SPAG6

Sperm associated antigen 6

Identifiers

SPAG6; DKFZp434I153; MGC26276; Repro-SA-1; pf16

External IDs

OMIM: 605730 HomoloGene: 8252 GeneCards: SPAG6 Gene

Gene Ontology
Molecular function	• binding
Cellular component	• cytoplasm • microtubule • cilium • axoneme • microtubule cytoskeleton • flagellum
Biological process	• spermatid development • cell projection organization
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

n/a

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 10:
22.63 – 22.74 Mb

Chr 16:
16.75 – 16.83 Mb

PubMed search

[1]

[2]

Sperm-associated antigen 6 is a protein that in humans is encoded by the SPAG6 gene.^[1]^[2]

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.^[2]

References

^ Neilson LI, Schneider PA, Van Deerlin PG, Kiriakidou M, Driscoll DA, Pellegrini MC, Millinder S, Yamamoto KK, French CK, Strauss JF 3rd (Nov 1999). "cDNA cloning and characterization of a human sperm antigen (SPAG6) with homology to the product of the Chlamydomonas PF16 locus". Genomics 60 (3): 272–80. doi:10.1006/geno.1999.5914. PMID 10493827.
^ ^a ^b "Entrez Gene: SPAG6 sperm associated antigen 6". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9576.

Diekman AB, Herr JC (1997). "Sperm antigens and their use in the development of an immunocontraceptive". Am. J. Reprod. Immunol. 37 (1): 111–7. doi:10.1111/j.1600-0897.1997.tb00199.x. PMID 9138444.
Smith EF, Lefebvre PA (1997). "The role of central apparatus components in flagellar motility and microtubule assembly". Cell Motil. Cytoskeleton 38 (1): 1–8. doi:10.1002/(SICI)1097-0169(1997)38:1<1::AID-CM1>3.0.CO;2-C. PMID 9295136.
Sapiro R, Tarantino LM, Velazquez F et al. (2000). "Sperm antigen 6 is the murine homologue of the Chlamydomonas reinhardtii central apparatus protein encoded by the PF16 locus". Biol. Reprod. 62 (3): 511–8. doi:10.1095/biolreprod62.3.511. PMID 10684790.
Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=310948.
Wiemann S, Weil B, Wellenreuther R et al. (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=311072.
Sapiro R, Kostetskii I, Olds-Clarke P et al. (2002). "Male Infertility, Impaired Sperm Motility, and Hydrocephalus in Mice Deficient in Sperm-Associated Antigen 6". Mol. Cell. Biol. 22 (17): 6298–305. doi:10.1128/MCB.22.17.6298-6305.2002. PMC 134010. PMID 12167721. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=134010.
Zhang Z, Sapiro R, Kapfhamer D et al. (2002). "A Sperm-Associated WD Repeat Protein Orthologous to Chlamydomonas PF20 Associates with Spag6, the Mammalian Orthologue of Chlamydomonas PF16". Mol. Cell. Biol. 22 (22): 7993–8004. doi:10.1128/MCB.22.22.7993-8004.2002. PMC 134734. PMID 12391165. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=134734.
Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Ficarro S, Chertihin O, Westbrook VA et al. (2003). "Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation". J. Biol. Chem. 278 (13): 11579–89. doi:10.1074/jbc.M202325200. PMID 12509440.
Deloukas P, Earthrowl ME, Grafham DV et al. (2004). "The DNA sequence and comparative analysis of human chromosome 10". Nature 429 (6990): 375–81. doi:10.1038/nature02462. PMID 15164054.
Gerhard DS, Wagner L, Feingold EA et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Wiemann S, Arlt D, Huber W et al. (2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528930.
Mehrle A, Rosenfelder H, Schupp I et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1347501.

SPAG6

References

Further reading