UCHL3

Ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)

PDB rendering based on 1uch.

Available structures
PDB	1UCH, 1XD3

Identifiers

Symbols

UCHL3;

External IDs

OMIM: 603090 MGI: 1355274 HomoloGene: 4377 GeneCards: UCHL3 Gene

EC number

3.4.19.12

Gene Ontology
Molecular function	• ubiquitin thiolesterase activity • protein binding • peptidase activity • cysteine-type peptidase activity • ubiquitin binding
Cellular component	• intracellular • cytoplasm
Biological process	• ubiquitin-dependent protein catabolic process
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 13:
76.12 – 76.18 Mb

Chr 14:
102.05 – 102.1 Mb

PubMed search

[1]

[2]

Ubiquitin carboxyl-terminal hydrolase isozyme L3 is an enzyme that in humans is encoded by the UCHL3 gene.^[1]^[2]

1 Interactions
2 See also
3 References
4 Further reading

Interactions

UCHL3 has been shown to interact with NEDD8 and the tauopathy and synucleinopathy associated mutated ubiquitin molecule UBB+1.^[3]^[4]

References

^ Wilkinson KD, Lee KM, Deshpande S, Duerksen-Hughes P, Boss JM, Pohl J (Dec 1989). "The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase". Science 246 (4930): 670–3. doi:10.1126/science.2530630. PMID 2530630.
^ "Entrez Gene: UCHL3 ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=7347.
^ Dennissen FJ, Kholod N, Hermes DJ, Kemmerling N, Steinbusch HW, Dantuma NP, van Leeuwen FW (July 2011). "Mutant ubiquitin (UBB(+1)) associated with neurodegenerative disorders is hydrolyzed by ubiquitin C-terminal hydrolase L3 (UCH-L3)". FEBS Lett. doi:10.1016/j.febslet.2011.06.037. PMID 21762696.
^ Wada H, Kito K, Caskey LS, Yeh ET, Kamitani T (October 1998). "Cleavage of the C-terminus of NEDD8 by UCH-L3". Biochem. Biophys. Res. Commun. 251 (3): 688–92. doi:10.1006/bbrc.1998.9532. PMID 9790970.

Johnston SC, Larsen CN, Cook WJ, et al. (1997). "Crystal structure of a deubiquitinating enzyme (human UCH-L3) at 1.8 A resolution.". EMBO J. 16 (13): 3787–96. doi:10.1093/emboj/16.13.3787. PMC 1170002. PMID 9233788. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1170002.
Wilkinson KD, Laleli-Sahin E, Urbauer J, et al. (1999). "The binding site for UCH-L3 on ubiquitin: mutagenesis and NMR studies on the complex between ubiquitin and UCH-L3.". J. Mol. Biol. 291 (5): 1067–77. doi:10.1006/jmbi.1999.3038. PMID 10518943.
Baek SH, Yoo YJ, Tanaka K, Chung CH (1999). "Molecular cloning of chick UCH-6 which shares high similarity with human UCH-L3: its unusual substrate specificity and tissue distribution.". Biochem. Biophys. Res. Commun. 264 (1): 235–40. doi:10.1006/bbrc.1999.1492. PMID 10527871.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Saito S, Iida A, Sekine A, et al. (2003). "Catalog of 680 variations among eight cytochrome p450 ( CYP) genes, nine esterase genes, and two other genes in the Japanese population.". J. Hum. Genet. 48 (5): 249–70. doi:10.1007/s10038-003-0021-7. PMID 12721789.
Nam MJ, Madoz-Gurpide J, Wang H, et al. (2004). "Molecular profiling of the immune response in colon cancer using protein microarrays: occurrence of autoantibodies to ubiquitin C-terminal hydrolase L3.". Proteomics 3 (11): 2108–15. doi:10.1002/pmic.200300594. PMID 14595809.
Dunham A, Matthews LH, Burton J, et al. (2004). "The DNA sequence and analysis of human chromosome 13.". Nature 428 (6982): 522–8. doi:10.1038/nature02379. PMC 2665288. PMID 15057823. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2665288.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Misaghi S, Galardy PJ, Meester WJ, et al. (2005). "Structure of the ubiquitin hydrolase UCH-L3 complexed with a suicide substrate.". J. Biol. Chem. 280 (2): 1512–20. doi:10.1074/jbc.M410770200. PMID 15531586.
Rolén U, Kobzeva V, Gasparjan N, et al. (2006). "Activity profiling of deubiquitinating enzymes in cervical carcinoma biopsies and cell lines.". Mol. Carcinog. 45 (4): 260–9. doi:10.1002/mc.20177. PMID 16402389.
Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration.". Cell 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569.

PDB gallery

1uch: DEUBIQUITINATING ENZYME UCH-L3 (HUMAN) AT 1.8 ANGSTROM RESOLUTION

1xd3: Crystal structure of UCHL3-UbVME complex

UCHL3

Contents

Interactions

See also

References

Further reading