SETDB1

SET domain, bifurcated 1

Identifiers

SETDB1; ESET; H3-K9-HMTase4; KG1T; KIAA0067; KMT1E

External IDs

OMIM: 604396 MGI: 1934229 HomoloGene: 32157 GeneCards: SETDB1 Gene

2.1.1.43

Gene Ontology
Molecular function	• DNA binding • protein binding • methyltransferase activity • zinc ion binding • transferase activity • histone-lysine N-methyltransferase activity
Cellular component	• nucleus • chromosome • nucleolus • cytoplasm • Golgi apparatus • plasma membrane
Biological process	• regulation of transcription, DNA-dependent • chromatin modification
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 1:
150.9 – 150.94 Mb

Chr 3:
95.13 – 95.16 Mb

PubMed search

[1]

[2]

Histone-lysine N-methyltransferase SETDB1 is an enzyme that in humans is encoded by the SETDB1 gene.^[1]^[2]

1 Function
2 Interactions
3 References
4 Further reading
5 External links

Function

The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities.^[2]

Interactions

SETDB1 has been shown to interact with TRIM28.^[3]

References

^ Harte PJ, Wu W, Carrasquillo MM, Matera AG (Jun 1999). "Assignment of a novel bifurcated SET domain gene, SETDB1, to human chromosome band 1q21 by in situ hybridization and radiation hybrids". Cytogenet Cell Genet 84 (1–2): 83–6. doi:10.1159/000015220. PMID 10343109.
^ ^a ^b "Entrez Gene: SETDB1 SET domain, bifurcated 1". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9869.
^ Schultz, David C; Ayyanathan Kasirajan, Negorev Dmitri, Maul Gerd G, Rauscher Frank J (Apr. 2002). "SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins". Genes Dev. (United States) 16 (8): 919–32. doi:10.1101/gad.973302. ISSN 0890-9369. PMC 152359. PMID 11959841. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=152359.

Nomura N, Nagase T, Miyajima N, et al. (1995). "Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1". DNA Res. 1 (5): 223–9. doi:10.1093/dnares/1.5.223. PMID 7584044.
Yang L, Xia L, Wu DY, et al. (2002). "Molecular cloning of ESET, a novel histone H3-specific methyltransferase that interacts with ERG transcription factor". Oncogene 21 (1): 148–52. doi:10.1038/sj.onc.1204998. PMID 11791185.
Schultz DC, Ayyanathan K, Negorev D, et al. (2002). "SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins". Genes Dev. 16 (8): 919–32. doi:10.1101/gad.973302. PMC 152359. PMID 11959841. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=152359.
Yang L, Mei Q, Zielinska-Kwiatkowska A, et al. (2003). "An ERG (ets-related gene)-associated histone methyltransferase interacts with histone deacetylases 1/2 and transcription co-repressors mSin3A/B". Biochem. J. 369 (Pt 3): 651–7. doi:10.1042/BJ20020854. PMC 1223118. PMID 12398767. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1223118.
Nakayama M, Kikuno R, Ohara O (2003). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=187542.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Ayyanathan K, Lechner MS, Bell P, et al. (2003). "Regulated recruitment of HP1 to a euchromatic gene induces mitotically heritable, epigenetic gene silencing: a mammalian cell culture model of gene variegation". Genes Dev. 17 (15): 1855–69. doi:10.1101/gad.1102803. PMC 196232. PMID 12869583. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=196232.
Wang H, An W, Cao R, et al. (2003). "mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression". Mol. Cell 12 (2): 475–87. doi:10.1016/j.molcel.2003.08.007. PMID 14536086.
Paces-Fessy M, Boucher D, Petit E, et al. (2004). "The negative regulator of Gli, Suppressor of fused (Sufu), interacts with SAP18, Galectin3 and other nuclear proteins". Biochem. J. 378 (Pt 2): 353–62. doi:10.1042/BJ20030786. PMC 1223961. PMID 14611647. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1223961.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Colland F, Jacq X, Trouplin V, et al. (2004). "Functional proteomics mapping of a human signaling pathway". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=442148.
Sarraf SA, Stancheva I (2004). "Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly". Mol. Cell 15 (4): 595–605. doi:10.1016/j.molcel.2004.06.043. PMID 15327775.
Goehler H, Lalowski M, Stelzl U, et al. (2004). "A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease". Mol. Cell 15 (6): 853–65. doi:10.1016/j.molcel.2004.09.016. PMID 15383276.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Ichimura T, Watanabe S, Sakamoto Y, et al. (2005). "Transcriptional repression and heterochromatin formation by MBD1 and MCAF/AM family proteins". J. Biol. Chem. 280 (14): 13928–35. doi:10.1074/jbc.M413654200. PMID 15691849.
Verschure PJ, van der Kraan I, de Leeuw W, et al. (2005). "In vivo HP1 targeting causes large-scale chromatin condensation and enhanced histone lysine methylation". Mol. Cell. Biol. 25 (11): 4552–64. doi:10.1128/MCB.25.11.4552-4564.2005. PMC 1140641. PMID 15899859. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1140641.
Gevaert K, Staes A, Van Damme J, et al. (2006). "Global phosphoproteome analysis on human HepG2 hepatocytes using reversed-phase diagonal LC". Proteomics 5 (14): 3589–99. doi:10.1002/pmic.200401217. PMID 16097034.
Stelzl U, Worm U, Lalowski M, et al. (2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. PMID 16169070.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.

External links

FactorBook SETDB1

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

SETDB1

Contents

Function

Interactions

References

Further reading

External links