PCNT
Pericentrin (kendrin), also known as PCNT, is a protein which in humans is encoded by the PCNT gene.[1][2][3]
Function
The protein encoded by this gene binds to calmodulin and is expressed in the centrosome. It is an integral component of the pericentriolar material (PCM). The protein contains a series of coiled coil domains and a highly conserved PCM targeting motif called the PACT domain near its C-terminus[4]. The protein interacts with the microtubule nucleation component γ-tubulin and is likely important to normal functioning of the centrosomes, cytoskeleton, and cell cycle progression.[1]
Interactions
PCNT has been shown to interact with PCM1.[5]
References
- ^ a b "Entrez Gene: PCNT pericentrin (kendrin)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5116.
- ^ Chen H, Gos A, Morris MA, Antonarakis SE (August 1996). "Localization of a human homolog of the mouse pericentrin gene (PCNT) to chromosome 21qter". Genomics 35 (3): 620–4. doi:10.1006/geno.1996.0411. PMID 8812505.
- ^ Flory MR, Moser MJ, Monnat RJ, Davis TN (May 2000). "Identification of a human centrosomal calmodulin-binding protein that shares homology with pericentrin". Proceedings of the National Academy of Sciences of the United States of America 97 (11): 5919–23. doi:10.1073/pnas.97.11.5919. PMC 18534. PMID 10823944. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=18534.
- ^ Gillingham, A.; Munro, S. (2000). "The PACT domain, a conserved centrosomal targeting motif in the coiled-coil proteins AKAP450 and pericentrin". EMBO reports 1 (6): 524–529. doi:10.1093/embo-reports/kvd105. PMC 1083777. PMID 11263498. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1083777. edit
- ^ Li, Q; Hansen D, Killilea A, Joshi H C, Palazzo R E, Balczon R (Feb. 2001). "Kendrin/pericentrin-B, a centrosome protein with homology to pericentrin that complexes with PCM-1". J. Cell. Sci. (England) 114 (Pt 4): 797–809. ISSN 0021-9533. PMID 11171385.
Further reading
- Nakajima D, Okazaki N, Yamakawa H, et al. (2003). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Res. 9 (3): 99–106. doi:10.1093/dnares/9.3.99. PMID 12168954.
- Chen H, Gos A, Morris MA, Antonarakis SE (1996). "Localization of a human homolog of the mouse pericentrin gene (PCNT) to chromosome 21qter". Genomics 35 (3): 620–4. doi:10.1006/geno.1996.0411. PMID 8812505.
- Ishikawa K, Nagase T, Nakajima D, et al. (1998). "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 4 (5): 307–13. doi:10.1093/dnares/4.5.307. PMID 9455477.
- Diviani D, Langeberg LK, Doxsey SJ, Scott JD (2000). "Pericentrin anchors protein kinase A at the centrosome through a newly identified RII-binding domain". Curr. Biol. 10 (7): 417–20. doi:10.1016/S0960-9822(00)00422-X. PMID 10753751.
- Flory MR, Moser MJ, Monnat RJ, Davis TN (2000). "Identification of a human centrosomal calmodulin-binding protein that shares homology with pericentrin". Proc. Natl. Acad. Sci. U.S.A. 97 (11): 5919–23. doi:10.1073/pnas.97.11.5919. PMC 18534. PMID 10823944. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=18534.
- Hattori M, Fujiyama A, Taylor TD, et al. (2000). "The DNA sequence of human chromosome 21". Nature 405 (6784): 311–9. doi:10.1038/35012518. PMID 10830953.
- Li Q, Hansen D, Killilea A, et al. (2001). "Kendrin/pericentrin-B, a centrosome protein with homology to pericentrin that complexes with PCM-1". J. Cell. Sci. 114 (Pt 4): 797–809. PMID 11171385.
- Takahashi M, Yamagiwa A, Nishimura T, et al. (2003). "Centrosomal Proteins CG-NAP and Kendrin Provide Microtubule Nucleation Sites by Anchoring γ-Tubulin Ring Complex". Mol. Biol. Cell 13 (9): 3235–45. doi:10.1091/mbc.E02-02-0112. PMC 124155. PMID 12221128. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=124155.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
- Flory MR, Davis TN (2004). "The centrosomal proteins pericentrin and kendrin are encoded by alternatively spliced products of one gene". Genomics 82 (3): 401–5. doi:10.1016/S0888-7543(03)00119-8. PMID 12906865.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Chang F, Re F, Sebastian S, et al. (2004). "HIV-1 Vpr Induces Defects in Mitosis, Cytokinesis, Nuclear Structure, and Centrosomes". Mol. Biol. Cell 15 (4): 1793–801. doi:10.1091/mbc.E03-09-0691. PMC 379276. PMID 14767062. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=379276.
- Miyoshi K, Asanuma M, Miyazaki I, et al. (2004). "DISC1 localizes to the centrosome by binding to kendrin". Biochem. Biophys. Res. Commun. 317 (4): 1195–9. doi:10.1016/j.bbrc.2004.03.163. PMID 15094396.
- Jurczyk A, Gromley A, Redick S, et al. (2004). "Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assembly". J. Cell Biol. 166 (5): 637–43. doi:10.1083/jcb.200405023. PMC 2172416. PMID 15337773. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2172416.
- Suzuki Y, Yamashita R, Shirota M, et al. (2004). "Sequence Comparison of Human and Mouse Genes Reveals a Homologous Block Structure in the Promoter Regions". Genome Res. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC 515316. PMID 15342556. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=515316.
- Golubkov VS, Chekanov AV, Doxsey SJ, Strongin AY (2006). "Centrosomal pericentrin is a direct cleavage target of membrane type-1 matrix metalloproteinase in humans but not in mice: potential implications for tumorigenesis". J. Biol. Chem. 280 (51): 42237–41. doi:10.1074/jbc.M510139200. PMID 16251193.
- Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1459365.