EIF1AX

From Wikipedia, the free encyclopedia

Eukaryotic translation initiation factor 1A, X-linked

PDB rendering based on 1d7q.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
1D7Q, 3ZJY, 4KZY, 4KZZ

Identifiers

Symbols

EIF1AX; EIF1A; EIF1AP1; EIF4C; eIF-1A; eIF-4C

External IDs

OMIM: 300186 MGI: 1913485 HomoloGene: 20364 GeneCards: EIF1AX Gene

Gene Ontology
Molecular function	• translation initiation factor activity • translation factor activity, nucleic acid binding
Cellular component	• cytosol
Biological process	• translation • translational initiation • gene expression • cellular protein metabolic process
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr X:
20.14 – 20.16 Mb

Chr X:
159.37 – 159.39 Mb

PubMed search

Eukaryotic translation initiation factor 1A, X-chromosomal is a protein that in humans is encoded by the EIF1AX gene.^[1]^[2]^[3]

Mammalian 17-kDa eukaryotic initiation factor, eIF1A (formerly designated eIF-4C), is essential for transfer of the initiator Met-tRNAf (as Met-tRNAf·eIF2·GTP ternary complex) to 40 S ribosomal subunits in the absence of mRNA to form the 40 S preinitiation complex (40 S·Met-tRNAf·eIF2·GTP). Furthermore, eIF1A acts catalytically in this reaction to mediate highly efficient transfer of the Met-tRNAf·eIF2·GTP ternary complex to 40 S ribosomal subunits. The 40 S complex formed is free of eIF1A which indicates that its role in 40 S preinitiation complex formation is not to stabilize the binding of Met-tRNAf to 40 S ribosomes. Additionally, the eIF1A-mediated 40 S initiation complex formed in the presence of AUG codon efficiently joins 60 S ribosomal subunits in an eIF5-dependent reaction to form a functional 80 S initiation complex. Though found in some reports, eIF1A probably plays no role either in the subunit joining reaction or in the generation of ribosomal subunits from 80 S ribosomes. The major function of eIF1A is to mediate the transfer of Met-tRNAf to 40 S ribosomal subunits to form the 40 S preinitiation complex.^[4]

This gene encodes an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA.^[3]

Mutations in this gene have been recurrently seen associated to cases of uveal melanoma with disomy 3 (http://www.ncbi.nlm.nih.gov/pubmed/23793026).

Interactions

EIF1AX has been shown to interact with IPO13.^[5]

References

↑ Dever TE, Wei CL, Benkowski LA, Browning K, Merrick WC, Hershey JW (Mar 1994). "Determination of the amino acid sequence of rabbit, human, and wheat germ protein synthesis factor eIF-4C by cloning and chemical sequencing". J Biol Chem 269 (5): 3212–8. PMID 8106356.
↑ Lahn BT, Page DC (Nov 1997). "Functional coherence of the human Y chromosome". Science 278 (5338): 675–80. doi:10.1126/science.278.5338.675. PMID 9381176.
↑ 3.0 3.1 "Entrez Gene: EIF1AX eukaryotic translation initiation factor 1A, X-linked".
↑ Umadas Maitra, Jayanta Chaudhuri; Jayanta Chaudhuri, Kausik Si (21 March 1997). "Function of Eukaryotic Translation Initiation Factor 1A (eIF1A) (Formerly Called eIF-4C) in Initiation of Protein Synthesis". The Journal of BIological Chemistry 272 (12): 7883–7891. doi:10.1074/jbc.272.12.7883. Retrieved 19 February 2012. Cite uses deprecated parameters (help)
↑ Mingot, J M; Kostka S, Kraft R, Hartmann E, Görlich D (Jul 2001). "Importin 13: a novel mediator of nuclear import and export". EMBO J. (England) 20 (14): 3685–94. doi:10.1093/emboj/20.14.3685. ISSN 0261-4189. PMC 125545. PMID 11447110. Cite uses deprecated parameters (help)

