SPDEF

SAM pointed domain containing ets transcription factor

PDB rendering based on 1yo5.

Available structures
PDB	1YO5, 2DKX

Identifiers

Symbols

SPDEF; PDEF; RP11-375E1__A.3; bA375E1.3

External IDs

OMIM: 608144 MGI: 1353422 HomoloGene: 8231 GeneCards: SPDEF Gene

Gene Ontology
Molecular function	• sequence-specific DNA binding transcription factor activity • sequence-specific DNA binding
Cellular component	• nucleus
Biological process	• negative regulation of transcription from RNA polymerase II promoter • multicellular organismal development • negative regulation of survival gene product expression • negative regulation of cell fate commitment • positive regulation of cell fate commitment • positive regulation of apoptosis • positive regulation of transcription from RNA polymerase II promoter • lung goblet cell differentiation • intestinal epithelial cell development
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 6:
34.51 – 34.52 Mb

Chr 17:
27.85 – 27.87 Mb

PubMed search

[1]

[2]

SAM pointed domain-containing Ets transcription factor is a protein that in humans is encoded by the SPDEF gene.^[1]^[2]^[3]

PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA (MIM 176820) expression.[supplied by OMIM]^[3]

Interactions

SPDEF has been shown to interact with NKX3-1.^[4]

References

^ Oettgen P, Finger E, Sun Z, Akbarali Y, Thamrongsak U, Boltax J, Grall F, Dube A, Weiss A, Brown L, Quinn G, Kas K, Endress G, Kunsch C, Libermann TA (Feb 2000). "PDEF, a novel prostate epithelium-specific ets transcription factor, interacts with the androgen receptor and activates prostate-specific antigen gene expression". J Biol Chem 275 (2): 1216–25. doi:10.1074/jbc.275.2.1216. PMID 10625666.
^ Johannessen JV, Finseth I (Jul 1983). "Electron micrographs in five minutes". Ultrastruct Pathol 4 (1): 97–100. doi:10.3109/01913128309140576. PMID 6857767.
^ ^a ^b "Entrez Gene: SPDEF SAM pointed domain containing ets transcription factor". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=25803.
^ Chen, Hui; Nandi Asit K, Li Xiang, Bieberich Charles J (Jan. 2002). "NKX-3.1 interacts with prostate-derived Ets factor and regulates the activity of the PSA promoter". Cancer Res. (United States) 62 (2): 338–40. ISSN 0008-5472. PMID 11809674.

Lance V (1976). "Studies on the annual reproductive cycle of the female cobra, Naja naja. Seasonal variation in plasma inorganic ions.". Comparative biochemistry and physiology. A, Comparative physiology 53 (3): 285–9. doi:10.1016/S0300-9629(76)80037-0. PMID 2437.
Yamada N, Tamai Y, Miyamoto H, Nozaki M (2000). "Cloning and expression of the mouse Pse gene encoding a novel Ets family member.". Gene 241 (2): 267–74. doi:10.1016/S0378-1119(99)00484-9. PMID 10675039.
Chen H, Nandi AK, Li X, Bieberich CJ (2002). "NKX-3.1 interacts with prostate-derived Ets factor and regulates the activity of the PSA promoter.". Cancer Res. 62 (2): 338–40. PMID 11809674.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Thompson HG, Harris JW, Wold BJ, et al. (2003). "p62 overexpression in breast tumors and regulation by prostate-derived Ets factor in breast cancer cells.". Oncogene 22 (15): 2322–33. doi:10.1038/sj.onc.1206325. PMID 12700667.
Feldman RJ, Sementchenko VI, Gayed M, et al. (2003). "Pdef expression in human breast cancer is correlated with invasive potential and altered gene expression.". Cancer Res. 63 (15): 4626–31. PMID 12907642.
Mungall AJ, Palmer SA, Sims SK, et al. (2003). "The DNA sequence and analysis of human chromosome 6.". Nature 425 (6960): 805–11. doi:10.1038/nature02055. PMID 14574404.
Ghadersohi A, Odunsi K, Lele S, et al. (2004). "Prostate derived Ets transcription factor shows better tumor-association than other cancer-associated molecules.". Oncol. Rep. 11 (2): 453–8. PMID 14719083.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Chen H, Bieberich CJ (2005). "Structural and functional analysis of domains mediating interaction between NKX-3.1 and PDEF.". J. Cell. Biochem. 94 (1): 168–77. doi:10.1002/jcb.20297. PMID 15523673.
Wang Y, Feng L, Said M, et al. (2005). "Analysis of the 2.0 A crystal structure of the protein-DNA complex of the human PDEF Ets domain bound to the prostate specific antigen regulatory site.". Biochemistry 44 (19): 7095–106. doi:10.1021/bi047352t. PMID 15882048.
Fowler M, Borazanci E, McGhee L, et al. (2006). "RUNX1 (AML-1) and RUNX2 (AML-3) cooperate with prostate-derived Ets factor to activate transcription from the PSA upstream regulatory region.". J. Cell. Biochem. 97 (1): 1–17. doi:10.1002/jcb.20664. PMID 16237704.
Gunawardane RN, Sgroi DC, Wrobel CN, et al. (2006). "Novel role for PDEF in epithelial cell migration and invasion.". Cancer Res. 65 (24): 11572–80. doi:10.1158/0008-5472.CAN-05-1196. PMC 2919290. PMID 16357167. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2919290.
Rodabaugh KJ, Mhawech-Fauceglia P, Groth J, et al. (2007). "Prostate-derived Ets factor is overexpressed in serous epithelial ovarian tumors.". Int. J. Gynecol. Pathol. 26 (1): 10–5. doi:10.1097/01.pgp.0000225386.41244.bd. PMID 17197890.
Park KS, Korfhagen TR, Bruno MD, et al. (2007). "SPDEF regulates goblet cell hyperplasia in the airway epithelium.". J. Clin. Invest. 117 (4): 978–88. doi:10.1172/JCI29176. PMC 1810569. PMID 17347682. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1810569.

PDB gallery

1yo5: Analysis of the 2.0A crystal structure of the protein-DNA complex of human PDEF Ets domain bound to the prostate specific antigen regulatory site

2dkx: Solution structure of the SAM_PNT-domain of ETS transcription factor PDEF (Prostate ets)

SPDEF

Interactions

References

Further reading