SF3B2

Splicing factor 3b, subunit 2, 145kDa

PDB rendering based on 2do5.

Available structures
PDB	2do5

Identifiers

Symbols

SF3B2; Cus1; SAP145; SF3B145; SF3b1; SF3b150

External IDs

OMIM: 605591 MGI: 2441856 HomoloGene: 6678 GeneCards: SF3B2 Gene

Gene Ontology
Molecular function	• nucleic acid binding • protein binding
Cellular component	• nucleus • nucleoplasm • spliceosomal complex • U12-type spliceosomal complex • catalytic step 2 spliceosome
Biological process	• nuclear mRNA splicing, via spliceosome • nuclear mRNA splicing, via spliceosome • mRNA processing • RNA splicing • gene expression • interspecies interaction between organisms
Sources: Amigo / QuickGO

Orthologs

Species

Human

Mouse

n/a

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 11:
65.82 – 65.84 Mb

Chr 19:
5.27 – 5.3 Mb

PubMed search

[1]

[2]

Splicing factor 3B subunit 2 is a protein that in humans is encoded by the SF3B2 gene.^[1]^[2]

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.^[2]

Interactions

SF3B2 has been shown to interact with SF3B4,^[3]^[4] RBM7,^[5] SF3B1^[6]^[7] and CDC5L.^[8]

References

^ Gozani O, Feld R, Reed R (Mar 1996). "Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A". Genes Dev 10 (2): 233–43. doi:10.1101/gad.10.2.233. PMID 8566756.
^ ^a ^b "Entrez Gene: SF3B2 splicing factor 3b, subunit 2, 145kDa". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=10992.
^ Rual, Jean-François; Venkatesan Kavitha, Hao Tong, Hirozane-Kishikawa Tomoko, Dricot Amélie, Li Ning, Berriz Gabriel F, Gibbons Francis D, Dreze Matija, Ayivi-Guedehoussou Nono, Klitgord Niels, Simon Christophe, Boxem Mike, Milstein Stuart, Rosenberg Jennifer, Goldberg Debra S, Zhang Lan V, Wong Sharyl L, Franklin Giovanni, Li Siming, Albala Joanna S, Lim Janghoo, Fraughton Carlene, Llamosas Estelle, Cevik Sebiha, Bex Camille, Lamesch Philippe, Sikorski Robert S, Vandenhaute Jean, Zoghbi Huda Y, Smolyar Alex, Bosak Stephanie, Sequerra Reynaldo, Doucette-Stamm Lynn, Cusick Michael E, Hill David E, Roth Frederick P, Vidal Marc (Oct. 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature (England) 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
^ Champion-Arnaud, P; Reed R (Aug. 1994). "The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site". Genes Dev. (UNITED STATES) 8 (16): 1974–83. doi:10.1101/gad.8.16.1974. ISSN 0890-9369. PMID 7958871.
^ Guo, Taylor B; Boros Laszlo G, Chan Kam C, Hikim Amiya P Sinha, Hudson Alan P, Swerdloff Ronald S, Mitchell Aaron P, Salameh Wael A (2003). "Spermatogenetic expression of RNA-binding motif protein 7, a protein that interacts with splicing factors". J. Androl. (United States) 24 (2): 204–14. ISSN 0196-3635. PMID 12634307.
^ Das, B K; Xia L, Palandjian L, Gozani O, Chyung Y, Reed R (Oct. 1999). "Characterization of a Protein Complex Containing Spliceosomal Proteins SAPs 49, 130, 145, and 155". Mol. Cell. Biol. (UNITED STATES) 19 (10): 6796–802. ISSN 0270-7306. PMC 84676. PMID 10490618. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=84676.
^ Will, Cindy L; Urlaub Henning, Achsel Tilmann, Gentzel Marc, Wilm Matthias, Lührmann Reinhard (Sep. 2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein". EMBO J. (England) 21 (18): 4978–88. doi:10.1093/emboj/cdf480. ISSN 0261-4189. PMC 126279. PMID 12234937. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=126279.
^ Ajuh, P; Kuster B, Panov K, Zomerdijk J C, Mann M, Lamond A I (Dec. 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". EMBO J. (ENGLAND) 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. ISSN 0261-4189. PMC 305846. PMID 11101529. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=305846.

