SPAG9

Sperm associated antigen 9

Identifiers

SPAG9; CT89; FLJ13450; FLJ14006; FLJ26141; FLJ34602; HLC-6; HLC4; JIP-4; JIP4; JLP; KIAA0516; MGC117291; MGC14967; MGC74461; PHET; PIG6

External IDs

OMIM: 605430 MGI: 1918084 HomoloGene: 2954 GeneCards: SPAG9 Gene

Gene Ontology
Molecular function	• protein binding • JUN kinase binding • kinesin binding • phosphatidylinositol 3-kinase binding • mitogen-activated protein kinase p38 binding
Cellular component	• acrosomal vesicle • cytoplasm • integral to membrane • cytoplasmic vesicle • perinuclear region of cytoplasm
Biological process	• activation of JUN kinase activity • spermatogenesis • positive regulation of cell migration • retrograde transport, endosome to Golgi • muscle cell differentiation • positive regulation of MAPKKK cascade • positive regulation of neuron differentiation • striated muscle cell differentiation • positive regulation of muscle cell differentiation • protein homooligomerization • negative regulation of protein homodimerization activity
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 17:
49.04 – 49.2 Mb

Chr 11:
93.86 – 93.99 Mb

PubMed search

[1]

[2]

C-jun-amino-terminal kinase-interacting protein 4 is an enzyme that in humans is encoded by the SPAG9 gene.^[1]^[2]^[3]

Extracellular signals are transduced into cells through mitogen-activated protein kinases. The structural organization of these kinases into specific signaling domains is facilitated by scaffolding proteins involved in closely tethering different kinases so that successive phosphorylation events can occur. The protein encoded by this gene is a scaffolding protein that brings together mitogen-activated protein kinases and their transcription factor targets for the activation of specific signaling pathways. This gene which is abundantly expressed in testicular haploid germ cells encodes a protein that is recognized by sperm-agglutinating antibodies and implicated in infertility.^[3]

Interactions

SPAG9 has been shown to interact with MAX.^[4]

References

^ Shankar S, Mohapatra B, Suri A (Mar 1998). "Cloning of a novel human testis mRNA specifically expressed in testicular haploid germ cells, having unique palindromic sequences and encoding a leucine zipper dimerization motif". Biochem Biophys Res Commun 243 (2): 561–5. doi:10.1006/bbrc.1997.7943. PMID 9480848.
^ Bowman AB, Kamal A, Ritchings BW, Philp AV, McGrail M, Gindhart JG, Goldstein LS (Jan 2001). "Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein". Cell 103 (4): 583–94. doi:10.1016/S0092-8674(00)00162-8. PMID 11106729.
^ ^a ^b "Entrez Gene: SPAG9 sperm associated antigen 9". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9043.
^ Lee, Clement M; Onésime Djamila, Reddy C Damodara, Dhanasekaran N, Reddy E Premkumar (Oct. 2002). "JLP: A scaffolding protein that tethers JNK/p38MAPK signaling modules and transcription factors". Proc. Natl. Acad. Sci. U.S.A. (United States) 99 (22): 14189–94. doi:10.1073/pnas.232310199. ISSN 0027-8424. PMC 137859. PMID 12391307. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=137859.

Ichijo H (1999). "From receptors to stress-activated MAP kinases.". Oncogene 18 (45): 6087–93. doi:10.1038/sj.onc.1203129. PMID 10557099.
Nakajima D, Okazaki N, Yamakawa H, et al. (2003). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones.". DNA Res. 9 (3): 99–106. doi:10.1093/dnares/9.3.99. PMID 12168954.
Nagase T, Ishikawa K, Miyajima N, et al. (1998). "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro.". DNA Res. 5 (1): 31–9. doi:10.1093/dnares/5.1.31. PMID 9628581.
Lee CM, Onésime D, Reddy CD, et al. (2002). "JLP: A scaffolding protein that tethers JNK/p38MAPK signaling modules and transcription factors.". Proc. Natl. Acad. Sci. U.S.A. 99 (22): 14189–94. doi:10.1073/pnas.232310199. PMC 137859. PMID 12391307. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=137859.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Yasuoka H, Ihn H, Medsger TA, et al. (2004). "A novel protein highly expressed in testis is overexpressed in systemic sclerosis fibroblasts and targeted by autoantibodies.". J. Immunol. 171 (12): 6883–90. PMID 14662895.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Bouwmeester T, Bauch A, Ruffner H, et al. (2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway.". Nat. Cell Biol. 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216.
Brill LM, Salomon AR, Ficarro SB, et al. (2004). "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.". Anal. Chem. 76 (10): 2763–72. doi:10.1021/ac035352d. PMID 15144186.
Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins.". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=514446.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Jagadish N, Rana R, Selvi R, et al. (2005). "Characterization of a novel human sperm-associated antigen 9 (SPAG9) having structural homology with c-Jun N-terminal kinase-interacting protein.". Biochem. J. 389 (Pt 1): 73–82. doi:10.1042/BJ20041577. PMC 1184539. PMID 15693750. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1184539.
Jagadish N, Rana R, Mishra D, et al. (2005). "Sperm associated antigen 9 (SPAG9): a new member of c-Jun NH2 -terminal kinase (JNK) interacting protein exclusively expressed in testis.". The Keio journal of medicine 54 (2): 66–71. doi:10.2302/kjm.54.66. PMID 16077255.
Guinn BA, Bland EA, Lodi U, et al. (2005). "Humoral detection of leukaemia-associated antigens in presentation acute myeloid leukaemia.". Biochem. Biophys. Res. Commun. 335 (4): 1293–304. doi:10.1016/j.bbrc.2005.08.024. PMID 16112646.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
Rana R, Jagadish N, Garg M, et al. (2006). "Small interference RNA-mediated knockdown of sperm associated antigen 9 having structural homology with c-Jun N-terminal kinase-interacting protein.". Biochem. Biophys. Res. Commun. 340 (1): 158–64. doi:10.1016/j.bbrc.2005.11.168. PMID 16356479.
Rana R, Jagadish N, Garg M, et al. (2007). "Immunogenicity study of recombinant human sperm-associated antigen 9 in bonnet macaque (Macaca radiata).". Hum. Reprod. 21 (11): 2894–900. doi:10.1093/humrep/del068. PMID 16959808.

SPAG9

Interactions

References

Further reading