SPOP

This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.^[3]

References

^ ^a ^b Nagai Y, Kojima T, Muro Y, Hachiya T, Nishizawa Y, Wakabayashi T, Hagiwara M (Jan 1998). "Identification of a novel nuclear speckle-type protein, SPOP". FEBS Lett 418 (1–2): 23–6. doi:10.1016/S0014-5793(97)01340-9. PMID 9414087.
^ Zapata JM, Pawlowski K, Haas E, Ware CF, Godzik A, Reed JC (June 2001). "A diverse family of proteins containing tumor necrosis factor receptor-associated factor domains". J. Biol. Chem. 276 (26): 24242–52. doi:10.1074/jbc.M100354200. PMID 11279055.
^ ^a ^b "Entrez Gene: SPOP speckle-type POZ protein". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=8405.

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Takahashi I, Kameoka Y, Hashimoto K (2002). "MacroH2A1.2 binds the nuclear protein Spop". Biochim. Biophys. Acta 1591 (1–3): 63–8. doi:10.1016/S0167-4889(02)00249-5. PMID 12183056.
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Liu A, Desai BM, Stoffers DA (2004). "Identification of PCIF1, a POZ domain protein that inhibits PDX-1 (MODY4) transcriptional activity". Mol. Cell. Biol. 24 (10): 4372–83. doi:10.1128/MCB.24.10.4372-4383.2004. PMC 400448. PMID 15121856. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=400448.
La M, Kim K, Park J, et al. (2004). "Daxx-mediated transcriptional repression of MMP1 gene is reversed by SPOP". Biochem. Biophys. Res. Commun. 320 (3): 760–5. doi:10.1016/j.bbrc.2004.06.022. PMID 15240113.
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Hernández-Muñoz I, Lund AH, van der Stoop P, et al. (2005). "Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase". Proc. Natl. Acad. Sci. U.S.A. 102 (21): 7635–40. doi:10.1073/pnas.0408918102. PMC 1140410. PMID 15897469. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1140410.
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PDB gallery

2cr2: Solution structure of N-terminal domain of speckle-type POZ protein

SPOP

References

Further reading