GNAI1

From Wikipedia, the free encyclopedia

Guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1

PDB rendering based on 1agr.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
1KJY, 1Y3A, 2G83, 2GTP, 2IK8, 2OM2, 2XNS, 3ONW, 3QE0, 3QI2, 3UMR, 3UMS, 4G5Q

Identifiers

Symbols

GNAI1; Gi

External IDs

OMIM: 139310 MGI: 95771 HomoloGene: 74417 ChEMBL: 4741 GeneCards: GNAI1 Gene

Gene Ontology
Molecular function	• GTPase activity • signal transducer activity • protein binding • GTP binding • G-protein beta/gamma-subunit complex binding • G-protein coupled serotonin receptor binding • metal ion binding
Cellular component	• nucleus • cytoplasm • centrosome • heterotrimeric G-protein complex • plasma membrane • midbody • extrinsic to internal side of plasma membrane
Biological process	• cell cycle • G-protein coupled receptor signaling pathway • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway • synaptic transmission • blood coagulation • platelet activation • response to peptide hormone stimulus • cell division
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 7:
79.76 – 79.85 Mb

Chr 5:
18.27 – 18.36 Mb

PubMed search

Guanine nucleotide-binding protein G(i), alpha-1 subunit is a protein that in humans is encoded by the GNAI1 gene.^[1]^[2]

Interactive pathway map

Click on genes, proteins and metabolites below to link to respective articles. ^{[§ 1]}

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Nicotine Activity on Dopaminergic Neurons edit

↑ The interactive pathway map can be edited at WikiPathways: "NicotineDopaminergic_WP1602".

Interactions

GNAI1 has been shown to interact with GPR143,^[3] RGS14,^[4]^[5] RIC8A,^[6] S1PR1,^[7] RGS12^[4] and RGS19.^[8]^[9]

References

↑ Bray P, Carter A, Guo V, Puckett C, Kamholz J, Spiegel A, Nirenberg M (August 1987). "Human cDNA clones for an alpha subunit of Gi signal-transduction protein". Proc Natl Acad Sci U S A 84 (15): 5115–9. doi:10.1073/pnas.84.15.5115. PMC 298804. PMID 3110783.
↑ "Entrez Gene: GNAI1 Guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1".
↑ Schiaffino, M V; d'Addio M, Alloni A, Baschirotto C, Valetti C, Cortese K, Puri C, Bassi M T, Colla C, De Luca M, Tacchetti C, Ballabio A (September 1999). "Ocular albinism: evidence for a defect in an intracellular signal transduction system". Nat. Genet. (UNITED STATES) 23 (1): 108–12. doi:10.1038/12715. ISSN 1061-4036. PMID 10471510. Cite uses deprecated parameters (help)
↑ 4.0 4.1 Kimple, R J; De Vries L, Tronchère H, Behe C I, Morris R A, Gist Farquhar M, Siderovski D P (August 2001). "RGS12 and RGS14 GoLoco motifs are G alpha(i) interaction sites with guanine nucleotide dissociation inhibitor Activity". J. Biol. Chem. (United States) 276 (31): 29275–81. doi:10.1074/jbc.M103208200. ISSN 0021-9258. PMID 11387333. Cite uses deprecated parameters (help)
↑ Kimple, Randall J; Kimple Michelle E, Betts Laurie, Sondek John, Siderovski David P (April 2002). "Structural determinants for GoLoco-induced inhibition of nucleotide release by Galpha subunits". Nature (England) 416 (6883): 878–81. doi:10.1038/416878a. ISSN 0028-0836. PMID 11976690. Cite uses deprecated parameters (help)
↑ Tall, Gregory G; Krumins Andrejs M, Gilman Alfred G (March 2003). "Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein guanine nucleotide exchange factor". J. Biol. Chem. (United States) 278 (10): 8356–62. doi:10.1074/jbc.M211862200. ISSN 0021-9258. PMID 12509430. Cite uses deprecated parameters (help)
↑ Lee, M J; Evans M, Hla T (May 1996). "The inducible G protein-coupled receptor edg-1 signals via the G(i)/mitogen-activated protein kinase pathway". J. Biol. Chem. (UNITED STATES) 271 (19): 11272–9. doi:10.1074/jbc.271.19.11272. ISSN 0021-9258. PMID 8626678. Cite uses deprecated parameters (help)
↑ Woulfe, D S; Stadel J M (June 1999). "Structural basis for the selectivity of the RGS protein, GAIP, for Galphai family members. Identification of a single amino acid determinant for selective interaction of Galphai subunits with GAIP". J. Biol. Chem. (UNITED STATES) 274 (25): 17718–24. doi:10.1074/jbc.274.25.17718. ISSN 0021-9258. PMID 10364213. Cite uses deprecated parameters (help)
↑ De Vries, L; Elenko E, Hubler L, Jones T L, Farquhar M G (December 1996). "GAIP is membrane-anchored by palmitoylation and interacts with the activated (GTP-bound) form of Gαi subunits". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 93 (26): 15203–8. doi:10.1073/pnas.93.26.15203. ISSN 0027-8424. PMC 26381. PMID 8986788. Cite uses deprecated parameters (help)

