UPF1

UPF1 regulator of nonsense transcripts homolog (yeast)

PDB rendering based on 2gjk.
Identifiers
Symbols UPF1; FLJ43809; FLJ46894; HUPF1; KIAA0221; NORF1; RENT1; pNORF1; smg-2
External IDs OMIM: 601430 MGI: 107995 HomoloGene: 2185 GeneCards: UPF1 Gene
RNA expression pattern
More reference expression data
Orthologs
Species Human Mouse
Entrez 5976 19704
Ensembl ENSG00000005007 ENSMUSG00000058301
UniProt Q92900 Q3UG00
RefSeq (mRNA) NM_002911.3 NM_030680
RefSeq (protein) NP_002902.2 NP_109605
Location (UCSC) Chr 19:
18.94 – 18.98 Mb		 Chr 8:
72.86 – 72.88 Mb
PubMed search [1] [2]

Regulator of nonsense transcripts 1 is a protein that in humans is encoded by the UPF1 gene.[1][2]

This gene encodes a protein that is part of a post-splicing multiprotein complex, the exon junction complex, involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene.[3]

Interactions

UPF1 has been shown to interact with UPF2,[4][5][6][7][8] SMG1,[7] DCP2,[4][9] DCP1A,[9] UPF3A[5][7][8] and UPF3B.[4][5][8]

References

  1. ^ Perlick HA, Medghalchi SM, Spencer FA, Kendzior RJ Jr, Dietz HC (Nov 1996). "Mammalian orthologues of a yeast regulator of nonsense transcript stability". Proc Natl Acad Sci U S A 93 (20): 10928–32. doi:10.1073/pnas.93.20.10928. PMC 38260. PMID 8855285. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=38260. 
  2. ^ Applequist SE, Selg M, Raman C, Jack HM (Mar 1997). "Cloning and characterization of HUPF1, a human homolog of the Saccharomyces cerevisiae nonsense mRNA-reducing UPF1 protein". Nucleic Acids Res 25 (4): 814–21. doi:10.1093/nar/25.4.814. PMC 146496. PMID 9064659. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=146496. 
  3. ^ "Entrez Gene: UPF1 UPF1 regulator of nonsense transcripts homolog (yeast)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5976. 
  4. ^ a b c Lejeune, Fabrice; Li Xiaojie, Maquat Lynne E (Sep. 2003). "Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities". Mol. Cell (United States) 12 (3): 675–87. doi:10.1016/S1097-2765(03)00349-6. ISSN 1097-2765. PMID 14527413. 
  5. ^ a b c Schell, Thomas; Köcher Thomas, Wilm Matthias, Seraphin Bertrand, Kulozik Andreas E, Hentze Matthias W (Aug. 2003). "Complexes between the nonsense-mediated mRNA decay pathway factor human upf1 (up-frameshift protein 1) and essential nonsense-mediated mRNA decay factors in HeLa cells". Biochem. J. (England) 373 (Pt 3): 775–83. doi:10.1042/BJ20021920. ISSN 0264-6021. PMC 1223536. PMID 12723973. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1223536. 
  6. ^ Mendell, J T; Medghalchi S M, Lake R G, Noensie E N, Dietz H C (Dec. 2000). "Novel Upf2p Orthologues Suggest a Functional Link between Translation Initiation and Nonsense Surveillance Complexes". Mol. Cell. Biol. (UNITED STATES) 20 (23): 8944–57. doi:10.1128/MCB.20.23.8944-8957.2000. ISSN 0270-7306. PMC 86549. PMID 11073994. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=86549. 
  7. ^ a b c Yamashita, A; Ohnishi T, Kashima I, Taya Y, Ohno S (Sep. 2001). "Human SMG-1, a novel phosphatidylinositol 3-kinase-related protein kinase, associates with components of the mRNA surveillance complex and is involved in the regulation of nonsense-mediated mRNA decay". Genes Dev. (United States) 15 (17): 2215–28. doi:10.1101/gad.913001. ISSN 0890-9369. PMC 312771. PMID 11544179. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=312771. 
  8. ^ a b c Lykke-Andersen, J; Shu M D, Steitz J A (Dec. 2000). "Human Upf proteins target an mRNA for nonsense-mediated decay when bound downstream of a termination codon". Cell (United States) 103 (7): 1121–31. doi:10.1016/S0092-8674(00)00214-2. ISSN 0092-8674. PMID 11163187. 
  9. ^ a b Lykke-Andersen, Jens (Dec. 2002). "Identification of a Human Decapping Complex Associated with hUpf Proteins in Nonsense-Mediated Decay". Mol. Cell. Biol. (United States) 22 (23): 8114–21. doi:10.1128/MCB.22.23.8114-8121.2002. ISSN 0270-7306. PMC 134073. PMID 12417715. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=134073. 

Further reading