SF3A3

Splicing factor 3a, subunit 3, 60kDa

PDB rendering based on 2dt7.

Available structures
PDB	2dt7

Identifiers

Symbols

SF3A3; PRP9; PRPF9; SAP61; SF3a60

External IDs

OMIM: 605596 MGI: 1922312 HomoloGene: 4949 GeneCards: SF3A3 Gene

Gene Ontology
Molecular function	• nucleic acid binding • protein binding • zinc ion binding • metal ion binding
Cellular component	• intracellular • nucleus • nucleoplasm • spliceosomal complex • nucleolus • nuclear speck • catalytic step 2 spliceosome
Biological process	• RNA splicing, via transesterification reactions • nuclear mRNA 3'-splice site recognition • nuclear mRNA splicing, via spliceosome • nuclear mRNA splicing, via spliceosome • mRNA processing • RNA splicing • gene expression
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

n/a

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 1:
38.42 – 38.46 Mb

Chr 4:
124.39 – 124.41 Mb

PubMed search

[1]

[2]

Splicing factor 3A subunit 3 is a protein that in humans is encoded by the SF3A3 gene.^[1]^[2]^[3]

This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20.^[3]

Interactions

SF3A3 has been shown to interact with SF3A1.^[2]^[4]

References

^ Kramer A, Legrain P, Mulhauser F, Groning K, Brosi R, Bilbe G (Feb 1995). "Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo". Nucleic Acids Res 22 (24): 5223–8. doi:10.1093/nar/22.24.5223. PMC 332064. PMID 7816610. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=332064.
^ ^a ^b Chiara MD, Champion-Arnaud P, Buvoli M, Nadal-Ginard B, Reed R (Aug 1994). "Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114". Proc Natl Acad Sci U S A 91 (14): 6403–7. doi:10.1073/pnas.91.14.6403. PMC 44210. PMID 8022796. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=44210.
^ ^a ^b "Entrez Gene: SF3A3 splicing factor 3a, subunit 3, 60kDa". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=10946.
^ Nesic, D; Krämer A (Oct. 2001). "Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation". Mol. Cell. Biol. (United States) 21 (19): 6406–17. doi:10.1128/MCB.21.19.6406-6417.2001. ISSN 0270-7306. PMC 99788. PMID 11533230. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=99788.

Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Neubauer G, King A, Rappsilber J, et al. (1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex". Nat. Genet. 20 (1): 46–50. doi:10.1038/1700. PMID 9731529.
Das R, Zhou Z, Reed R (2000). "Functional association of U2 snRNP with the ATP-independent spliceosomal complex E". Mol. Cell 5 (5): 779–87. doi:10.1016/S1097-2765(00)80318-4. PMID 10882114.
Will CL, Schneider C, MacMillan AM, et al. (2001). "A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site". EMBO J. 20 (16): 4536–46. doi:10.1093/emboj/20.16.4536. PMC 125580. PMID 11500380. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=125580.
Nesic D, Krämer A (2001). "Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation". Mol. Cell. Biol. 21 (19): 6406–17. doi:10.1128/MCB.21.19.6406-6417.2001. PMC 99788. PMID 11533230. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=99788.
Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1370266.
Will CL, Urlaub H, Achsel T, et al. (2002). "Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein". EMBO J. 21 (18): 4978–88. doi:10.1093/emboj/cdf480. PMC 126279. PMID 12234937. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=126279.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Dubois T, Zemlickova E, Howell S, Aitken A (2003). "Centaurin-alpha 1 associates in vitro and in vivo with nucleolin". Biochem. Biophys. Res. Commun. 301 (2): 502–8. doi:10.1016/S0006-291X(02)03010-3. PMID 12565890.
Li J, Hawkins IC, Harvey CD, et al. (2003). "Regulation of alternative splicing by SRrp86 and its interacting proteins". Mol. Cell. Biol. 23 (21): 7437–47. doi:10.1128/MCB.23.21.7437-7447.2003. PMC 207616. PMID 14559993. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=207616.
Nesic D, Tanackovic G, Krämer A (2005). "A role for Cajal bodies in the final steps of U2 snRNP biogenesis". J. Cell. Sci. 117 (Pt 19): 4423–33. doi:10.1242/jcs.01308. PMID 15316075.
Lin KT, Lu RM, Tarn WY (2004). "The WW domain-containing proteins interact with the early spliceosome and participate in pre-mRNA splicing in vivo". Mol. Cell. Biol. 24 (20): 9176–85. doi:10.1128/MCB.24.20.9176-9185.2004. PMC 517884. PMID 15456888. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=517884.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455.
Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413.
Tanackovic G, Krämer A (2005). "Human splicing factor SF3a, but not SF1, is essential for pre-mRNA splicing in vivo". Mol. Biol. Cell 16 (3): 1366–77. doi:10.1091/mbc.E04-11-1034. PMC 551499. PMID 15647371. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=551499.

PDB gallery

2dt7: Solution structure of the second SURP domain of human splicing factor SF3a120 in complex with a fragment of human splicing factor SF3a60

SF3A3

Interactions

References

Further reading