzymol. Relat. Areas Mol. Biol. 76: 1–53. PMID 18990827. 
  6. ↑ Collart FR, Huberman E (October 1988). "Cloning and sequence analysis of the human and Chinese hamster inosine-5'-monophosphate dehydrogenase cDNAs". J. Biol. Chem. 263 (30): 15769–72. PMID 2902093. 
  7. ↑ Natsumeda Y, Ohno S, Kawasaki H, Konno Y, Weber G, Suzuki K (March 1990). "Two distinct cDNAs for human IMP dehydrogenase". J. Biol. Chem. 265 (9): 5292–5. PMID 1969416. 

Further reading

  • Wang J, Yang JW, Zeevi A, Webber SA, Girnita DM, Selby R, Fu J, Shah T, Pravica V, Hutchinson IV, Burckart GJ (May 2008). "IMPDH1 gene polymorphisms and association with acute rejection in renal transplant patients". Clin. Pharmacol. Ther. 83 (5): 711–7. doi:10.1038/sj.clpt.6100347. PMID 17851563. 
Oxidoreductases: alcohol oxidoreductases (EC 1.1)
1.1.1: NAD/NADP acceptor
1.1.2: cytochrome acceptor
1.1.3: oxygen acceptor
1.1.4: disulfide as acceptor
1.1.5: quinone/similar acceptor
1.1.99: other acceptors
  • B
  • enzm
  • 1.1
    • 2
    • 3
    • 4
    • 5
    • 6
    • 7
    • 8
    • 10
    • 11
    • 13
    • 14
    • 15-18
  • 2.1
    • 2
    • 3
    • 4
    • 5
    • 6
    • 7
      • 2.7.10
      • 11-12
    • 8
  • 3.1
      • 3.1.3.48
    • 2
    • 3
    • 4
      • 3.4.21
      • 22
      • 23
      • 24
    • 5
    • 6
    • 7
  • 4.1
    • 2
    • 3
    • 4
    • 5
    • 6
  • 5.1
    • 2
    • 3
    • 4
    • 99
  • 6.1-3
    • 4
    • 5-6
Purine metabolism
Anabolism
R5P→IMP:
IMP→AMP:
IMP→GMP:
Nucleotide salvage
Catabolism
Pyrimidine metabolism
Anabolism
Catabolism
Deoxyribonucleotides

M: MET

mt, k, c/g/r/p/y/i, f/h/s/l/o/e, a/u, n, m

k, cgrp/y/i, f/h/s/l/o/e, au, n, m, epon

m (A16/C10), i (k, c/g/r/p/y/i, f/h/s/o/e, a/u, n, m)

This article incorporates text from the public domain Pfam and InterPro IPR001093


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