ZP2

Zona pellucida glycoprotein 2 (sperm receptor)

Identifiers

ZP2; ZPA; Zp-2

External IDs

OMIM: 182888 MGI: 99214 HomoloGene: 48194 GeneCards: ZP2 Gene

Gene Ontology
Molecular function	• protein binding • coreceptor activity • acrosin binding
Cellular component	• extracellular region • proteinaceous extracellular matrix • multivesicular body • endoplasmic reticulum • Golgi apparatus • plasma membrane • integral to membrane • stored secretory granule • extracellular matrix
Biological process	• manganese ion transport • intracellular protein transport • single fertilization • binding of sperm to zona pellucida • regulation of acrosome reaction
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 16:
21.21 – 21.22 Mb

Chr 7:
127.27 – 127.29 Mb

PubMed search

[1]

[2]

Zona pellucida sperm-binding protein 2 is a protein that in humans is encoded by the ZP2 gene.^[1]^[2]

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies.^[2]

References

^ Liang LF, Dean J (Apr 1993). "Conservation of mammalian secondary sperm receptor genes enables the promoter of the human gene to function in mouse oocytes". Dev Biol 156 (2): 399–408. doi:10.1006/dbio.1993.1087. PMID 8385033.
^ ^a ^b "Entrez Gene: ZP2 zona pellucida glycoprotein 2 (sperm receptor)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=7783.

Rankin T, Dean J (2000). "The zona pellucida: using molecular genetics to study the mammalian egg coat". Rev. Reprod. 5 (2): 114–121. doi:10.1530/ror.0.0050114. PMID 10864856.
Mori T, Guo MW, Sato E et al. (2000). "Molecular and immunological approaches to mammalian fertilization". J. Reprod. Immunol. 47 (2): 139–158. doi:10.1016/S0165-0378(00)00055-3. PMID 10924747.
Loftus BJ, Kim UJ, Sneddon VP et al. (1999). "Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q". Genomics 60 (3): 295–308. doi:10.1006/geno.1999.5927. PMID 10493829.
Tsubamoto H, Hasegawa A, Nakata Y et al. (1999). "Expression of recombinant human zona pellucida protein 2 and its binding capacity to spermatozoa". Biol. Reprod. 61 (6): 1649–1654. doi:10.1095/biolreprod61.6.1649. PMID 10570015.
Howes E, Pascall JC, Engel W, Jones R (2002). "Interactions between mouse ZP2 glycoprotein and proacrosin; a mechanism for secondary binding of sperm to the zona pellucida during fertilization". J. Cell. Sci. 114 (Pt 22): 4127–36. PMID 11739644.
Tanii I, Oh-oka T, Yoshinaga K, Toshimori K (2002). "A mouse acrosomal cortical matrix protein, MC41, has ZP2-binding activity and forms a complex with a 75-kDa serine protease". Dev. Biol. 238 (2): 332–341. doi:10.1006/dbio.2001.0380. PMID 11784014.
Kiefer SM, Saling P (2002). "Proteolytic processing of human zona pellucida proteins". Biol. Reprod. 66 (2): 407–414. doi:10.1095/biolreprod66.2.407. PMID 11804956.
Qi H, Williams Z, Wassarman PM (2002). "Secretion and assembly of zona pellucida glycoproteins by growing mouse oocytes microinjected with epitope-tagged cDNAs for mZP2 and mZP3". Mol. Biol. Cell 13 (2): 530–541. doi:10.1091/mbc.01-09-0440. PMC 65647. PMID 11854410. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=65647.
Zhao M, Gold L, Ginsberg AM et al. (2002). "Conserved furin cleavage site not essential for secretion and integration of ZP3 into the extracellular egg coat of transgenic mice". Mol. Cell. Biol. 22 (9): 3111–3120. doi:10.1128/MCB.22.9.3111-3120.2002. PMC 133755. PMID 11940668. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=133755.
Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–16903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Rankin TL, Coleman JS, Epifano O et al. (2003). "Fertility and taxon-specific sperm binding persist after replacement of mouse sperm receptors with human homologs". Dev. Cell 5 (1): 33–43. doi:10.1016/S1534-5807(03)00195-3. PMID 12852850.
Hinsch E, Groeger S, Oehninger S, Hinsch KD (2003). "Localization and functional importance of a conserved zona pellucida 2 protein domain in the human and bovine ovary using monoclonal anti-ZP2 peptide antibodies". Theriogenology 60 (7): 1331–1344. doi:10.1016/S0093-691X(03)00169-9. PMID 14511786.
Gerhard DS, Wagner L, Feingold EA et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–2127. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Furlong LI, Harris JD, Vazquez-Levin MH (2006). "Binding of recombinant human proacrosin/acrosin to zona pellucida (ZP) glycoproteins. I. Studies with recombinant human ZPA, ZPB, and ZPC". Fertil. Steril. 83 (6): 1780–1790. doi:10.1016/j.fertnstert.2004.12.042. PMID 15950651.
Caballero-Campo P, Chirinos M, Fan XJ et al. (2006). "Biological effects of recombinant human zona pellucida proteins on sperm function". Biol. Reprod. 74 (4): 760–768. doi:10.1095/biolreprod.105.047522. PMID 16407501.

ZP2

References

Further reading