Carbylamine reaction
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The Carbylamine reaction is a chemical test for detection of primary amines. In this reaction, the analyte is heated with alcoholic potassium hydroxide and chloroform. If a primary amine is present, the isocyanide is formed.
For example, the reaction with ethylamine:
The reaction with aniline:
The carbylamine test does not give a positive reaction with secondary and tertiary amines.
References
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