Calf-intestinal alkaline phosphatase

From Wikipedia, the free encyclopedia

Calf-intestinal alkaline phosphatase (CIAP/CIP) is a type of alkaline phosphatase that catalyzes the removal of phosphate groups from the 5' end of DNA strands.[1][2] This enzyme is frequently used in DNA sub-cloning, as DNA fragments that lack the 5' phosphate groups cannot ligate. [3] This prevents recircularization of linearized vector DNA and improves the yield of vector containing the appropriate insert.

References

  1. ↑ Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
  2. ↑ Seeburg, P.H. et al. (1977) Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone. Nature 270, 486–94
  3. ↑ Ullrich, A. et al. (1977) Rat insulin genes: Construction of plasmids containing the coding sequences. Science 196, 1313–9.


3.1.1: Carboxylic ester hydrolases
3.1.2: Thioesterase
3.1.3: Phosphatase
3.1.4: Phosphodiesterase
3.1.6: Sulfatase
Nuclease (includes
deoxyribonuclease and
ribonuclease)
3.1.11-16: Exonuclease
Exodeoxyribonuclease
Exoribonuclease
3.1.21-31: Endonuclease
Endodeoxyribonuclease
Endoribonuclease
either deoxy- or ribo-
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