RPA3

Replication protein A3, 14kDa

PDB rendering based on 1l1o.

Available structures
PDB	1L1O, 1QUQ, 2PI2, 2PQA, 2Z6K, 3KDF

Identifiers

Symbols

RPA3; REPA3

External IDs

OMIM: 179837 MGI: 1915490 HomoloGene: 68285 GeneCards: RPA3 Gene

Gene Ontology
Molecular function	• single-stranded DNA binding • protein binding
Cellular component	• nucleus • nucleoplasm • nucleoplasm • DNA replication factor A complex • cytoplasm
Biological process	• cell cycle checkpoint • G1/S transition of mitotic cell cycle • S phase of mitotic cell cycle • M/G1 transition of mitotic cell cycle • mitotic cell cycle • nucleotide-excision repair, DNA damage removal • telomere maintenance via recombination • telomere maintenance • double-strand break repair via homologous recombination • DNA recombinase assembly • DNA replication • DNA strand elongation involved in DNA replication • DNA repair • transcription-coupled nucleotide-excision repair • nucleotide-excision repair • nucleotide-excision repair, DNA gap filling • double-strand break repair • DNA recombination • telomere maintenance via semi-conservative replication
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 7:
7.68 – 7.76 Mb

Chr 6:
8.21 – 8.21 Mb

PubMed search

[1]

[2]

Replication protein A 14 kDa subunit is a protein that in humans is encoded by the RPA3 gene.^[1]^[2]

Interactions

RPA3 has been shown to interact with Replication protein A1^[3]^[4] and RPA2.^[3]^[4]

References

^ Umbricht CB, Erdile LF, Jabs EW, Kelly TJ (Apr 1993). "Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A". J Biol Chem 268 (9): 6131–8. PMID 8454588.
^ "Entrez Gene: RPA3 replication protein A3, 14kDa". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=6119.
^ ^a ^b Bochkareva, E; Frappier L, Edwards A M, Bochkarev A (Feb. 1998). "The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain". J. Biol. Chem. (UNITED STATES) 273 (7): 3932–6. doi:10.1074/jbc.273.7.3932. ISSN 0021-9258. PMID 9461578.
^ ^a ^b Bochkareva, Elena; Korolev Sergey, Lees-Miller Susan P, Bochkarev Alexey (Apr. 2002). "Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA". EMBO J. (England) 21 (7): 1855–63. doi:10.1093/emboj/21.7.1855. ISSN 0261-4189. PMC 125950. PMID 11927569. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=125950.

Iftode C, Daniely Y, Borowiec JA (1999). "Replication protein A (RPA): the eukaryotic SSB.". Crit. Rev. Biochem. Mol. Biol. 34 (3): 141–80. doi:10.1080/10409239991209255. PMID 10473346.
Keshav KF, Chen C, Dutta A (1995). "Rpa4, a homolog of the 34-kilodalton subunit of the replication protein A complex.". Mol. Cell. Biol. 15 (6): 3119–28. PMC 230543. PMID 7760808. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=230543.
Umbricht CB, Griffin CA, Hawkins AL, et al. (1994). "High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3).". Genomics 20 (2): 249–57. doi:10.1006/geno.1994.1161. PMID 8020972.
Amacker M, Hottiger M, Mossi R, Hübscher U (1997). "HIV-1 nucleocapsid protein and replication protein A influence the strand displacement DNA synthesis of lentiviral reverse transcriptase.". AIDS 11 (4): 534–6. PMID 9084803.
Bochkareva E, Frappier L, Edwards AM, Bochkarev A (1998). "The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain.". J. Biol. Chem. 273 (7): 3932–6. doi:10.1074/jbc.273.7.3932. PMID 9461578.
"Toward a complete human genome sequence.". Genome Res. 8 (11): 1097–108. 1999. doi:10.1101/gr.8.11.1097. PMID 9847074.
Mer G, Bochkarev A, Gupta R, et al. (2000). "Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA.". Cell 103 (3): 449–56. doi:10.1016/S0092-8674(00)00136-7. PMID 11081631.
Habel JE, Ohren JF, Borgstahl GE (2001). "Dynamic light-scattering analysis of full-length human RPA14/32 dimer: purification, crystallization and self-association.". Acta Crystallogr. D Biol. Crystallogr. 57 (Pt 2): 254–9. doi:10.1107/S0907444900015225. PMID 11173472.
Bochkareva E, Korolev S, Lees-Miller SP, Bochkarev A (2002). "Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA.". EMBO J. 21 (7): 1855–63. doi:10.1093/emboj/21.7.1855. PMC 125950. PMID 11927569. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=125950.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Kneissl M, Pütter V, Szalay AA, Grummt F (2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells.". J. Mol. Biol. 327 (1): 111–28. doi:10.1016/S0022-2836(03)00079-2. PMID 12614612.
Scherer SW, Cheung J, MacDonald JR, et al. (2003). "Human chromosome 7: DNA sequence and biology.". Science 300 (5620): 767–72. doi:10.1126/science.1083423. PMC 2882961. PMID 12690205. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2882961.
Hillier LW, Fulton RS, Fulton LA, et al. (2003). "The DNA sequence of human chromosome 7.". Nature 424 (6945): 157–64. doi:10.1038/nature01782. PMID 12853948.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry.". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1847948.

PDB gallery

1l1o: Structure of the human Replication Protein A (RPA) trimerization core

1quq: COMPLEX OF REPLICATION PROTEIN A SUBUNITS RPA14 AND RPA32

RPA3

Interactions

References

Further reading