Beta-carotene 15,15'-monooxygenase
| β-carotene 15,15'-monooxygenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.14.99.36 | ||||||||
| CAS number | 37256-60-3 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / EGO | ||||||||
| |||||||||
In enzymology, β-carotene 15,15'-monooxygenase (EC 1.14.99.36) is an enzyme that catalyzes the biological reaction
- β-carotene + O2 → 2retinal
This is a cleavage reaction which cleaves β-carotene, utilizes molecular oxygen, is enhanced by the presence of bile salts and thyroxine, and generates two molecules of retinal. In humans the enzyme is present in small intestine and liver[1]
This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The systematic name of this enzyme class is β-carotene:oxygen 15,15'-oxidoreductase (bond-cleaving). Other names in common use include β-carotene 15,15'-dioxygenase and β-carotene dioxygenase.
In general, carnivores are poor converters of ionone-containing carotenoids, and pure carnivores such as cats and ferrets lack beta-carotene 15,15'-monooxygenase and cannot convert any carotenoids to retinal (resulting in none of the carotenoids being forms of vitamin A for these species). They must have preformed vitamin A in their diet.
References
- Leuenberger MG, Engeloch-Jarret C and Woggon WD (2001). "The reaction mechanism of the enzyme-catalysed central cleavage of beta-carotene to retinal". Angew. Chem. 40: 2614–2616.
- Goodman DS, Huang HS, Kanai M and Shiratori T (1967). "The enzymatic conversion of all-trans beta-carotene into retinal". J. Biol. Chem. 242: 3543–3554.
- Goodman DS, Huang HS, Shiratori T (1966). "Mechanism of the biosynthesis of vitamin A from beta-carotene". J. Biol. Chem. 241 (9): 1929–32. PMID 5946623.