PLCE1

Phospholipase C, epsilon 1

PDB rendering based on 2bye.

Available structures
PDB	2BYE, 2BYF, 2C5L

Identifiers

Symbols

PLCE1; FLJ23659; KIAA1516; MGC167842; NPHS3; PLCE; PPLC

External IDs

OMIM: 608414 MGI: 1921305 HomoloGene: 9478 GeneCards: PLCE1 Gene

EC number

3.1.4.11

Gene Ontology
Molecular function	• phosphatidylinositol phospholipase C activity • phospholipase C activity • receptor signaling protein activity • guanyl-nucleotide exchange factor activity • calcium ion binding • protein binding • hydrolase activity • Ras GTPase binding • enzyme binding
Cellular component	• Golgi membrane • membrane fraction • cytoplasm • Golgi apparatus • cytosol • plasma membrane
Biological process	• activation of MAPK activity • regulation of cell growth • phospholipid metabolic process • diacylglycerol biosynthetic process • regulation of smooth muscle contraction • cytoskeleton organization • epidermal growth factor receptor signaling pathway • activation of phospholipase C activity by G-protein coupled receptor protein signaling pathway coupled to IP3 second messenger • elevation of cytosolic calcium ion concentration • activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway • Ras protein signal transduction • heart development • regulation of G-protein coupled receptor protein signaling pathway • cell proliferation • lipid catabolic process • calcium-mediated signaling • calcium-mediated signaling • glomerulus development • regulation of protein kinase activity • regulation of Ras protein signal transduction • inositol phosphate-mediated signaling
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 10:
95.75 – 96.09 Mb

Chr 19:
38.6 – 38.86 Mb

PubMed search

[1]

[2]

1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase epsilon-1 is an enzyme that in humans is encoded by the PLCE1 gene.^[1]^[2]^[3]

Function

PLCE1 belongs to the phospholipase family that catalyzes the hydrolysis of polyphosphoinositides such as phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2) to generate the second messengers Ins(1,4,5)P3 and diacylglycerol. These products initiate a cascade of intracellular responses that result in cell growth and differentiation and gene expression.[supplied by OMIM]^[3]

References

^ Lopez I, Mak EC, Ding J, Hamm HE, Lomasney JW (May 2001). "A novel bifunctional phospholipase c that is regulated by Galpha 12 and stimulates the Ras/mitogen-activated protein kinase pathway". J Biol Chem 276 (4): 2758–65. doi:10.1074/jbc.M008119200. PMID 11022047.
^ Song C, Hu CD, Masago M, Kariyai K, Yamawaki-Kataoka Y, Shibatohge M, Wu D, Satoh T, Kataoka T (May 2001). "Regulation of a novel human phospholipase C, PLCepsilon, through membrane targeting by Ras". J Biol Chem 276 (4): 2752–7. doi:10.1074/jbc.M008324200. PMID 11022048.
^ ^a ^b "Entrez Gene: PLCE1 phospholipase C, epsilon 1". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=51196.

