SAE1

SUMO1 activating enzyme subunit 1

PDB rendering based on 1y8q.

Available structures
PDB	1Y8Q, 1Y8R, 3KYC, 3KYD

Identifiers

Symbols

SAE1; AOS1; FLJ3091; HSPC140; SUA1; UBLE1A

External IDs

OMIM: 613294 MGI: 1929264 HomoloGene: 4019 GeneCards: SAE1 Gene

Gene Ontology
Molecular function	• ubiquitin activating enzyme activity • protein binding • protein C-terminus binding • enzyme activator activity • ligase activity • SUMO activating enzyme activity • ATP-dependent protein binding • protein heterodimerization activity • protein heterodimerization activity
Cellular component	• nucleus
Biological process	• protein modification process • protein ubiquitination • protein sumoylation • positive regulation of catalytic activity
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 19:
47.63 – 47.71 Mb

Chr 7:
16.91 – 16.97 Mb

PubMed search

[1]

[2]

SUMO-activating enzyme subunit 1 is a protein that in humans is encoded by the SAE1 gene.^[1]^[2]^[3]^[4]

Interactions

SAE1 has been shown to interact with SAE2.^[5]^[6]^[3]^[1]

References

^ ^a ^b Desterro JM, Rodriguez MS, Kemp GD, Hay RT (May 1999). "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1". J Biol Chem 274 (15): 10618–24. doi:10.1074/jbc.274.15.10618. PMID 10187858.
^ Okuma T, Honda R, Ichikawa G, Tsumagari N, Yasuda H (Mar 1999). "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2". Biochem Biophys Res Commun 254 (3): 693–8. doi:10.1006/bbrc.1998.9995. PMID 9920803.
^ ^a ^b Gong L, Li B, Millas S, Yeh ET (Jun 1999). "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex". FEBS Lett 448 (1): 185–9. doi:10.1016/S0014-5793(99)00367-1. PMID 10217437.
^ "Entrez Gene: SAE1 SUMO1 activating enzyme subunit 1". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=10055.
^ Rual, Jean-François; Venkatesan Kavitha, Hao Tong, Hirozane-Kishikawa Tomoko, Dricot Amélie, Li Ning, Berriz Gabriel F, Gibbons Francis D, Dreze Matija, Ayivi-Guedehoussou Nono, Klitgord Niels, Simon Christophe, Boxem Mike, Milstein Stuart, Rosenberg Jennifer, Goldberg Debra S, Zhang Lan V, Wong Sharyl L, Franklin Giovanni, Li Siming, Albala Joanna S, Lim Janghoo, Fraughton Carlene, Llamosas Estelle, Cevik Sebiha, Bex Camille, Lamesch Philippe, Sikorski Robert S, Vandenhaute Jean, Zoghbi Huda Y, Smolyar Alex, Bosak Stephanie, Sequerra Reynaldo, Doucette-Stamm Lynn, Cusick Michael E, Hill David E, Roth Frederick P, Vidal Marc (Oct. 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature (England) 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
^ Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1847948.

Zhang QH, Ye M, Wu XY, et al. (2001). "Cloning and Functional Analysis of cDNAs with Open Reading Frames for 300 Previously Undefined Genes Expressed in CD34+ Hematopoietic Stem/Progenitor Cells". Genome Res. 10 (10): 1546–60. doi:10.1101/gr.140200. PMC 310934. PMID 11042152. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=310934.
Tatham MH, Jaffray E, Vaughan OA, et al. (2001). "Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9". J. Biol. Chem. 276 (38): 35368–74. doi:10.1074/jbc.M104214200. PMID 11451954.
Azuma Y, Tan SH, Cavenagh MM, et al. (2001). "Expression and regulation of the mammalian SUMO-1 E1 enzyme". FASEB J. 15 (10): 1825–7. PMID 11481243.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Tatham MH, Kim S, Yu B, et al. (2003). "Role of an N-terminal site of Ubc9 in SUMO-1, -2, and -3 binding and conjugation". Biochemistry 42 (33): 9959–69. doi:10.1021/bi0345283. PMID 12924945.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Komatsu T, Mizusaki H, Mukai T, et al. (2005). "Small ubiquitin-like modifier 1 (SUMO-1) modification of the synergy control motif of Ad4 binding protein/steroidogenic factor 1 (Ad4BP/SF-1) regulates synergistic transcription between Ad4BP/SF-1 and Sox9". Mol. Endocrinol. 18 (10): 2451–62. doi:10.1210/me.2004-0173. PMID 15192080.
Salinas S, Briançon-Marjollet A, Bossis G, et al. (2004). "SUMOylation regulates nucleo-cytoplasmic shuttling of Elk-1". J. Cell Biol. 165 (6): 767–73. doi:10.1083/jcb.200310136. PMC 2172397. PMID 15210726. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2172397.
Lin JY, Ohshima T, Shimotohno K (2004). "Association of Ubc9, an E2 ligase for SUMO conjugation, with p53 is regulated by phosphorylation of p53". FEBS Lett. 573 (1–3): 15–8. doi:10.1016/j.febslet.2004.07.059. PMID 15327968.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Boggio R, Colombo R, Hay RT, et al. (2005). "A mechanism for inhibiting the SUMO pathway". Mol. Cell 16 (4): 549–61. doi:10.1016/j.molcel.2004.11.007. PMID 15546615.
Lois LM, Lima CD (2005). "Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1". EMBO J. 24 (3): 439–51. doi:10.1038/sj.emboj.7600552. PMC 548657. PMID 15660128. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=548657.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
Klenk C, Humrich J, Quitterer U, Lohse MJ (2006). "SUMO-1 controls the protein stability and the biological function of phosducin". J. Biol. Chem. 281 (13): 8357–64. doi:10.1074/jbc.M513703200. PMID 16421094.
Li T, Santockyte R, Shen RF, et al. (2006). "A general approach for investigating enzymatic pathways and substrates for ubiquitin-like modifiers". Arch. Biochem. Biophys. 453 (1): 70–4. doi:10.1016/j.abb.2006.03.002. PMID 16620772.
Pan X, Li H, Zhang P, et al. (2006). "Ubc9 interacts with SOX4 and represses its transcriptional activity". Biochem. Biophys. Res. Commun. 344 (3): 727–34. doi:10.1016/j.bbrc.2006.03.194. PMID 16631117.
Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1847948.

PDB gallery

1y8q: SUMO E1 ACTIVATING ENZYME SAE1-SAE2-MG-ATP COMPLEX

1y8r: SUMO E1 ACTIVATING ENZYME SAE1-SAE2-SUMO1-MG-ATP COMPLEX

SAE1

Interactions

References

Further reading