Agell N, Aligué R, Alemany V et al. (1998). "New nuclear functions for calmodulin". Cell Calcium 23 (2–3): 115–21. doi:10.1016/S0143-4160(98)90109-9. PMID 9601606.
Champion-Arnaud P, Reed R (1994). "The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site". Genes Dev. 8 (16): 1974–83. doi:10.1101/gad.8.16.1974. PMID 7958871.
Neubauer G, King A, Rappsilber J et al. (1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex". Nat. Genet. 20 (1): 46–50. doi:10.1038/1700. PMID 9731529.
Das BK, Xia L, Palandjian L et al. (2000). "Characterization of a Protein Complex Containing Spliceosomal Proteins SAPs 49, 130, 145, and 155". Mol. Cell. Biol. 19 (10): 6796–802. PMC 84676. PMID 10490618. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=84676.
Das R, Zhou Z, Reed R (2000). "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E". Mol. Cell 5 (5): 779–87. doi:10.1016/S1097-2765(00)80318-4. PMID 10882114.
Bryant HE, Wadd SE, Lamond AI et al. (2001). "Herpes Simplex Virus IE63 (ICP27) Protein Interacts with Spliceosome-Associated Protein 145 and Inhibits Splicing prior to the First Catalytic Step". J. Virol. 75 (9): 4376–85. doi:10.1128/JVI.75.9.4376-4385.2001. PMC 114182. PMID 11287586. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=114182.
Brand M, Moggs JG, Oulad-Abdelghani M et al. (2001). "UV-damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation". EMBO J. 20 (12): 3187–96. doi:10.1093/emboj/20.12.3187. PMC 150203. PMID 11406595. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=150203.
Will CL, Schneider C, MacMillan AM et al. (2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site". EMBO J. 20 (16): 4536–46. doi:10.1093/emboj/20.16.4536. PMC 125580. PMID 11500380. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=125580.
Jurica MS, Licklider LJ, Gygi SR et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1370266.
Will CL, Urlaub H, Achsel T et al. (2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein". EMBO J. 21 (18): 4978–88. doi:10.1093/emboj/cdf480. PMC 126279. PMID 12234937. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=126279.
Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
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Will CL, Schneider C, Hossbach M et al. (2004). "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome". RNA 10 (6): 929–41. doi:10.1261/rna.7320604. PMC 1370585. PMID 15146077. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1370585.
Beausoleil SA, Jedrychowski M, Schwartz D et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=514446.
Lin KT, Lu RM, Tarn WY (2004). "The WW Domain-Containing Proteins Interact with the Early Spliceosome and Participate in Pre-mRNA Splicing In Vivo". Mol. Cell. Biol. 24 (20): 9176–85. doi:10.1128/MCB.24.20.9176-9185.2004. PMC 517884. PMID 15456888. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=517884.
Andersen JS, Lam YW, Leung AK et al. (2005). "Nucleolar proteome dynamics". Nature 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413.
Rual JF, Venkatesan K, Hao T et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
Ardon O, Zimmerman ES, Andersen JL et al. (2006). "Induction of G2 Arrest and Binding to Cyclophilin A Are Independent Phenotypes of Human Immunodeficiency Virus Type 1 Vpr". J. Virol. 80 (8): 3694–700. doi:10.1128/JVI.80.8.3694-3700.2006. PMC 1440437. PMID 16571786. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1440437.

PDB gallery

2do5: Solution structure of the SAP domain of human splicing factor 3B subunit 2

SF3B2

Interactions

References

Further reading