RAB4A

RAB4A, member RAS oncogene family

PDB rendering based on 1yu9.

Available structures
PDB	1YU9, 1Z0K, 2BMD, 2BME

Identifiers

RAB4A; HRES-1/RAB4; RAB4

External IDs

OMIM: 179511 MGI: 105069 HomoloGene: 101538 GeneCards: RAB4A Gene

Gene Ontology
Molecular function	• nucleotide binding • ATPase activator activity • GTPase activity • protein binding • GTP binding • protein transporter activity • GDP binding • syntaxin binding • ionotropic glutamate receptor binding • ATPase binding
Cellular component	• soluble fraction • insoluble fraction • cytoplasm • endosome • microsome • cytosol • plasma membrane • insulin-responsive compartment
Biological process	• GTP catabolic process • GTP catabolic process • small GTPase mediated signal transduction • regulation of endocytosis • Rab protein signal transduction
Sources: Amigo / QuickGO

Orthologs

Species

Human

Mouse

ENSG00000168118

ENSMUSG00000019478

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 1:
229.41 – 229.44 Mb

Chr 8:
126.33 – 126.36 Mb

PubMed search

Ras-related protein Rab-4A is a protein that in humans is encoded by the RAB4A gene.^[1]

Interactions

RAB4A has been shown to interact with STX4,^[2] RAB11FIP1,^[3] RABEP1,^[4] CD2AP^[5] and KIF3B.^[6]

References

^ "Entrez Gene: RAB4A RAB4A, member RAS oncogene family". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5867.
^ Li, L; Omata W, Kojima I, Shibata H (Feb. 2001). "Direct interaction of Rab4 with syntaxin 4". J. Biol. Chem. (United States) 276 (7): 5265–73. doi:10.1074/jbc.M003883200. ISSN 0021-9258. PMID 11063739.
^ Lindsay, Andrew J; Hendrick Alan G, Cantalupo Giuseppina, Senic-Matuglia Francesca, Goud Bruno, Bucci Cecilia, McCaffrey Mary W (Apr. 2002). "Rab coupling protein (RCP), a novel Rab4 and Rab11 effector protein". J. Biol. Chem. (United States) 277 (14): 12190–9. doi:10.1074/jbc.M108665200. ISSN 0021-9258. PMID 11786538.
^ Vitale, G; Rybin V, Christoforidis S, Thornqvist P, McCaffrey M, Stenmark H, Zerial M (Apr. 1998). "Distinct Rab-binding domains mediate the interaction of Rabaptin-5 with GTP-bound Rab4 and Rab5". EMBO J. (ENGLAND) 17 (7): 1941–51. doi:10.1093/emboj/17.7.1941. ISSN 0261-4189. PMC 1170540. PMID 9524117. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1170540.
^ Cormont, Mireille; Metón Isidoro, Mari Muriel, Monzo Pascale, Keslair Frédérique, Gaskin Chantell, McGraw Timothy E, Le Marchand-Brustel Yannick (Feb. 2003). "CD2AP/CMS regulates endosome morphology and traffic to the degradative pathway through its interaction with Rab4 and c-Cbl". Traffic (Denmark) 4 (2): 97–112. doi:10.1034/j.1600-0854.2003.40205.x. ISSN 1398-9219. PMID 12559036.
^ Imamura, Takeshi; Huang Jie, Usui Isao, Satoh Hiroaki, Bever Jennie, Olefsky Jerrold M (Jul. 2003). "Insulin-induced GLUT4 translocation involves protein kinase C-lambda-mediated functional coupling between Rab4 and the motor protein kinesin". Mol. Cell. Biol. (United States) 23 (14): 4892–900. doi:10.1128/MCB.23.14.4892-4900.2003. ISSN 0270-7306. PMC 162221. PMID 12832475. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=162221.