Sidhu A, Niznik HB (2000). "Coupling of dopamine receptor subtypes to multiple and diverse G proteins". Int. J. Dev. Neurosci. 18 (7): 669–77. doi:10.1016/S0736-5748(00)00033-2. PMID 10978845.
Brown EJ, Frazier WA (2001). "Integrin-associated protein (CD47) and its ligands". Trends Cell Biol. 11 (3): 130–5. doi:10.1016/S0962-8924(00)01906-1. PMID 11306274.
Raymond JR, Mukhin YV, Gelasco A et al. (2002). "Multiplicity of mechanisms of serotonin receptor signal transduction". Pharmacol. Ther. 92 (2–3): 179–212. doi:10.1016/S0163-7258(01)00169-3. PMID 11916537.
Jiang M, Pandey S, Tran VT, Fong HK (1991). "Guanine nucleotide-binding regulatory proteins in retinal pigment epithelial cells". Proc. Natl. Acad. Sci. U.S.A. 88 (9): 3907–11. doi:10.1073/pnas.88.9.3907. PMC 51562. PMID 1902575.
Gennity JM, Siess W (1991). "Thrombin inhibits the pertussis-toxin-dependent ADP-ribosylation of a novel soluble Gi-protein in human platelets". Biochem. J. 279 (Pt 3): 643–50. PMC 1151493. PMID 1953657.
Itoh H, Toyama R, Kozasa T et al. (1988). "Presence of three distinct molecular species of Gi protein alpha subunit. Structure of rat cDNAs and human genomic DNAs". J. Biol. Chem. 263 (14): 6656–64. PMID 2834384.
Bloch DB, Bloch KD, Iannuzzi M et al. (1988). "The gene for the alpha i1 subunit of human guanine nucleotide binding protein maps near the cystic fibrosis locus". Am. J. Hum. Genet. 42 (6): 884–8. PMC 1715192. PMID 3130752.
Nagata K, Katada T, Tohkin M et al. (1988). "GTP-binding proteins in human platelet membranes serving as the specific substrate of islet-activating protein, pertussis toxin". FEBS Lett. 237 (1–2): 113–7. doi:10.1016/0014-5793(88)80182-0. PMID 3139448.
Kagimoto S, Yamada Y, Kubota A et al. (1994). "Human somatostatin receptor, SSTR2, is coupled to adenylyl cyclase in the presence of Gi alpha 1 protein". Biochem. Biophys. Res. Commun. 202 (2): 1188–95. doi:10.1006/bbrc.1994.2054. PMID 7914078.
Nitta K, Uchida K, Kawashima A et al. (1994). "Identification of GTP-binding proteins in human glomeruli". Nippon Jinzo Gakkai shi 36 (1): 9–12. PMID 8107314.
