Higher-energy collisional dissociation

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"HCD" redirects here. For the Host Controller Driver, see Host controller interface. For HCD Research, see MediaCurves. For the Swiss ice hockey club, see HC Davos. For the California state agency, see California Department of Housing and Community Development.

Higher-energy collisional dissociation (HCD, formerly higher-energy C-trap dissociation) is a fragmentation technique.^[1] Frequently, this type of dissociation is used in conjunction with the orbitrap mass analyzer. In this arrangement, the ions pass through the C-trap and into the HCD cell, an added octopole collision cell, where dissociation takes place. The ions are then returned to the C-trap before injection into the orbitrap for mass analysis. It does not suffer from the low mass cutoff of resonant-excitation collision-induced dissociation (CID), and therefore is useful for isobaric tag–based quantification as reporter ions can be observed.

References

↑ Olsen JV, Macek B, Lange O, Makarov A, Horning S, Mann M (September 2007). "Higher-energy C-trap dissociation for peptide modification analysis". Nat. Methods 4 (9): 709–12. doi:10.1038/nmeth1060. PMID 17721543.

v t e Mass spectrometry

Mass m/z Mass spectrum MS software Acronyms

Ion source	APCI APLI CI DAPPI DART DESI DIOS EESI EI ESI FAB FD GD IA ICP LAESI MALDI MIP SS SSI TI TS

Mass analyzer	Sector Wien filter Time-of-flight Quadrupole mass filter Quadrupole ion trap FT-ICR Orbitrap

Detector	Electron multiplier Microchannel plate detector Daly detector Faraday cup Langmuir–Taylor detector

MS combination	MS/MS QqQ Hybrid MS GC/MS LC/MS IMS/MS

Fragmentation	BIRD CID ECD EDD ETD HCD IRMPD NETD SID

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