PNKP

Polynucleotide kinase 3'-phosphatase

PDB rendering based on 2brf.

Available structures
PDB	2BRF, 2W3O

Identifiers

Symbols

PNKP; EIEE10; MCSZ; PNK

External IDs

OMIM: 605610 MGI: 1891698 HomoloGene: 5247 GeneCards: PNKP Gene

Gene Ontology
Molecular function	• damaged DNA binding • double-stranded DNA binding • endonuclease activity • protein binding • ATP binding • kinase activity • transferase activity • hydrolase activity • purine nucleotide binding • nucleotide kinase activity • polynucleotide 3'-phosphatase activity • ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity
Cellular component	• nucleus • nucleolus • nucleolus
Biological process	• nucleotide-excision repair, DNA damage removal • DNA-dependent DNA replication • DNA repair • response to oxidative stress • response to radiation • response to radiation • dephosphorylation • DNA damage response, detection of DNA damage • DNA damage response, detection of DNA damage • nucleotide phosphorylation
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 19:
50.36 – 50.37 Mb

Chr 7:
52.11 – 52.12 Mb

PubMed search

[1]

[2]

Bifunctional polynucleotide phosphatase/kinase is an enzyme that in humans is encoded by the PNKP gene.^[1]^[2]^[3]

Interactions

PNKP has been shown to interact with DNA polymerase beta^[4] and XRCC1.^[4]^[5]

References

^ Jilani A, Ramotar D, Slack C, Ong C, Yang XM, Scherer SW, Lasko DD (September 1999). "Molecular cloning of the human gene, PNKP, encoding a polynucleotide kinase 3'-phosphatase and evidence for its role in repair of DNA strand breaks caused by oxidative damage". J Biol Chem 274 (34): 24176–24186. doi:10.1074/jbc.274.34.24176. PMID 10446192.
^ Karimi-Busheri F, Daly G, Robins P, Canas B, Pappin DJ, Sgouros J, Miller GG, Fakhrai H, Davis EM, Le Beau MM, Weinfeld M (September 1999). "Molecular characterization of a human DNA kinase". J Biol Chem 274 (34): 24187–24194. doi:10.1074/jbc.274.34.24187. PMID 10446193.
^ "Entrez Gene: PNKP polynucleotide kinase 3'-phosphatase". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=11284.
^ ^a ^b Whitehouse, C J; Taylor R M, Thistlethwaite A, Zhang H, Karimi-Busheri F, Lasko D D, Weinfeld M, Caldecott K W (January2001). "XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair". Cell (United States) 104 (1): 107–117. doi:10.1016/S0092-8674(01)00195-7. ISSN 0092-8674. PMID 11163244.
^ Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1847948.

Whitehouse CJ, Taylor RM, Thistlethwaite A et al. (2001). "XRCC1 stimulates human polynucleotide kinase activity at damaged DNA termini and accelerates DNA single-strand break repair". Cell 104 (1): 107–117. doi:10.1016/S0092-8674(01)00195-7. PMID 11163244.
Chappell C, Hanakahi LA, Karimi-Busheri F et al. (2002). "Involvement of human polynucleotide kinase in double-strand break repair by non-homologous end joining". EMBO J. 21 (11): 2827–2832. doi:10.1093/emboj/21.11.2827. PMC 126026. PMID 12032095. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=126026.
Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–16903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Mani RS, Karimi-Busheri F, Fanta M et al. (2004). "Spectroscopic studies of DNA and ATP binding to human polynucleotide kinase: evidence for a ternary complex". Biochemistry 42 (41): 12077–12084. doi:10.1021/bi030127b. PMID 14556639.
Grimwood J, Gordon LA, Olsen A et al. (2004). "The DNA sequence and biology of human chromosome 19". Nature 428 (6982): 529–535. doi:10.1038/nature02399. PMID 15057824.
Loizou JI, El-Khamisy SF, Zlatanou A et al. (2004). "The protein kinase CK2 facilitates repair of chromosomal DNA single-strand breaks". Cell 117 (1): 17–28. doi:10.1016/S0092-8674(04)00206-5. PMID 15066279.
Colland F, Jacq X, Trouplin V et al. (2004). "Functional proteomics mapping of a human signaling pathway". Genome Res. 14 (7): 1324–1332. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=442148.
Wiederhold L, Leppard JB, Kedar P et al. (2004). "AP endonuclease-independent DNA base excision repair in human cells". Mol. Cell 15 (2): 209–220. doi:10.1016/j.molcel.2004.06.003. PMID 15260972.
Koch CA, Agyei R, Galicia S et al. (2005). "Xrcc4 physically links DNA end processing by polynucleotide kinase to DNA ligation by DNA ligase IV". EMBO J. 23 (19): 3874–3885. doi:10.1038/sj.emboj.7600375. PMC 522785. PMID 15385968. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=522785.
Gerhard DS, Wagner L, Feingold EA et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–2127. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Andersen JS, Lam YW, Leung AK et al. (2005). "Nucleolar proteome dynamics". Nature 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413.
Rual JF, Venkatesan K, Hao T et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–1178. doi:10.1038/nature04209. PMID 16189514.
Audebert M, Salles B, Weinfeld M, Calsou P (2006). "Involvement of polynucleotide kinase in a poly(ADP-ribose) polymerase-1-dependent DNA double-strand breaks rejoining pathway". J. Mol. Biol. 356 (2): 257–265. doi:10.1016/j.jmb.2005.11.028. PMID 16364363.
Olsen JV, Blagoev B, Gnad F et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell 127 (3): 635–648. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
Ewing RM, Chu P, Elisma F et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1847948.
Karimi-Busheri F, Rasouli-Nia A, Allalunis-Turner J, Weinfeld M (2007). "Human polynucleotide kinase participates in repair of DNA double-strand breaks by nonhomologous end joining but not homologous recombination". Cancer Res. 67 (14): 6619–6625. doi:10.1158/0008-5472.CAN-07-0480. PMID 17638872.
Mani RS, Fanta M, Karimi-Busheri F et al. (2007). "XRCC1 stimulates polynucleotide kinase by enhancing its damage discrimination and displacement from DNA repair intermediates". J. Biol. Chem. 282 (38): 28004–28013. doi:10.1074/jbc.M704867200. PMID 17650498.

PDB gallery

2brf: CRYSTAL STRUCTURE OF THE FHA DOMAIN OF HUMAN POLYNUCLEOTIDE KINASE 3' PHOSPHATASE

PNKP

Interactions

References

Further reading