RAPGEF2

Rap guanine nucleotide exchange factor (GEF) 2

Identifiers

RAPGEF2; CNrasGEF; NRAPGEP; PDZ-GEF1; PDZGEF1; RA-GEF; Rap-GEP; nRap GEP

External IDs

OMIM: 609530 MGI: 2659071 HomoloGene: 35477 GeneCards: RAPGEF2 Gene

Gene Ontology
Molecular function	• signal transducer activity • guanyl-nucleotide exchange factor activity • calcium ion binding • Rap guanyl-nucleotide exchange factor activity • diacylglycerol binding • Rap GTPase activator activity
Cellular component	• intracellular • plasma membrane • integral to plasma membrane • membrane
Biological process	• MAPKKK cascade • small GTPase mediated signal transduction • cAMP-mediated signaling • positive regulation of Rap GTPase activity • intracellular signal transduction • regulation of small GTPase mediated signal transduction
Sources: Amigo / QuickGO

Orthologs

Species

Human

Mouse

n/a

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 4:
160.03 – 160.28 Mb

Chr 3:
78.87 – 78.99 Mb

PubMed search

[1]

[2]

Rap guanine nucleotide exchange factor 2 is a protein that in humans is encoded by the RAPGEF2 gene.^[1]^[2]^[3]

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000).[supplied by OMIM]^[3]

Interactions

RAPGEF2 has been shown to interact with RAP1A^[2] and RALGDS.^[4]

References

^ Nagase T, Ishikawa K, Nakajima D, Ohira M, Seki N, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Sep 1997). "Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res 4 (2): 141–50. doi:10.1093/dnares/4.2.141. PMID 9205841.
^ ^a ^b Rebhun JF, Castro AF, Quilliam LA (Nov 2000). "Identification of guanine nucleotide exchange factors (GEFs) for the Rap1 GTPase. Regulation of MR-GEF by M-Ras-GTP interaction". J Biol Chem 275 (45): 34901–8. doi:10.1074/jbc.M005327200. PMID 10934204.
^ ^a ^b "Entrez Gene: RAPGEF2 Rap guanine nucleotide exchange factor (GEF) 2". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9693.
^ Spaargaren, M; Bischoff J R (Dec. 1994). "Identification of the guanine nucleotide dissociation stimulator for Ral as a putative effector molecule of R-ras, H-ras, K-ras, and Rap". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 91 (26): 12609–13. doi:10.1073/pnas.91.26.12609. ISSN 0027-8424. PMC 45488. PMID 7809086. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=45488.

Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction.". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing.". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139146.
Ohtsuka T, Hata Y, Ide N, et al. (1999). "nRap GEP: a novel neural GDP/GTP exchange protein for rap1 small G protein that interacts with synaptic scaffolding molecule (S-SCAM).". Biochem. Biophys. Res. Commun. 265 (1): 38–44. doi:10.1006/bbrc.1999.1619. PMID 10548487.
Liao Y, Kariya K, Hu CD, et al. (2000). "RA-GEF, a novel Rap1A guanine nucleotide exchange factor containing a Ras/Rap1A-associating domain, is conserved between nematode and humans.". J. Biol. Chem. 274 (53): 37815–20. doi:10.1074/jbc.274.53.37815. PMID 10608844.
de Rooij J, Boenink NM, van Triest M, et al. (2000). "PDZ-GEF1, a guanine nucleotide exchange factor specific for Rap1 and Rap2.". J. Biol. Chem. 274 (53): 38125–30. doi:10.1074/jbc.274.53.38125. PMID 10608883.
Chikumi H, Fukuhara S, Gutkind JS (2002). "Regulation of G protein-linked guanine nucleotide exchange factors for Rho, PDZ-RhoGEF, and LARG by tyrosine phosphorylation: evidence of a role for focal adhesion kinase.". J. Biol. Chem. 277 (14): 12463–73. doi:10.1074/jbc.M108504200. PMID 11799111.
Wistow G, Bernstein SL, Wyatt MK, et al. (2002). "Expressed sequence tag analysis of adult human lens for the NEIBank Project: over 2000 non-redundant transcripts, novel genes and splice variants.". Mol. Vis. 8: 171–84. PMID 12107413.
Aurandt J, Vikis HG, Gutkind JS, et al. (2002). "The semaphorin receptor plexin-B1 signals through a direct interaction with the Rho-specific nucleotide exchange factor, LARG.". Proc. Natl. Acad. Sci. U.S.A. 99 (19): 12085–90. doi:10.1073/pnas.142433199. PMC 129402. PMID 12196628. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=129402.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes.". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1356129.
Hisata S, Sakisaka T, Baba T, et al. (2007). "Rap1-PDZ-GEF1 interacts with a neurotrophin receptor at late endosomes, leading to sustained activation of Rap1 and ERK and neurite outgrowth.". J. Cell Biol. 178 (5): 843–60. doi:10.1083/jcb.200610073. PMC 2064548. PMID 17724123. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2064548.

RAPGEF2

Interactions

References

Further reading