Further reading

Stibbs HH, Seed JR (1975). "Short-term metabolism of (14-C) tryptophan in rats infected with Trypanosoma brucei gambiense.". J. Infect. Dis. 131 (4): 459–62. PMID 1117200.
van der Sluijs P, Hull M, Huber LA, et al. (1992). "Reversible phosphorylation--dephosphorylation determines the localization of rab4 during the cell cycle.". EMBO J. 11 (12): 4379–89. PMC 557012. PMID 1425574. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=557012.
Bailly E, McCaffrey M, Touchot N, et al. (1991). "Phosphorylation of two small GTP-binding proteins of the Rab family by p34cdc2.". Nature 350 (6320): 715–8. doi:10.1038/350715a0. PMID 1902553.
Rousseau-Merck MF, Zahraoui A, Touchot N, et al. (1991). "Chromosome assignment of four RAS-related RAB genes.". Hum. Genet. 86 (4): 350–4. doi:10.1007/BF00201831. PMID 1999336.
Zahraoui A, Touchot N, Chardin P, Tavitian A (1989). "The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion.". J. Biol. Chem. 264 (21): 12394–401. PMID 2501306.
Cormont M, Tanti JF, Zahraoui A, et al. (1994). "Rab4 is phosphorylated by the insulin-activated extracellular-signal-regulated kinase ERK1.". Eur. J. Biochem. 219 (3): 1081–5. doi:10.1111/j.1432-1033.1994.tb18591.x. PMID 8112321.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Karniguian A, Zahraoui A, Tavitian A (1993). "Identification of small GTP-binding rab proteins in human platelets: thrombin-induced phosphorylation of rab3B, rab6, and rab8 proteins.". Proc. Natl. Acad. Sci. U.S.A. 90 (16): 7647–51. doi:10.1073/pnas.90.16.7647. PMC 47199. PMID 8356066. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=47199.
Valentijn JA, LaCivita DQ, Gumkowski FD, Jamieson JD (1997). "Rab4 associates with the actin terminal web in developing rat pancreatic acinar cells.". Eur. J. Cell Biol. 72 (1): 1–8. PMID 9013719.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Vitale G, Rybin V, Christoforidis S, et al. (1998). "Distinct Rab-binding domains mediate the interaction of Rabaptin-5 with GTP-bound Rab4 and Rab5.". EMBO J. 17 (7): 1941–51. doi:10.1093/emboj/17.7.1941. PMC 1170540. PMID 9524117. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1170540.
Bucci C, Chiariello M, Lattero D, et al. (1999). "Interaction cloning and characterization of the cDNA encoding the human prenylated rab acceptor (PRA1).". Biochem. Biophys. Res. Commun. 258 (3): 657–62. doi:10.1006/bbrc.1999.0651. PMID 10329441.
Shisheva A, Chinni SR, DeMarco C (1999). "General role of GDP dissociation inhibitor 2 in membrane release of Rab proteins: modulations of its functional interactions by in vitro and in vivo structural modifications.". Biochemistry 38 (36): 11711–21. doi:10.1021/bi990200r. PMID 10512627.
Nagelkerken B, Van Anken E, Van Raak M, et al. (2000). "Rabaptin4, a novel effector of the small GTPase rab4a, is recruited to perinuclear recycling vesicles.". Biochem. J. 346 Pt 3 (3): 593–601. doi:10.1042/0264-6021:3460593. PMC 1220890. PMID 10698684. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1220890.
Gerez L, Mohrmann K, van Raak M, et al. (2000). "Accumulation of rab4GTP in the cytoplasm and association with the peptidyl-prolyl isomerase pin1 during mitosis.". Mol. Biol. Cell 11 (7): 2201–11. PMC 14913. PMID 10888662. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=14913.
Li L, Omata W, Kojima I, Shibata H (2001). "Direct interaction of Rab4 with syntaxin 4.". J. Biol. Chem. 276 (7): 5265–73. doi:10.1074/jbc.M003883200. PMID 11063739.
Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination.". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=310948.
Cormont M, Mari M, Galmiche A, et al. (2001). "A FYVE-finger-containing protein, Rabip4, is a Rab4 effector involved in early endosomal traffic.". Proc. Natl. Acad. Sci. U.S.A. 98 (4): 1637–42. doi:10.1073/pnas.031586998. PMC 29309. PMID 11172003. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=29309.
Bielli A, Thörnqvist PO, Hendrick AG, et al. (2001). "The small GTPase Rab4A interacts with the central region of cytoplasmic dynein light intermediate chain-1.". Biochem. Biophys. Res. Commun. 281 (5): 1141–53. doi:10.1006/bbrc.2001.4468. PMID 11243854.
Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing.". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1083732.

PDB gallery

1yu9: GppNHp-Bound Rab4A

1z0k: Structure of GTP-Bound Rab4Q67L GTPase in complex with the central Rab binding domain of Rabenosyn-5

2bmd: HIGH RESOLUTION STRUCTURE OF GDP-BOUND HUMAN RAB4A

2bme: HIGH RESOLUTION STRUCTURE OF GPPNHP-BOUND HUMAN RAB4A