Jefferson JA, Shankland SJ (2007). "Familial nephrotic syndrome: PLCE1 enters the fray.". Nephrol. Dial. Transplant. 22 (7): 1849–52. doi:10.1093/ndt/gfm098. PMID 17449496.
Cefai D, Debre P, Kaczorek M, et al. (1991). "Human immunodeficiency virus-1 glycoproteins gp120 and gp160 specifically inhibit the CD3/T cell-antigen receptor phosphoinositide transduction pathway.". J. Clin. Invest. 86 (6): 2117–24. doi:10.1172/JCI114950. PMC 329852. PMID 1979339. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=329852.
Zauli G, Previati M, Caramelli E, et al. (1995). "Exogenous human immunodeficiency virus type-1 Tat protein selectively stimulates a phosphatidylinositol-specific phospholipase C nuclear pathway in the Jurkat T cell line.". Eur. J. Immunol. 25 (9): 2695–700. doi:10.1002/eji.1830250944. PMID 7589147.
Chen P, Mayne M, Power C, Nath A (1997). "The Tat protein of HIV-1 induces tumor necrosis factor-alpha production. Implications for HIV-1-associated neurological diseases.". J. Biol. Chem. 272 (36): 22385–8. doi:10.1074/jbc.272.36.22385. PMID 9278385.
Mayne M, Bratanich AC, Chen P, et al. (1998). "HIV-1 tat molecular diversity and induction of TNF-alpha: implications for HIV-induced neurological disease.". Neuroimmunomodulation 5 (3-4): 184–92. doi:10.1159/000026336. PMID 9730685.
Haughey NJ, Holden CP, Nath A, Geiger JD (1999). "Involvement of inositol 1,4,5-trisphosphate-regulated stores of intracellular calcium in calcium dysregulation and neuron cell death caused by HIV-1 protein tat.". J. Neurochem. 73 (4): 1363–74. doi:10.1046/j.1471-4159.1999.0731363.x. PMID 10501179.
Nagase T, Kikuno R, Ishikawa K, et al. (2000). "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.". DNA Res. 7 (2): 143–50. doi:10.1093/dnares/7.2.143. PMID 10819331.
Mayne M, Holden CP, Nath A, Geiger JD (2000). "Release of calcium from inositol 1,4,5-trisphosphate receptor-regulated stores by HIV-1 Tat regulates TNF-alpha production in human macrophages.". J. Immunol. 164 (12): 6538–42. PMID 10843712.
Jin TG, Satoh T, Liao Y, et al. (2001). "Role of the CDC25 homology domain of phospholipase Cepsilon in amplification of Rap1-dependent signaling.". J. Biol. Chem. 276 (32): 30301–7. doi:10.1074/jbc.M103530200. PMID 11395506.
Schmidt M, Evellin S, Weernink PA, et al. (2001). "A new phospholipase-C-calcium signalling pathway mediated by cyclic AMP and a Rap GTPase.". Nat. Cell Biol. 3 (11): 1020–4. doi:10.1038/ncb1101-1020. PMID 11715024.
Evellin S, Nolte J, Tysack K, et al. (2002). "Stimulation of phospholipase C-epsilon by the M3 muscarinic acetylcholine receptor mediated by cyclic AMP and the GTPase Rap2B.". J. Biol. Chem. 277 (19): 16805–13. doi:10.1074/jbc.M112024200. PMID 11877431.
Song C, Satoh T, Edamatsu H, et al. (2002). "Differential roles of Ras and Rap1 in growth factor-dependent activation of phospholipase C epsilon.". Oncogene 21 (53): 8105–13. doi:10.1038/sj.onc.1206003. PMID 12444546.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Bennasser Y, Badou A, Tkaczuk J, Bahraoui E (2003). "Signaling pathways triggered by HIV-1 Tat in human monocytes to induce TNF-alpha.". Virology 303 (1): 174–80. doi:10.1006/viro.2002.1676. PMID 12482669.
Czyzyk J, Brogdon JL, Badou A, et al. (2003). "Activation of CD4 T cells by Raf-independent effectors of Ras.". Proc. Natl. Acad. Sci. U.S.A. 100 (10): 6003–8. doi:10.1073/pnas.1031494100. PMC 156316. PMID 12721365. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=156316.
Lennartsson J, Wernstedt C, Engström U, et al. (2003). "Identification of Tyr900 in the kinase domain of c-Kit as a Src-dependent phosphorylation site mediating interaction with c-Crk.". Exp. Cell Res. 288 (1): 110–8. doi:10.1016/S0014-4827(03)00206-4. PMID 12878163.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
vom Dorp F, Sari AY, Sanders H, et al. (2005). "Inhibition of phospholipase C-epsilon by Gi-coupled receptors.". Cell. Signal. 16 (8): 921–8. doi:10.1016/j.cellsig.2004.01.009. PMID 15157671.

PDB gallery

2bye: NMR SOLUTION STRUCTURE OF PHOSPHOLIPASE C EPSILON RA 1 DOMAIN

2byf: NMR SOLUTION STRUCTURE OF PHOSPHOLIPASE C EPSILON RA 2 DOMAIN

2c5l: STRUCTURE OF PLC EPSILON RAS ASSOCIATION DOMAIN WITH HRAS

PLCE1

Function

References

Further reading