Battiste JL, Pestova TV, Hellen CU, Wagner G (2000). "The eIF1A solution structure reveals a large RNA-binding surface important for scanning function.". Mol. Cell 5 (1): 109–19. doi:10.1016/S1097-2765(00)80407-4. PMID 10678173.
Choi SK, Olsen DS, Roll-Mecak A, et al. (2000). "Physical and functional interaction between the eukaryotic orthologs of prokaryotic translation initiation factors IF1 and IF2.". Mol. Cell. Biol. 20 (19): 7183–91. doi:10.1128/MCB.20.19.7183-7191.2000. PMC 86272. PMID 10982835.
Mingot JM, Kostka S, Kraft R, et al. (2001). "Importin 13: a novel mediator of nuclear import and export.". EMBO J. 20 (14): 3685–94. doi:10.1093/emboj/20.14.3685. PMC 125545. PMID 11447110.
Sørensen HP, Hedegaard J, Sperling-Petersen HU, Mortensen KK (2002). "Remarkable conservation of translation initiation factors: IF1/eIF1A and IF2/eIF5B are universally distributed phylogenetic markers.". IUBMB Life 51 (5): 321–7. doi:10.1080/152165401317190842. PMID 11699879.
Bohnsack MT, Regener K, Schwappach B, et al. (2003). "Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm.". EMBO J. 21 (22): 6205–15. doi:10.1093/emboj/cdf613. PMC 137205. PMID 12426392.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Olsen DS, Savner EM, Mathew A, et al. (2003). "Domains of eIF1A that mediate binding to eIF2, eIF3 and eIF5B and promote ternary complex recruitment in vivo.". EMBO J. 22 (2): 193–204. doi:10.1093/emboj/cdg030. PMC 140105. PMID 12514125.
Marintchev A, Kolupaeva VG, Pestova TV, Wagner G (2003). "Mapping the binding interface between human eukaryotic initiation factors 1A and 5B: a new interaction between old partners.". Proc. Natl. Acad. Sci. U.S.A. 100 (4): 1535–40. doi:10.1073/pnas.0437845100. PMC 149867. PMID 12569173.
Agate RJ, Choe M, Arnold AP (2004). "Sex differences in structure and expression of the sex chromosome genes CHD1Z and CHD1W in zebra finches.". Mol. Biol. Evol. 21 (2): 384–96. doi:10.1093/molbev/msh027. PMID 14660691.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Ross MT, Grafham DV, Coffey AJ, et al. (2005). "The DNA sequence of the human X chromosome.". Nature 434 (7031): 325–37. doi:10.1038/nature03440. PMC 2665286. PMID 15772651.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1.". Nature 441 (7091): 315–21. doi:10.1038/nature04727. PMID 16710414.
Kim S, Kim GJ, Miyoshi H, et al. (2007). "Efficiency of the elongation factor-1alpha promoter in mammalian embryonic stem cells using lentiviral gene delivery systems.". Stem Cells Dev. 16 (4): 537–45. doi:10.1089/scd.2006.0088. PMID 17784828.

PDB gallery

1d7q: HUMAN TRANSLATION INITIATION FACTOR EIF1A

Protein biosynthesis: translation (prokaryotic, eukaryotic)

Ribosomal proteins

Initiation factor

Prokaryotic

Archaeal

aIF1
aIF2
aIF5
aIF6

Eukaryotic

eIF1	EIF1AX AY 1B

eIF2	EIF2S1 EIF2S2 EIF2S3 EIF2B1 EIF2B2 EIF2B3 EIF2B4 EIF2B5 EIF-2 kinase

eIF3	EIF3A B C D F G H I J K M S6

eIF4	EIF4A2 A3 B E1 E2 G1 G2 G3 H

eIF5	EIF5A A2 B

eIF6	EIF6

Elongation factor

Prokaryotic	EF-Tu EF-Ts EF-G

Archaeal	aEF-1, aEF-2

Eukaryotic	EEF-1 EEF1A1 EEF1A2 EEF1A3 EEF1B1 EEF1B2 EEF1B3 EEF1B4 EEF1D EEF1E1 EEF1G EEF2

Release factor

Other

Other concepts

see also disorders of translation and posttranslational modification
B bsyn: dna (repl, cycl, reco, repr) · tscr (fact, tcrg, nucl, rnat, rept, ptts) · tltn (risu, pttl, nexn) · dnab, rnab/runp · stru (domn, 1°, 2°, 3°, 4°)

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EIF1AX

Interactions

References

Further reading