EPS15

From Wikipedia, the free encyclopedia
Epidermal growth factor receptor pathway substrate 15

PDB rendering based on 1c07.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
SymbolsEPS15; AF-1P; AF1P; MLLT5
External IDsOMIM: 600051 MGI: 104583 HomoloGene: 128359 GeneCards: EPS15 Gene
RNA expression pattern
More reference expression data
Orthologs
SpeciesHumanMouse
Entrez206013858
EnsemblENSG00000085832ENSMUSG00000028552
UniProtP42566P42567
RefSeq (mRNA)NM_001159969NM_001159964
RefSeq (protein)NP_001153441NP_001153436
Location (UCSC)Chr 1:
51.82 – 51.99 Mb
Chr 4:
109.28 – 109.39 Mb
PubMed search

Epidermal growth factor receptor substrate 15 is a protein that in humans is encoded by the EPS15 gene.[1]

This gene encodes a protein that is part of the EGFR pathway. The protein is present at clathrin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.[2]

Model organisms

Model organisms have been used in the study of EPS15 function. A conditional knockout mouse line, called Eps15tm1a(KOMP)Wtsi[7][8] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.[9][10][11]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[5][12] Twenty six tests were carried out on mutant mice and one significant abnormality was observed: homozygous mutant animals had a decreased mean corpuscular hemoglobin concentration.[5]

Interactions

EPS15 has been shown to interact with REPS2,[13] HGS,[14][15] EPN1,[16] CRK[17] and HRB.[18]

References

  1. Wong WT, Kraus MH, Carlomagno F, Zelano A, Druck T, Croce CM, Huebner K, Di Fiore PP (Jun 1994). "The human eps15 gene, encoding a tyrosine kinase substrate, is conserved in evolution and maps to 1p31-p32". Oncogene 9 (6): 1591–7. PMID 8183552. 
  2. "Entrez Gene: EPS15 epidermal growth factor receptor pathway substrate 15". 
  3. "Salmonella infection data for Eps15". Wellcome Trust Sanger Institute. 
  4. "Citrobacter infection data for Eps15". Wellcome Trust Sanger Institute. 
  5. 5.0 5.1 5.2 Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x. 
  6. Mouse Resources Portal, Wellcome Trust Sanger Institute.
  7. "International Knockout Mouse Consortium". 
  8. "Mouse Genome Informatics". 
  9. Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750. 
  10. Dolgin E (June 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718. 
  11. Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. 
  12. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353. 
  13. Nakashima, S; Morinaka K, Koyama S, Ikeda M, Kishida M, Okawa K, Iwamatsu A, Kishida S, Kikuchi A (July 1999). "Small G protein Ral and its downstream molecules regulate endocytosis of EGF and insulin receptors". EMBO J. (ENGLAND) 18 (13): 3629–42. doi:10.1093/emboj/18.13.3629. ISSN 0261-4189. PMC 1171441. PMID 10393179. 
  14. Bean, A J; Davanger S, Chou M F, Gerhardt B, Tsujimoto S, Chang Y (May 2000). "Hrs-2 regulates receptor-mediated endocytosis via interactions with Eps15". J. Biol. Chem. (UNITED STATES) 275 (20): 15271–8. doi:10.1074/jbc.275.20.15271. ISSN 0021-9258. PMID 10809762. 
  15. Bache, Kristi G; Raiborg Camilla, Mehlum Anja, Stenmark Harald (April 2003). "STAM and Hrs are subunits of a multivalent ubiquitin-binding complex on early endosomes". J. Biol. Chem. (United States) 278 (14): 12513–21. doi:10.1074/jbc.M210843200. ISSN 0021-9258. PMID 12551915. 
  16. Chen, H; Fre S, Slepnev V I, Capua M R, Takei K, Butler M H, Di Fiore P P, De Camilli P (August 1998). "Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis". Nature (ENGLAND) 394 (6695): 793–7. doi:10.1038/29555. ISSN 0028-0836. PMID 9723620. 
  17. Schumacher, C; Knudsen B S, Ohuchi T, Di Fiore P P, Glassman R H, Hanafusa H (June 1995). "The SH3 domain of Crk binds specifically to a conserved proline-rich motif in Eps15 and Eps15R". J. Biol. Chem. (UNITED STATES) 270 (25): 15341–7. doi:10.1074/jbc.270.25.15341. ISSN 0021-9258. PMID 7797522. 
  18. Doria, M; Salcini A E, Colombo E, Parslow T G, Pelicci P G, Di Fiore P P (December 1999). "The eps15 homology (EH) domain-based interaction between eps15 and hrb connects the molecular machinery of endocytosis to that of nucleocytosolic transport". J. Cell Biol. (UNITED STATES) 147 (7): 1379–84. doi:10.1083/jcb.147.7.1379. ISSN 0021-9525. PMC 2174238. PMID 10613896. 

Further reading

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