EIF1
| Eukaryotic translation initiation factor 1 | |||||||||||||
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 PDB rendering based on 2if1. | |||||||||||||
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| Identifiers | |||||||||||||
| Symbols | EIF1; A121; EIF-1; EIF1A; ISO1; SUI1 | ||||||||||||
| External IDs | MGI: 3643981 HomoloGene: 130538 GeneCards: EIF1 Gene | ||||||||||||
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| Orthologs | |||||||||||||
| Species | Human | Mouse | |||||||||||
| Entrez | 10209 | 432950 | |||||||||||
| Ensembl | ENSG00000173812 | ENSMUSG00000035530 | |||||||||||
| UniProt | P41567 | P48024 | |||||||||||
| RefSeq (mRNA) | NM_005801 | XM_001473255 | |||||||||||
| RefSeq (protein) | NP_005792 | XP_001473305 | |||||||||||
| Location (UCSC) | Chr 17: 39.85 – 39.85 Mb | Chr 11: 100.32 – 100.32 Mb | |||||||||||
| PubMed search | |||||||||||||
Eukaryotic translation initiation factor 1 is a protein that in humans is encoded by the EIF1 gene.[1][2][3]
Mammalian 17-kDa eukaryotic initiation factor, eIF1A (formerly designated eIF-4C), is essential for transfer of the initiator Met-tRNAf (as Met-tRNAf·eIF2·GTP ternary complex) to 40 S ribosomal subunits in the absence of mRNA to form the 40 S preinitiation complex (40 S·Met-tRNAf·eIF2·GTP). Furthermore, eIF1A acts catalytically in this reaction to mediate highly efficient transfer of the Met-tRNAf·eIF2·GTP ternary complex to 40 S ribosomal subunits. The 40 S complex formed is free of eIF1A which indicates that its role in 40 S preinitiation complex formation is not to stabilize the binding of Met-tRNAf to 40 S ribosomes. Additionally, the eIF1A-mediated 40 S initiation complex formed in the presence of AUG codon efficiently joins 60 S ribosomal subunits in an eIF5-dependent reaction to form a functional 80 S initiation complex. Though found in some reports, eIF1A probably plays no role either in the subunit joining reaction or in the generation of ribosomal subunits from 80 S ribosomes. The major function of eIF1A is to mediate the transfer of Met-tRNAf to 40 S ribosomal subunits to form the 40 S preinitiation complex.[4]
References
- ↑ Fields C, Adams MD (Feb 1994). "Expressed sequence tags identify a human isolog of the suil translation initiation factor". Biochem Biophys Res Commun 198 (1): 288–91. doi:10.1006/bbrc.1994.1040. PMID 7904817.
- ↑ Sheikh MS, Fernandez-Salas E, Yu M, Hussain A, Dinman JD, Peltz SW, Huang Y, Fornace AJ Jr (Jul 1999). "Cloning and characterization of a human genotoxic and endoplasmic reticulum stress-inducible cDNA that encodes translation initiation factor 1(eIF1(A121/SUI1))". J Biol Chem 274 (23): 16487–93. doi:10.1074/jbc.274.23.16487. PMID 10347211.
- ↑ "Entrez Gene: EIF1 eukaryotic translation initiation factor 1".
- ↑ Umadas Maitra, Jayanta Chaudhuri; Jayanta Chaudhuri, Kausik Si (21 March 1997). "Function of Eukaryotic Translation Initiation Factor 1A (eIF1A) (Formerly Called eIF-4C) in Initiation of Protein Synthesis". The Journal of BIological Chemistry 272 (12): 7883–7891. doi:10.1074/jbc.272.12.7883. Retrieved 19 February 2012.
External links
- Cap-dependent translation initiation from Nature Reviews Microbiology. A good image and overview of the function of initiation factors
Further reading
- Hillier LD, Lennon G, Becker M, et al. (1997). "Generation and analysis of 280,000 human expressed sequence tags.". Genome Res. 6 (9): 807–28. doi:10.1101/gr.6.9.807. PMID 8889549.
- Lian Z, Pan J, Liu J, et al. (1999). "The translation initiation factor, hu-Sui1 may be a target of hepatitis B X antigen in hepatocarcinogenesis.". Oncogene 18 (9): 1677–87. doi:10.1038/sj.onc.1202470. PMID 10208429.
- Fletcher CM, Pestova TV, Hellen CU, Wagner G (1999). "Structure and interactions of the translation initiation factor eIF1". EMBO J. 18 (9): 2631–7. doi:10.1093/emboj/18.9.2631. PMC 1171342. PMID 10228174.
- Chin LS, Singh SK, Wang Q, Murray SF (2000). "Identification of okadaic-acid-induced genes by mRNA differential display in glioma cells". J. Biomed. Sci. 7 (2): 152–9. doi:10.1007/BF02256622. PMID 10754390.
- Mendell JT, Medghalchi SM, Lake RG, et al. (2000). "Novel Upf2p Orthologues Suggest a Functional Link between Translation Initiation and Nonsense Surveillance Complexes". Mol. Cell. Biol. 20 (23): 8944–57. doi:10.1128/MCB.20.23.8944-8957.2000. PMC 86549. PMID 11073994.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455.
External links
- EIF1 human gene location in the UCSC Genome Browser.
- EIF1 human gene details in the UCSC Genome Browser.
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