PstI

PstI, is a Type II restriction endonuclease (or restriction enzyme) from Providencia stuartii.[1][2] PstI recognition and cut site are as follows:

Recognition Sequence Cut Site
 5'CTGCAG 3'
 3'GACGTC 5'
 5'--CTGCA  G--3'
 3'--G  ACGTC--5'

References