PIPES

PIPES
IUPAC name

1,4-Piperazinediethanesulfonic acid (IUPAC)
Other names

PIPES
Identifiers
CAS number 5625-37-6 Y
Properties
Molecular formula C8H18N2O6S2
Molar mass 302.37
Appearance White powder
Melting point

Decomposes above 300 °C
Boiling point

Decomposes
Solubility in water 1 g/L (100 °C)
Hazards
MSDS External MSDS
Main hazards Irritant
NFPA 704
0
1
0
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Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

PIPES is the common name for piperazine-N,N′-bis(2-ethanesulfonic acid), and frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s.[1]

Applications

PIPES has pKa (6.76 at 25°C) near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues.[2][3] Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer.[4] It has a negligible capacity to bind divalent ions.

References

  1. ^ Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. (1966). "Hydrogen Ion Buffers for Biological Research". Biochemistry 5 (2): 467–77. doi:10.1021/bi00866a011. PMID 5942950. 
  2. ^ Salema, R. and Brando, I., J. Submicr. Cytol., 9, 79 (1973).
  3. ^ Schiff, R.I. and Gennaro, J.F., Scaning Electron Microsc., 3, 449 (1979).
  4. ^ Hardham, A.R. (1985). "Studies on the cell surface of zoospores and cysts of the fungus Phytophthora cinnamomi: The influence of fixation on patterns of lectin binding". Journal of Histochemistry 33 (2): 110–8. doi:10.1177/33.2.3918095. PMID 3918095. http://www.jhc.org/cgi/content/abstract/33/2/110. 

See also