6G-fructosyltransferase

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6G-fructosyltransferase
Identifiers
EC number 2.4.1.243
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

In enzymology, a 6G-fructosyltransferase (EC 2.4.1.243) is an enzyme that catalyzes the chemical reaction

[1-beta-D-fructofuranosyl-(2->1)-]m+1 alpha-D-glucopyranoside + [1-beta-D-fructofuranosyl-(2->1)-]n+1 alpha-D-glucopyranoside \rightleftharpoons [1-beta-D-fructofuranosyl-(2->1)-]m alpha-D-glucopyranoside + [1-beta-D-fructofuranosyl-(2->1)-]n+1 beta-D-fructofuranosyl-(2->6)-alpha-D-glucopyranoside (m > 0; n >\rightleftharpoons 0)

Thus, the two substrates of this enzyme are [[[1-beta-D-fructofuranosyl-(2->1)-]m+1 alpha-D-glucopyranoside]] and [[[1-beta-D-fructofuranosyl-(2->1)-]n+1 alpha-D-glucopyranoside]], whereas its 4 products are [[[1-beta-D-fructofuranosyl-(2->1)-]m alpha-D-glucopyranoside]], [[[1-beta-D-fructofuranosyl-(2->1)-]n+1]], beta-D-fructofuranosyl-(2->6)-alpha-D-glucopyranoside (m > 0; n >=, and 0.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is 1F-oligo[beta-D-fructofuranosyl-(2->1)-]sucrose 6G-beta-D-fructotransferase. Other names in common use include fructan:fructan 6G-fructosyltransferase, 1F(1-beta-D-fructofuranosyl)m, sucrose:1F(1-beta-D-fructofuranosyl)nsucrose, 6G-fructosyltransferase, 6G-FFT, 6G-FT, and 6G-fructotransferase.

References

    • Shiomi N (1981). "Purification and characterisation of 6G-fructosyltransferase from the roots of asparagus (Asparagus officinalis L.)". Carbohydr. Res. 96 (2): 281292. doi:10.1016/S0008-6215(00)81878-9. 
    • Shiomi N (1982). "Reverse reaction of fructosyl transfer catalysed by asparagus 6G-fructosyltransferase". Carbohydr. Res. 106: 166169. doi:10.1016/S0008-6215(00)80743-0. 
    • Shiomi N and Ueno K (2004). "Cloning and expression of genes encoding fructosyltransferases from higher plants in food technology". J. Appl. Glycosci. 51 (2): 177183. doi:10.5458/jag.51.177. 
    • Ueno K, Onodera S, Kawakami A, Yoshida M, Shiomi N (2005). "Molecular characterization and expression of a cDNA encoding fructan:fructan 6G-fructosyltransferase from asparagus (Asparagus officinalis)". New. Phytol. 165 (3): 81324. doi:10.1111/j.1469-8137.2004.01294.x. PMID 15720693. 


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