SF3A1

Splicing factor 3a, subunit 1, 120kDa

PDB rendering based on 1we7.
Identifiers
Symbols SF3A1; PRP21; PRPF21; SAP114; SF3A120
External IDs OMIM: 605595 MGI: 1914715 HomoloGene: 4294 GeneCards: SF3A1 Gene
RNA expression pattern
More reference expression data
Orthologs
Species Human Mouse
Entrez 10291 67465
Ensembl ENSG00000099995 ENSMUSG00000002129
UniProt Q15459 Q3TVM1
RefSeq (mRNA) NM_001005409.1 NM_026175.5
RefSeq (protein) NP_001005409.1 NP_080451.4
Location (UCSC) Chr 22:
30.73 – 30.75 Mb		 Chr 11:
4.06 – 4.08 Mb
PubMed search [1] [2]

Splicing factor 3 subunit 1 is a protein that in humans is encoded by the SF3A1 gene.[1][2]

This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.[2]

Interactions

SF3A1 has been shown to interact with SF3A3[3][4] and CDC5L.[5]

References

  1. ^ Kramer A, Mulhauser F, Wersig C, Groning K, Bilbe G (Jan 1996). "Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of Saccharomyces cerevisiae". RNA 1 (3): 260–72. PMC 1369079. PMID 7489498. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1369079. 
  2. ^ a b "Entrez Gene: SF3A1 splicing factor 3a, subunit 1, 120kDa". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=10291. 
  3. ^ Nesic, D; Krämer A (Oct. 2001). "Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation". Mol. Cell. Biol. (United States) 21 (19): 6406–17. doi:10.1128/MCB.21.19.6406-6417.2001. ISSN 0270-7306. PMC 99788. PMID 11533230. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=99788. 
  4. ^ Chiara, M D; Champion-Arnaud P, Buvoli M, Nadal-Ginard B, Reed R (Jul. 1994). "Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 91 (14): 6403–7. doi:10.1073/pnas.91.14.6403. ISSN 0027-8424. PMC 44210. PMID 8022796. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=44210. 
  5. ^ Ajuh, P; Kuster B, Panov K, Zomerdijk J C, Mann M, Lamond A I (Dec. 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". EMBO J. (ENGLAND) 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. ISSN 0261-4189. PMC 305846. PMID 11101529. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=305846. 

Further reading