RAP2A

RAP2A, member of RAS oncogene family

PDB rendering based on 1kao.

Available structures
PDB	1KAO, 2RAP, 3RAP

Identifiers

Symbols

RAP2A; K-REV; KREV; RAP2; RbBP-30

External IDs

OMIM: 179540 MGI: 97855 HomoloGene: 10875 GeneCards: RAP2A Gene

Gene Ontology
Molecular function	• nucleotide binding • GTPase activity • protein binding • GTP binding
Cellular component	• intracellular • cytosol • membrane • recycling endosome • recycling endosome membrane
Biological process	• GTP catabolic process • signal transduction • small GTPase mediated signal transduction • negative regulation of cell migration • actin cytoskeleton reorganization • positive regulation of protein autophosphorylation • Rap protein signal transduction • cellular protein localization • establishment of protein localization • regulation of JNK cascade • regulation of dendrite morphogenesis
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 13:
98.09 – 98.12 Mb

Chr 14:
120.88 – 120.91 Mb

PubMed search

[1]

[2]

Ras-related protein Rap-2a is a protein that in humans is encoded by the RAP2A gene.^[1] RAP2A is a member of the Ras-related protein family.

Interactions

RAP2A has been shown to interact with RUNDC3A,^[2] RASSF5^[3] and RALGDS.^[4]

References

^ "Entrez Gene: RAP2A RAP2A, member of RAS oncogene family". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5911.
^ Janoueix-Lerosey, I; Pasheva E, de Tand M F, Tavitian A, de Gunzburg J (Mar. 1998). "Identification of a specific effector of the small GTP-binding protein Rap2". Eur. J. Biochem. (GERMANY) 252 (2): 290–8. doi:10.1046/j.1432-1327.1998.2520290.x. ISSN 0014-2956. PMID 9523700.
^ Ortiz-Vega, Sara; Khokhlatchev Andrei, Nedwidek Maria, Zhang Xian-feng, Dammann Reinhard, Pfeifer Gerd P, Avruch Joseph (Feb. 2002). "The putative tumor suppressor RASSF1A homodimerizes and heterodimerizes with the Ras-GTP binding protein Nore1". Oncogene (England) 21 (9): 1381–90. doi:10.1038/sj.onc.1205192. ISSN 0950-9232. PMID 11857081.
^ Nancy, V; Wolthuis R M, de Tand M F, Janoueix-Lerosey I, Bos J L, de Gunzburg J (Mar. 1999). "Identification and characterization of potential effector molecules of the Ras-related GTPase Rap2". J. Biol. Chem. (UNITED STATES) 274 (13): 8737–45. doi:10.1074/jbc.274.13.8737. ISSN 0021-9258. PMID 10085114.

