SRPK1

SRSF protein kinase 1

PDB rendering based on 1wak.

Available structures
PDB	1WAK, 1WBP, 3BEG

Identifiers

Symbols

SRPK1; SFRSK1

External IDs

OMIM: 601939 MGI: 106908 HomoloGene: 110962 GeneCards: SRPK1 Gene

EC number

2.7.11.1

Gene Ontology
Molecular function	• nucleotide binding • magnesium ion binding • protein kinase activity • protein serine/threonine kinase activity • protein binding • ATP binding
Cellular component	• nucleus • cytoplasm
Biological process	• protein phosphorylation • protein phosphorylation • chromosome segregation • intracellular protein kinase cascade • RNA splicing • cell differentiation • interspecies interaction between organisms • positive regulation of viral genome replication • negative regulation of viral genome replication • innate immune response • regulation of mRNA processing
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 6:
35.8 – 35.89 Mb

Chr 17:
28.72 – 28.76 Mb

PubMed search

[1]

[2]

Serine/threonine-protein kinase SRPK1 is an enzyme that in humans is encoded by the SRPK1 gene.^[1]^[2]^[3]

This gene encodes a serine/arginine protein kinase specific for the SR (serine/arginine-rich domain) family of splicing factors. The protein localizes to the nucleus and the cytoplasm. It is thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. A second alternatively spliced transcript variant for this gene has been described, but its full length nature has not been determined.^[3]

Interactions

SRPK1 has been shown to interact with SNRP70^[4]^[5] and ASF/SF2.^[6]^[4]^[7]^[8]

References

^ Gui JF, Lane WS, Fu XD (Jul 1994). "A serine kinase regulates intracellular localization of splicing factors in the cell cycle". Nature 369 (6482): 678–82. doi:10.1038/369678a0. PMID 8208298.
^ Wang HY, Arden KC, Bermingham JR Jr, Viars CS, Lin W, Boyer AD, Fu XD (May 1999). "Localization of serine kinases, SRPK1 (SFRSK1) and SRPK2 (SFRSK2), specific for the SR family of splicing factors in mouse and human chromosomes". Genomics 57 (2): 310–5. doi:10.1006/geno.1999.5770. PMID 10198174.
^ ^a ^b "Entrez Gene: SRPK1 SFRS protein kinase 1". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=6732.
^ ^a ^b Wang, H Y; Lin W, Dyck J A, Yeakley J M, Songyang Z, Cantley L C, Fu X D (Feb. 1998). "SRPK2: A Differentially Expressed SR Protein-specific Kinase Involved in Mediating the Interaction and Localization of Pre-mRNA Splicing Factors in Mammalian Cells". J. Cell Biol. (UNITED STATES) 140 (4): 737–50. doi:10.1083/jcb.140.4.737. ISSN 0021-9525. PMC 2141757. PMID 9472028. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2141757.
^ Kamachi, Makoto; Le Truc M, Kim Susan J, Geiger Meghan E, Anderson Paul, Utz Paul J (Nov. 2002). "Human Autoimmune Sera as Molecular Probes for the Identification of an Autoantigen Kinase Signaling Pathway". J. Exp. Med. (United States) 196 (9): 1213–25. doi:10.1084/jem.20021167. ISSN 0022-1007. PMC 2194102. PMID 12417631. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2194102.
^ Lukasiewicz, Randall; Velazquez-Dones Adolfo, Huynh Nhat, Hagopian Jonathan, Fu Xiang-Dong, Adams Joseph, Ghosh Gourisankar (Aug. 2007). "Structurally unique yeast and mammalian serine-arginine protein kinases catalyze evolutionarily conserved phosphorylation reactions". J. Biol. Chem. (United States) 282 (32): 23036–43. doi:10.1074/jbc.M611305200. ISSN 0021-9258. PMID 17517895.
^ Umehara, Hiroshi; Nishii Yoichi, Morishima Masaki, Kakehi Yoshiyuki, Kioka Noriyuki, Amachi Teruo, Koizumi Jun, Hagiwara Masatoshi, Ueda Kazumitsu (Feb. 2003). "Effect of cisplatin treatment on speckled distribution of a serine/arginine-rich nuclear protein CROP/Luc7A". Biochem. Biophys. Res. Commun. (United States) 301 (2): 324–9. doi:10.1016/S0006-291X(02)03017-6. ISSN 0006-291X. PMID 12565863.
^ Koizumi, J; Okamoto Y, Onogi H, Mayeda A, Krainer A R, Hagiwara M (Apr. 1999). "The subcellular localization of SF2/ASF is regulated by direct interaction with SR protein kinases (SRPKs)". J. Biol. Chem. (UNITED STATES) 274 (16): 11125–31. doi:10.1074/jbc.274.16.11125. ISSN 0021-9258. PMID 10196197.

