USF

USF may refer to:

Universities

• University of South Florida, Tampa, Florida
• University of San Francisco, California
• University of St. Francis, Joliet, Illinois
• University of Sioux Falls, South Dakota

File formats

• Universal Subtitle Format
• Nintendo Ultra 64 Sound Format

Other uses

• Universal Service Fund, a United States government program
• Universal Studios Florida, a theme park
• Upstream stimulatory factor, a DNA-binding protein regulating gene expression