RsaOG

rsaOG RNA
The consensus secondary structure of RsaOG showing its pseudoknot, created in Varna.[1] Boundaries were determined by RACE mapping in Staphylococcus aureus N315.[2]
Identifiers
Symbol RsaOG
Rfam RF01775
Other data
RNA type sRNA
Domain(s) Staphylococcus

RsaOG (an acronym for RNA S. aureus Orsay G)[2] is a non-coding RNA that was discovered in the pathogenic bacteria Staphylococcus aureus N315 using a large scale computational screening based on phylogenetic profiling.[3] It was first identified, but not named, in 2005.[4] RsaOG has since been identified in other strains of Staphylococcus aureus under the name of RsaI,[5] it has also been discovered in other members of the Staphylococcus genus (such as Staphylococcus carnosus) but in no other bacteria.[2]

The RsaOG gene is conserved in all Staphylococcaceae sequenced genomes, its secondary structure contains two highly conserved unpaired sequences which have the ability to form a pseudoknot.[2] Northern blot experiments show that RsaOG is expressed in several S. aureus strains.[3][5] Mapping of RsaOG ends indicates a size of 146 nucleotides in S. aureus.[5] RsaOG ncRNA is thought to have trans-acting regulatory functions, possibly on fine tuning toxin production or aiding with invasion.[2]

See Also

References

  1. ^ Darty K, Denise A, Ponty Y (August 2009). "VARNA: Interactive drawing and editing of the RNA secondary structure". Bioinformatics 25 (15): 1974–5. doi:10.1093/bioinformatics/btp250. PMC 2712331. PMID 19398448. http://bioinformatics.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=19398448. Retrieved 2010-07-12. 
  2. ^ a b c d e Marchais A, Bohn C, Bouloc P, Gautheret D (March 2010). "RsaOG, a new staphylococcal family of highly transcribed non-coding RNA". RNA Biol 7 (2): 116–9. PMID 20200491. http://www.landesbioscience.com/journals/rna/abstract.php?id=10925. Retrieved 2010-07-12. 
  3. ^ a b Marchais A, Naville M, Bohn C, Bouloc P, Gautheret D (June 2009). "Single-pass classification of all noncoding sequences in a bacterial genome using phylogenetic profiles". Genome Res. 19 (6): 1084–92. doi:10.1101/gr.089714.108. PMC 2694484. PMID 19237465. http://genome.cshlp.org/cgi/pmidlookup?view=long&pmid=19237465. Retrieved 2010-07-12. 
  4. ^ Pichon C, Felden B (October 2005). "Small RNA genes expressed from Staphylococcus aureus genomic and pathogenicity islands with specific expression among pathogenic strains". Proc. Natl. Acad. Sci. U.S.A. 102 (40): 14249–54. doi:10.1073/pnas.0503838102. PMC 1242290. PMID 16183745. http://www.pnas.org/cgi/pmidlookup?view=long&pmid=16183745. Retrieved 2010-07-12. 
  5. ^ a b c Geissmann T, Chevalier C, Cros MJ, et al. (November 2009). "A search for small noncoding RNAs in Staphylococcus aureus reveals a conserved sequence motif for regulation". Nucleic Acids Res. 37 (21): 7239–57. doi:10.1093/nar/gkp668. PMC 2790875. PMID 19786493. http://nar.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=19786493. Retrieved 2010-07-12. 

External links