Colwill K, Feng LL, Yeakley JM, et al. (1996). "SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors". J. Biol. Chem. 271 (40): 24569–75. doi:10.1074/jbc.271.40.24569. PMID 8798720.
Wang HY, Lin W, Dyck JA, et al. (1998). "SRPK2: A Differentially Expressed SR Protein-specific Kinase Involved in Mediating the Interaction and Localization of Pre-mRNA Splicing Factors in Mammalian Cells". J. Cell Biol. 140 (4): 737–50. doi:10.1083/jcb.140.4.737. PMC 2141757. PMID 9472028. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2141757.
Papoutsopoulou S, Nikolakaki E, Giannakouros T (1999). "SRPK1 and LBR protein kinases show identical substrate specificities". Biochem. Biophys. Res. Commun. 255 (3): 602–7. doi:10.1006/bbrc.1999.0249. PMID 10049757.
Koizumi J, Okamoto Y, Onogi H, et al. (1999). "The subcellular localization of SF2/ASF is regulated by direct interaction with SR protein kinases (SRPKs)". J. Biol. Chem. 274 (16): 11125–31. doi:10.1074/jbc.274.16.11125. PMID 10196197.
Paillard F (1999). "A novel soluble chimera for the treatment of familial hypercholesterolemia". Hum. Gene Ther. 10 (7): 1093–4. doi:10.1089/10430349950018085. PMID 10340541.
Papoutsopoulou S, Nikolakaki E, Chalepakis G, et al. (1999). "SR protein-specific kinase 1 is highly expressed in testis and phosphorylates protamine 1". Nucleic Acids Res. 27 (14): 2972–80. doi:10.1093/nar/27.14.2972. PMC 148514. PMID 10390541. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=148514.
Nikolakaki E, Kohen R, Hartmann AM, et al. (2001). "Cloning and characterization of an alternatively spliced form of SR protein kinase 1 that interacts specifically with scaffold attachment factor-B". J. Biol. Chem. 276 (43): 40175–82. doi:10.1074/jbc.M104755200. PMID 11509566.
Daub H, Blencke S, Habenberger P, et al. (2002). "Identification of SRPK1 and SRPK2 as the Major Cellular Protein Kinases Phosphorylating Hepatitis B Virus Core Protein". J. Virol. 76 (16): 8124–37. doi:10.1128/JVI.76.16.8124-8137.2002. PMC 155132. PMID 12134018. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=155132.
Kamachi M, Le TM, Kim SJ, et al. (2003). "Human Autoimmune Sera as Molecular Probes for the Identification of an Autoantigen Kinase Signaling Pathway". J. Exp. Med. 196 (9): 1213–25. doi:10.1084/jem.20021167. PMC 2194102. PMID 12417631. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2194102.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Mylonis I, Giannakouros T (2003). "Protein kinase CK2 phosphorylates and activates the SR protein-specific kinase 1". Biochem. Biophys. Res. Commun. 301 (3): 650–6. doi:10.1016/S0006-291X(02)03055-3. PMID 12565829.
Aubol BE, Chakrabarti S, Ngo J, et al. (2004). "Processive phosphorylation of alternative splicing factor/splicing factor 2". Proc. Natl. Acad. Sci. U.S.A. 100 (22): 12601–6. doi:10.1073/pnas.1635129100. PMC 240664. PMID 14555757. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=240664.
Li J, Hawkins IC, Harvey CD, et al. (2003). "Regulation of Alternative Splicing by SRrp86 and Its Interacting Proteins". Mol. Cell. Biol. 23 (21): 7437–47. doi:10.1128/MCB.23.21.7437-7447.2003. PMC 207616. PMID 14559993. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=207616.
Mungall AJ, Palmer SA, Sims SK, et al. (2003). "The DNA sequence and analysis of human chromosome 6". Nature 425 (6960): 805–11. doi:10.1038/nature02055. PMID 14574404.
Mylonis I, Drosou V, Brancorsini S, et al. (2004). "Temporal association of protamine 1 with the inner nuclear membrane protein lamin B receptor during spermiogenesis". J. Biol. Chem. 279 (12): 11626–31. doi:10.1074/jbc.M311949200. PMID 14701833.
Lee CG, Hague LK, Li H, Donnelly R (2004). "Identification of toposome, a novel multisubunit complex containing topoisomerase IIalpha". Cell Cycle 3 (5): 638–47. PMID 15034300.
Jin J, Smith FD, Stark C, et al. (2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Curr. Biol. 14 (16): 1436–50. doi:10.1016/j.cub.2004.07.051. PMID 15324660.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.

PDB gallery

1wak: X-RAY STRUCTURE OF SRPK1

1wbp: SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE

SRPK1

Interactions

References

Further reading