POU2AF1

POU class 2 associating factor 1

PDB rendering based on 1cqt.

Available structures
PDB	1cqt

Identifiers

POU2AF1; BOB1; OBF-1; OBF1; OCAB

External IDs

OMIM: 601206 MGI: 105086 HomoloGene: 4543 GeneCards: POU2AF1 Gene

Gene Ontology
Molecular function	• DNA binding • transcription cofactor activity • transcription coactivator activity • protein binding
Cellular component	• nucleus
Biological process	• regulation of transcription, DNA-dependent • transcription from RNA polymerase II promoter • humoral immune response
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

ENSG00000110777

ENSMUSG00000032053

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 11:
111.22 – 111.33 Mb

Chr 9:
51.02 – 51.05 Mb

PubMed search

POU domain class 2-associating factor 1 is a protein that in humans is encoded by the POU2AF1 gene.^[1]^[2]

Interactions

POU2AF1 has been shown to interact with POU2F1^[3]^[4]^[5] and SIAH1.^[6]

References

^ Junker S, Brondum-Nielsen K, Newell JW, Matthias P, Tommerup N (Jun 1996). "Assignment of the human gene for Oct-binding factor-1 (OBF1), a B-cell-specific coactivator of octamer-binding transcription factors 1 and 2, to 11q23.1 by somatic cell hybridization and in situ hybridization". Genomics 33 (1): 143–5. doi:10.1006/geno.1996.0173. PMID 8617501.
^ "Entrez Gene: POU2AF1 POU domain, class 2, associating factor 1". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5450.
^ Chasman, D; Cepek K, Sharp P A, Pabo C O (Oct. 1999). "Crystal structure of an OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex: specific recognition of a protein-DNA interface". Genes Dev. (UNITED STATES) 13 (20): 2650–7. doi:10.1101/gad.13.20.2650. ISSN 0890-9369. PMC 317104. PMID 10541551. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=317104.
^ Wong, M W; Henry R W, Ma B, Kobayashi R, Klages N, Matthias P, Strubin M, Hernandez N (Jan. 1998). "The Large Subunit of Basal Transcription Factor SNAPc Is a Myb Domain Protein That Interacts with Oct-1". Mol. Cell. Biol. (UNITED STATES) 18 (1): 368–77. ISSN 0270-7306. PMC 121507. PMID 9418884. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=121507.
^ Lee, L; Stollar E, Chang J, Grossmann J G, O'Brien R, Ladbury J, Carpenter B, Roberts S, Luisi B (Jun. 2001). "Expression of the Oct-1 transcription factor and characterization of its interactions with the Bob1 coactivator". Biochemistry (United States) 40 (22): 6580–8. doi:10.1021/bi010095x. ISSN 0006-2960. PMID 11380252.
^ Tiedt, R; Bartholdy B A, Matthias G, Newell J W, Matthias P (Aug. 2001). "The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1". EMBO J. (England) 20 (15): 4143–52. doi:10.1093/emboj/20.15.4143. ISSN 0261-4189. PMC 149178. PMID 11483517. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=149178.

