TRNT1

TRNA nucleotidyl transferase, CCA-adding, 1

PDB rendering based on 1ou5.

Available structures
PDB	1ou5

Identifiers

Symbols

TRNT1; CCA1; MtCCA

External IDs

OMIM: 612907 MGI: 1917297 HomoloGene: 9333 GeneCards: TRNT1 Gene

EC number

2.7.7.72

Gene Ontology
Molecular function	• tRNA binding • nucleotide binding • tRNA adenylyltransferase activity • ATP binding • transferase activity • nucleotidyltransferase activity
Cellular component	• intracellular • mitochondrion
Biological process	• RNA processing • protein targeting to mitochondrion • tRNA 3'-end processing
Sources: Amigo / QuickGO

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 3:
3.17 – 3.19 Mb

Chr 6:
106.72 – 106.73 Mb

PubMed search

[1]

[2]

tRNA-nucleotidyltransferase 1, mitochondrial is an enzyme that in humans is encoded by the TRNT1 gene.^[1]^[2]^[3] This enzyme adds the nucleotide sequence CCA to the 3' end of tRNA, using ATP and CTP as substrates. The sequence creates the binding site for an amino acid.^[4]

References

^ Lai CH, Chou CY, Ch'ang LY, Liu CS, Lin W (Aug 2000). "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics". Genome Res 10 (5): 703–13. doi:10.1101/gr.10.5.703. PMC 310876. PMID 10810093. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=310876.
^ Nagaike T, Suzuki T, Tomari Y, Takemoto-Hori C, Negayama F, Watanabe K, Ueda T (Oct 2001). "Identification and characterization of mammalian mitochondrial tRNA nucleotidyltransferases". J Biol Chem 276 (43): 40041–9. doi:10.1074/jbc.M106202200. PMID 11504732.
^ "Entrez Gene: TRNT1 tRNA nucleotidyl transferase, CCA-adding, 1". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=51095.
^ Lizano E, Scheibe M, Rammelt C, Betat H, Mörl M (May 2008). "A comparative analysis of CCA-adding enzymes from human and E. coli: differences in CCA addition and tRNA 3'-end repair". Biochimie 90 (5): 762–72. doi:10.1016/j.biochi.2007.12.007. PMID 18226598.

Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=310948.
Wiemann S, Weil B, Wellenreuther R et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=311072.
Simpson JC, Wellenreuther R, Poustka A et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1083732.
Reichert AS, Thurlow DL, Mörl M (2002). "A eubacterial origin for the human tRNA nucleotidyltransferase?". Biol. Chem. 382 (10): 1431–8. doi:10.1515/BC.2001.176. PMID 11727826.
Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Augustin MA, Reichert AS, Betat H et al. (2003). "Crystal structure of the human CCA-adding enzyme: insights into template-independent polymerization". J. Mol. Biol. 328 (5): 985–94. doi:10.1016/S0022-2836(03)00381-4. PMID 12729736.
Gerhard DS, Wagner L, Feingold EA et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Wiemann S, Arlt D, Huber W et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528930.
Kimura K, Wakamatsu A, Suzuki Y et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1356129.
Mehrle A, Rosenfelder H, Schupp I et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1347501.
Lizano E, Schuster J, Müller M et al. (2007). "A splice variant of the human CCA-adding enzyme with modified activity". J. Mol. Biol. 366 (4): 1258–65. doi:10.1016/j.jmb.2006.12.016. PMID 17204286.

PDB gallery

1ou5: Crystal structure of human CCA-adding enzyme

TRNT1

References

Further reading