Law SF, Zaina S, Sweet R et al. (1994). "Gi alpha 1 selectively couples somatostatin receptor subtype 3 to adenylyl cyclase: identification of the functional domains of this alpha subunit necessary for mediating the inhibition by somatostatin of cAMP formation". Mol. Pharmacol. 45 (4): 587–90. PMID 8183236.
Europe-Finner GN, Phaneuf S, Watson SP, López Bernal A (1993). "Identification and expression of G-proteins in human myometrium: up-regulation of G alpha s in pregnancy". Endocrinology 132 (6): 2484–90. doi:10.1210/en.132.6.2484. PMID 8504751.
Laugwitz KL, Allgeier A, Offermanns S et al. (1996). "The human thyrotropin receptor: a heptahelical receptor capable of stimulating members of all four G protein families". Proc. Natl. Acad. Sci. U.S.A. 93 (1): 116–20. doi:10.1073/pnas.93.1.116. PMC 40189. PMID 8552586.
Andersson B, Wentland MA, Ricafrente JY et al. (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
Lee MJ, Evans M, Hla T (1996). "The inducible G protein-coupled receptor edg-1 signals via the G(i)/mitogen-activated protein kinase pathway". J. Biol. Chem. 271 (19): 11272–9. doi:10.1074/jbc.271.19.11272. PMID 8626678.
De Vries L, Elenko E, Hubler L et al. (1997). "GAIP is membrane-anchored by palmitoylation and interacts with the activated (GTP-bound) form of Gαi subunits". Proc. Natl. Acad. Sci. U.S.A. 93 (26): 15203–8. doi:10.1073/pnas.93.26.15203. PMC 26381. PMID 8986788.
Yu W, Andersson B, Worley KC et al. (1997). "Large-Scale Concatenation cDNA Sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
Popov S, Yu K, Kozasa T, Wilkie TM (1997). "The regulators of G protein signaling (RGS) domains of RGS4, RGS10, and GAIP retain GTPase activating protein activity in vitro". Proc. Natl. Acad. Sci. U.S.A. 94 (14): 7216–20. doi:10.1073/pnas.94.14.7216. PMC 23796. PMID 9207071.
Shuey DJ, Betty M, Jones PG et al. (1998). "RGS7 attenuates signal transduction through the G(alpha q) family of heterotrimeric G proteins in mammalian cells". J. Neurochem. 70 (5): 1964–72. doi:10.1046/j.1471-4159.1998.70051964.x. PMID 9572280.