Further reading

Teitell MA (2004). "OCA-B regulation of B-cell development and function". Trends Immunol. 24 (10): 546–53. doi:10.1016/j.it.2003.08.002. PMID 14552839.
Luo Y, Roeder RG (1995). "Cloning, functional characterization, and mechanism of action of the B-cell-specific transcriptional coactivator OCA-B". Mol. Cell. Biol. 15 (8): 4115–24. PMC 230650. PMID 7623806. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=230650.
Gstaiger M, Knoepfel L, Georgiev O et al. (1995). "A B-cell coactivator of octamer-binding transcription factors". Nature 373 (6512): 360–2. doi:10.1038/373360a0. PMID 7779176.
Strubin M, Newell JW, Matthias P (1995). "OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins". Cell 80 (3): 497–506. doi:10.1016/0092-8674(95)90500-6. PMID 7859290.
Galiègue-Zouitina S, Quief S, Hildebrand MP et al. (1996). "Fusion of the LAZ3/BCL6 and BOB1/OBF1 genes by t(3; 11) (q27; q23) chromosomal translocation". C. R. Acad. Sci. III, Sci. Vie 318 (11): 1125–31. PMID 8574789.
Zwilling S, Dieckmann A, Pfisterer P et al. (1997). "Inducible expression and phosphorylation of coactivator BOB.1/OBF.1 in T cells". Science 277 (5323): 221–5. doi:10.1126/science.277.5323.221. PMID 9211847.
Sauter P, Matthias P (1998). "The B cell-specific coactivator OBF-1 (OCA-B, Bob-1) is inducible in T cells and its expression is dispensable for IL-2 gene induction". Immunobiology 198 (1–3): 207–16. PMID 9442392.
Luo Y, Ge H, Stevens S et al. (1998). "Coactivation by OCA-B: Definition of Critical Regions and Synergism with General Cofactors". Mol. Cell. Biol. 18 (7): 3803–10. PMC 108964. PMID 9632764. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=108964.
Sauter P, Matthias P (1998). "Coactivator OBF-1 Makes Selective Contacts with Both the POU-Specific Domain and the POU Homeodomain and Acts as a Molecular Clamp on DNA". Mol. Cell. Biol. 18 (12): 7397–409. PMC 109321. PMID 9819426. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=109321.
Chasman D, Cepek K, Sharp PA, Pabo CO (1999). "Crystal structure of an OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex: specific recognition of a protein-DNA interface". Genes Dev. 13 (20): 2650–7. doi:10.1101/gad.13.20.2650. PMC 317104. PMID 10541551. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=317104.
Tomilin A, Reményi A, Lins K et al. (2001). "Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration". Cell 103 (6): 853–64. doi:10.1016/S0092-8674(00)00189-6. PMID 11136971.
Lee L, Stollar E, Chang J et al. (2001). "Expression of the Oct-1 transcription factor and characterization of its interactions with the Bob1 coactivator". Biochemistry 40 (22): 6580–8. doi:10.1021/bi010095x. PMID 11380252.
Tiedt R, Bartholdy BA, Matthias G et al. (2001). "The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1". EMBO J. 20 (15): 4143–52. doi:10.1093/emboj/20.15.4143. PMC 149178. PMID 11483517. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=149178.
Boehm J, He Y, Greiner A et al. (2001). "Regulation of BOB.1/OBF.1 stability by SIAH". EMBO J. 20 (15): 4153–62. doi:10.1093/emboj/20.15.4153. PMC 149152. PMID 11483518. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=149152.
Malone CS, Wall R (2002). "Bob1 (OCA-B/OBF-1) differential transactivation of the B cell-specific B29 (Ig beta) and mb-1 (Ig alpha) promoters". J. Immunol. 168 (7): 3369–75. PMID 11907094.
Casellas R, Jankovic M, Meyer G et al. (2003). "OcaB is required for normal transcription and V(D)J recombination of a subset of immunoglobulin kappa genes". Cell 110 (5): 575–85. doi:10.1016/S0092-8674(02)00911-X. PMID 12230975.
Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Schaffer A, Kim EC, Wu X et al. (2003). "Selective inhibition of class switching to IgG and IgE by recruitment of the HoxC4 and Oct-1 homeodomain proteins and Ku70/Ku86 to newly identified ATTT cis-elements". J. Biol. Chem. 278 (25): 23141–50. doi:10.1074/jbc.M212952200. PMID 12672812.
Lins K, Reményi A, Tomilin A et al. (2003). "OBF1 enhances transcriptional potential of Oct1". EMBO J. 22 (9): 2188–98. doi:10.1093/emboj/cdg199. PMC 156071. PMID 12727885. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=156071.

PDB gallery

1cqt: CRYSTAL STRUCTURE OF A TERNARY COMPLEX CONTAINING AN OCA-B PEPTIDE, THE OCT-1 POU DOMAIN, AND AN OCTAMER ELEMENT