PRC1
Protein regulator of cytokinesis 1 is a protein that in humans is encoded by the PRC1 gene.[1][2]
This gene encodes a protein that is involved in cytokinesis. The encoded protein is at high level during S and G2/M and drop dramatically after cell exit mitosis and enter G1. It is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). At least three alternatively spliced transcript variants encoding distinct isoforms have been observed.[2]
Interactions
PRC1 has been shown to interact with TRIM37.[3]
References
- ^ Jiang W, Jimenez G, Wells NJ, Hope TJ, Wahl GM, Hunter T, Fukunaga R (Jan 1999). "PRC1: a human mitotic spindle-associated CDK substrate protein required for cytokinesis". Mol Cell 2 (6): 877–85. doi:10.1016/S1097-2765(00)80302-0. PMID 9885575.
- ^ a b "Entrez Gene: PRC1 protein regulator of cytokinesis 1". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9055.
- ^ Rual, Jean-François; Venkatesan Kavitha, Hao Tong, Hirozane-Kishikawa Tomoko, Dricot Amélie, Li Ning, Berriz Gabriel F, Gibbons Francis D, Dreze Matija, Ayivi-Guedehoussou Nono, Klitgord Niels, Simon Christophe, Boxem Mike, Milstein Stuart, Rosenberg Jennifer, Goldberg Debra S, Zhang Lan V, Wong Sharyl L, Franklin Giovanni, Li Siming, Albala Joanna S, Lim Janghoo, Fraughton Carlene, Llamosas Estelle, Cevik Sebiha, Bex Camille, Lamesch Philippe, Sikorski Robert S, Vandenhaute Jean, Zoghbi Huda Y, Smolyar Alex, Bosak Stephanie, Sequerra Reynaldo, Doucette-Stamm Lynn, Cusick Michael E, Hill David E, Roth Frederick P, Vidal Marc (Oct. 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature (England) 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
Further reading
- Mollinari C, Kleman JP, Jiang W, et al. (2002). "PRC1 is a microtubule binding and bundling protein essential to maintain the mitotic spindle midzone.". J. Cell Biol. 157 (7): 1175–86. doi:10.1083/jcb.200111052. PMC 2173564. PMID 12082078. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2173564.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
- Ban R, Irino Y, Fukami K, Tanaka H (2004). "Human mitotic spindle-associated protein PRC1 inhibits MgcRacGAP activity toward Cdc42 during the metaphase.". J. Biol. Chem. 279 (16): 16394–402. doi:10.1074/jbc.M313257200. PMID 14744859.
- Kurasawa Y, Earnshaw WC, Mochizuki Y, et al. (2005). "Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation.". EMBO J. 23 (16): 3237–48. doi:10.1038/sj.emboj.7600347. PMC 514520. PMID 15297875. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=514520.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
- Li C, Lin M, Liu J (2005). "Identification of PRC1 as the p53 target gene uncovers a novel function of p53 in the regulation of cytokinesis.". Oncogene 23 (58): 9336–47. doi:10.1038/sj.onc.1208114. PMID 15531928.
- Mollinari C, Kleman JP, Saoudi Y, et al. (2005). "Ablation of PRC1 by small interfering RNA demonstrates that cytokinetic abscission requires a central spindle bundle in mammalian cells, whereas completion of furrowing does not.". Mol. Biol. Cell 16 (3): 1043–55. doi:10.1091/mbc.E04-04-0346. PMC 551472. PMID 15616196. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=551472.
- Zhu C, Jiang W (2005). "Cell cycle-dependent translocation of PRC1 on the spindle by Kif4 is essential for midzone formation and cytokinesis.". Proc. Natl. Acad. Sci. U.S.A. 102 (2): 343–8. doi:10.1073/pnas.0408438102. PMC 544298. PMID 15625105. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=544298.
- Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network.". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
- Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle.". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1459365.
- Zhu C, Lau E, Schwarzenbacher R, et al. (2006). "Spatiotemporal control of spindle midzone formation by PRC1 in human cells.". Proc. Natl. Acad. Sci. U.S.A. 103 (16): 6196–201. doi:10.1073/pnas.0506926103. PMC 1458854. PMID 16603632. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1458854.
- Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization.". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243.
- Shimo A, Nishidate T, Ohta T, et al. (2007). "Elevated expression of protein regulator of cytokinesis 1, involved in the growth of breast cancer cells.". Cancer Sci. 98 (2): 174–81. doi:10.1111/j.1349-7006.2006.00381.x. PMID 17233835.