Lerosey I, Chardin P, de Gunzburg J, Tavitian A (1991). "The product of the rap2 gene, member of the ras superfamily. Biochemical characterization and site-directed mutagenesis.". J. Biol. Chem. 266 (7): 4315–21. PMID 1900290.
Pizon V, Chardin P, Lerosey I et al. (1988). "Human cDNAs rap1 and rap2 homologous to the Drosophila gene Dras3 encode proteins closely related to ras in the 'effector' region". Oncogene 3 (2): 201–4. PMID 3045729.
Farrell FX, Yamamoto K, Lapetina EG (1993). "Prenyl group identification of rap2 proteins: a ras superfamily member other than ras that is farnesylated". Biochem. J. 289 ( Pt 2) (Pt 2): 349–55. PMC 1132174. PMID 8424780. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1132174.
Cherfils J, Ménétrey J, Le Bras G et al. (1997). "Crystal structures of the small G protein Rap2A in complex with its substrate GTP, with GDP and with GTPgammaS". EMBO J. 16 (18): 5582–91. doi:10.1093/emboj/16.18.5582. PMC 1170190. PMID 9312017. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1170190.
Janoueix-Lerosey I, Pasheva E, de Tand MF et al. (1998). "Identification of a specific effector of the small GTP-binding protein Rap2". Eur. J. Biochem. 252 (2): 290–8. doi:10.1046/j.1432-1327.1998.2520290.x. PMID 9523700.
Nancy V, Wolthuis RM, de Tand MF et al. (1999). "Identification and characterization of potential effector molecules of the Ras-related GTPase Rap2". J. Biol. Chem. 274 (13): 8737–45. doi:10.1074/jbc.274.13.8737. PMID 10085114.
Linnemann T, Geyer M, Jaitner BK et al. (1999). "Thermodynamic and kinetic characterization of the interaction between the Ras binding domain of AF6 and members of the Ras subfamily". J. Biol. Chem. 274 (19): 13556–62. doi:10.1074/jbc.274.19.13556. PMID 10224125.
Ménétrey J, Cherfils J (2000). "Structure of the small G protein Rap2 in a non-catalytic complex with GTP". Proteins 37 (3): 465–73. doi:10.1002/(SICI)1097-0134(19991115)37:3<465::AID-PROT13>3.0.CO;2-O. PMID 10591105.
de Rooij J, Rehmann H, van Triest M et al. (2000). "Mechanism of regulation of the Epac family of cAMP-dependent RapGEFs". J. Biol. Chem. 275 (27): 20829–36. doi:10.1074/jbc.M001113200. PMID 10777494.
Husi H, Ward MA, Choudhary JS et al. (2000). "Proteomic analysis of NMDA receptor-adhesion protein signaling complexes". Nat. Neurosci. 3 (7): 661–9. doi:10.1038/76615. PMID 10862698.
Traver S, Bidot C, Spassky N et al. (2001). "RGS14 is a novel Rap effector that preferentially regulates the GTPase activity of galphao". Biochem. J. 350 Pt 1 (Pt 1): 19–29. PMC 1221220. PMID 10926822. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1221220.
Gao X, Satoh T, Liao Y et al. (2001). "Identification and characterization of RA-GEF-2, a Rap guanine nucleotide exchange factor that serves as a downstream target of M-Ras". J. Biol. Chem. 276 (45): 42219–25. doi:10.1074/jbc.M105760200. PMID 11524421.
Ortiz-Vega S, Khokhlatchev A, Nedwidek M et al. (2002). "The putative tumor suppressor RASSF1A homodimerizes and heterodimerizes with the Ras-GTP binding protein Nore1". Oncogene 21 (9): 1381–90. doi:10.1038/sj.onc.1205192. PMID 11857081.
Song C, Satoh T, Edamatsu H et al. (2002). "Differential roles of Ras and Rap1 in growth factor-dependent activation of phospholipase C epsilon". Oncogene 21 (53): 8105–13. doi:10.1038/sj.onc.1206003. PMID 12444546.
Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Kuiperij HB, de Rooij J, Rehmann H et al. (2003). "Characterisation of PDZ-GEFs, a family of guanine nucleotide exchange factors specific for Rap1 and Rap2". Biochim. Biophys. Acta 1593 (2–3): 141–9. doi:10.1016/S0167-4889(02)00365-8. PMID 12581858.
Ota T, Suzuki Y, Nishikawa T et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Machida N, Umikawa M, Takei K et al. (2004). "Mitogen-activated protein kinase kinase kinase kinase 4 as a putative effector of Rap2 to activate the c-Jun N-terminal kinase". J. Biol. Chem. 279 (16): 15711–4. doi:10.1074/jbc.C300542200. PMID 14966141.
Mitin NY, Ramocki MB, Zullo AJ et al. (2004). "Identification and characterization of rain, a novel Ras-interacting protein with a unique subcellular localization". J. Biol. Chem. 279 (21): 22353–61. doi:10.1074/jbc.M312867200. PMID 15031288.
Dunham A, Matthews LH, Burton J et al. (2004). "The DNA sequence and analysis of human chromosome 13". Nature 428 (6982): 522–8. doi:10.1038/nature02379. PMC 2665288. PMID 15057823. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2665288.

PDB gallery

1kao: CRYSTAL STRUCTURE OF THE SMALL G PROTEIN RAP2A WITH GDP

2rap: THE SMALL G PROTEIN RAP2A IN COMPLEX WITH GTP

3rap: The small G protein Rap2 in a non catalytic complex with GTP

RAP2A

Interactions

References

Further reading