PDB gallery

1agr: COMPLEX OF ALF4-ACTIVATED GI-ALPHA-1 WITH RGS4

1as0: GTP-GAMMA-S BOUND G42V GIA1

1as2: GDP+PI BOUND G42V GIA1

1as3: GDP BOUND G42V GIA1

1bh2: A326S MUTANT OF AN INHIBITORY ALPHA SUBUNIT

1bof: GI-ALPHA-1 BOUND TO GDP AND MAGNESIUM

1cip: GI-ALPHA-1 SUBUNIT OF GUANINE NUCLEOTIDE-BINDING PROTEIN COMPLEXED WITH A GTP ANALOGUE

1gdd: TERTIARY AND QUATERNARY STRUCTURAL CHANGES IN GIA1 INDUCED BY GTP HYDROLYSIS

1gfi: STRUCTURES OF ACTIVE CONFORMATIONS OF GI ALPHA 1 AND THE MECHANISM OF GTP HYDROLYSIS

1gg2: G PROTEIN HETEROTRIMER MUTANT GI_ALPHA_1(G203A) BETA_1 GAMMA_2 WITH GDP BOUND

1gia: STRUCTURE OF ACTIVE CONFORMATIONS OF GIA1 AND THE MECHANISM OF GTP HYDROLYSIS

1gil: STRUCTURE OF ACTIVE CONFORMATIONS OF GIA1 AND THE MECHANISM OF GTP HYDROLYSIS

1git: STRUCTURE OF GTP-BINDING PROTEIN

1gp2: G PROTEIN HETEROTRIMER GI_ALPHA_1 BETA_1 GAMMA_2 WITH GDP BOUND

1kjy: Crystal Structure of Human G[alpha]i1 Bound to the GoLoco Motif of RGS14

1svk: Structure of the K180P mutant of Gi alpha subunit bound to AlF4 and GDP

1svs: Structure of the K180P mutant of Gi alpha subunit bound to GppNHp.

1y3a: Structure of G-Alpha-I1 bound to a GDP-selective peptide provides insight into guanine nucleotide exchange

2g83: Structure of activated G-alpha-i1 bound to a nucleotide-state-selective peptide: Minimal determinants for recognizing the active form of a G protein alpha subunit

2gtp: Crystal structure of the heterodimeric complex of human RGS1 and activated Gi alpha 1

2hlb: A Structural Basis for Nucleotide Exchange on G-alpha-i Subunits and Receptor Coupling Specificity

2ihb: Crystal structure of the heterodimeric complex of human RGS10 and activated Gi alpha 3

2ik8: Crystal structure of the heterodimeric complex of human RGS16 and activated Gi alpha 1

2ode: Crystal structure of the heterodimeric complex of human RGS8 and activated Gi alpha 3

Hydrolases: acid anhydride hydrolases (EC 3.6)

3.6.1

3.6.2

3.6.3-4: ATPase

3.6.3

Cu++ (3.6.3.4)	Menkes/ATP7A Wilson/ATP7B

Ca+ (3.6.3.8)	SERCA ATP2A1 ATP2A2 ATP2A3 Plasma membrane ATP2B1 ATP2B2 ATP2B3 ATP2B4 SPCA ATP2C1 ATP2C2

Na+/K+ (3.6.3.9)	ATP1A1 ATP1A2 ATP1A3 ATP1A4 ATP1B1 ATP1B2 ATP1B3 ATP1B4

H+/K+ (3.6.3.10)	ATP4A

Other P-type ATPase	ATP8B1 ATP10A ATP11B ATP12A ATP13A2 ATP13A3

3.6.4

3.6.5: GTPase

3.6.5.1: Heterotrimeric G protein	G_αs G_αi GNAI1 GNAI2 GNAI3 G_αq/11 GNAQ GNA11 G_α12/13 GNA12 GNA13 Transducin GNAT1 GNAT2

3.6.5.2: Small GTPase > Ras superfamily	Rho family of GTPases: Cdc42 CDC42 TC10 TCL RhoUV RhoU RhoV Rac Rac1 2 3 RhoG RhoBTB 1 2 RhoH Rho A B C Rnd 1 2 3 RhoDF RhoF RhoD other: Ras HRAS KRAS NRAS Rab RAB23 RAB27 Arf ARF6 SAR1B ARL13B ARL6 Ran Rheb Rap RGK

3.6.5.3: Protein-synthesizing GTPase	Prokaryotic IF-2 EF-Tu EF-G Eukaryotic

3.6.5.5-6: Polymerization motors	Dynamin Tubulin

B
enzm
1.1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 10
- 11
- 13
- 14
- 15-18
2.1
- 2
- 3
- 4
- 5
- 6
- 7
  - 2.7.10
  - 11-12
- 8
3.1
- - 3.1.3.48
- 2
- 3
- 4
  - 3.4.21
  - 22
  - 23
  - 24
- 5
- 6
- 7
4.1
- 2
- 3
- 4
- 5
- 6
5.1
- 2
- 3
- 4
- 99
6.1-3
- 4
- 5-6

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GNAI1

Interactive pathway map

Interactions

References

Further reading