PIK3C2B

Phosphoinositide-3-kinase, class 2, beta polypeptide

Identifiers

PIK3C2B; C2-PI3K; DKFZp686G16234

External IDs

OMIM: 602838 MGI: 2685045 HomoloGene: 20582 GeneCards: PIK3C2B Gene

2.7.1.154

Gene Ontology
Molecular function	• nucleotide binding • lipid kinase activity • inositol or phosphatidylinositol kinase activity • protein binding • ATP binding • 1-phosphatidylinositol-3-kinase activity • phosphotransferase activity, alcohol group as acceptor • phosphatidylinositol-4-phosphate 3-kinase activity • phosphatidylinositol binding
Cellular component	• nucleus • cytoplasm • endoplasmic reticulum • microsome • cytosol • plasma membrane • phosphatidylinositol 3-kinase complex • endocytic vesicle
Biological process	• cell communication • biological_process • protein kinase B signaling cascade • phosphatidylinositol phosphorylation • phosphatidylinositol-mediated signaling
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

n/a

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 1:
204.39 – 204.46 Mb

Chr 1:
134.94 – 135.01 Mb

PubMed search

[1]

[2]

Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing beta polypeptide is an enzyme that in humans is encoded by the PIK3C2B gene.^[1]^[2]^[3]

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner.^[3]

References

^ Brown RA, Ho LK, Weber-Hall SJ, Shipley JM, Fry MJ (Jun 1997). "Identification and cDNA cloning of a novel mammalian C2 domain-containing phosphoinositide 3-kinase, HsC2-PI3K". Biochem Biophys Res Commun 233 (2): 537–44. doi:10.1006/bbrc.1997.6495. PMID 9144573.
^ Arcaro A, Volinia S, Zvelebil MJ, Stein R, Watton SJ, Layton MJ, Gout I, Ahmadi K, Downward J, Waterfield MD (Jan 1999). "Human phosphoinositide 3-kinase C2beta, the role of calcium and the C2 domain in enzyme activity". J Biol Chem 273 (49): 33082–90. doi:10.1074/jbc.273.49.33082. PMID 9830063.
^ ^a ^b "Entrez Gene: PIK3C2B phosphoinositide-3-kinase, class 2, beta polypeptide". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5287.

Lee C, Liu QH, Tomkowicz B, et al. (2004). "Macrophage activation through CCR5- and CXCR4-mediated gp120-elicited signaling pathways". J. Leukoc. Biol. 74 (5): 676–82. doi:10.1189/jlb.0503206. PMID 12960231.
Kozutsumi H, Toyoshima H, Hagiwara K, et al. (1994). "Human ltk receptor tyrosine kinase binds to PLC-gamma 1, PI3-K, GAP and Raf-1 in vivo". Oncogene 9 (10): 2991–8. PMID 8084603.
Milani D, Mazzoni M, Borgatti P, et al. (1996). "Extracellular human immunodeficiency virus type-1 Tat protein activates phosphatidylinositol 3-kinase in PC12 neuronal cells". J. Biol. Chem. 271 (38): 22961–4. doi:10.1074/jbc.271.38.22961. PMID 8798481.
Ueno H, Honda H, Nakamoto T, et al. (1997). "The phosphatidylinositol 3' kinase pathway is required for the survival signal of leukocyte tyrosine kinase". Oncogene 14 (25): 3067–72. doi:10.1038/sj.onc.1201153. PMID 9223670.
Mazerolles F, Barbat C, Fischer A (1997). "Down-regulation of LFA-1-mediated T cell adhesion induced by the HIV envelope glycoprotein gp160 requires phosphatidylinositol-3-kinase activity". Eur. J. Immunol. 27 (9): 2457–65. doi:10.1002/eji.1830270946. PMID 9341793.
Borgatti P, Zauli G, Colamussi ML, et al. (1998). "Extracellular HIV-1 Tat protein activates phosphatidylinositol 3- and Akt/PKB kinases in CD4+ T lymphoblastoid Jurkat cells". Eur. J. Immunol. 27 (11): 2805–11. doi:10.1002/eji.1830271110. PMID 9394803.
Borgatti P, Zauli G, Cantley LC, Capitani S (1998). "Extracellular HIV-1 Tat protein induces a rapid and selective activation of protein kinase C (PKC)-alpha, and -epsilon and -zeta isoforms in PC12 cells". Biochem. Biophys. Res. Commun. 242 (2): 332–7. doi:10.1006/bbrc.1997.7877. PMID 9446795.
Milani D, Mazzoni M, Zauli G, et al. (1998). "HIV-1 Tat induces tyrosine phosphorylation of p125FAK and its association with phosphoinositide 3-kinase in PC12 cells". AIDS 12 (11): 1275–84. doi:10.1097/00002030-199811000-00008. PMID 9708406.
Jauliac S, Mazerolles F, Jabado N, et al. (1998). "Ligands of CD4 inhibit the association of phospholipase Cgamma1 with phosphoinositide 3 kinase in T cells: regulation of this association by the phosphoinositide 3 kinase activity". Eur. J. Immunol. 28 (10): 3183–91. doi:10.1002/(SICI)1521-4141(199810)28:10<3183::AID-IMMU3183>3.0.CO;2-A. PMID 9808187.
Arcaro A, Zvelebil MJ, Wallasch C, et al. (2000). "Class II phosphoinositide 3-kinases are downstream targets of activated polypeptide growth factor receptors". Mol. Cell. Biol. 20 (11): 3817–30. doi:10.1128/MCB.20.11.3817-3830.2000. PMC 85707. PMID 10805725. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=85707.
Park IW, Wang JF, Groopman JE (2001). "HIV-1 Tat promotes monocyte chemoattractant protein-1 secretion followed by transmigration of monocytes". Blood 97 (2): 352–8. doi:10.1182/blood.V97.2.352. PMID 11154208.
Zauli G, Milani D, Mirandola P, et al. (2001). "HIV-1 Tat protein down-regulates CREB transcription factor expression in PC12 neuronal cells through a phosphatidylinositol 3-kinase/AKT/cyclic nucleoside phosphodiesterase pathway". FASEB J. 15 (2): 483–91. doi:10.1096/fj.00-0354com. PMID 11156964.
Wheeler M, Domin J (2001). "Recruitment of the class II phosphoinositide 3-kinase C2beta to the epidermal growth factor receptor: role of Grb2". Mol. Cell. Biol. 21 (19): 6660–7. doi:10.1128/MCB.21.19.6660-6667.2001. PMC 99811. PMID 11533253. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=99811.
Deregibus MC, Cantaluppi V, Doublier S, et al. (2002). "HIV-1-Tat protein activates phosphatidylinositol 3-kinase/ AKT-dependent survival pathways in Kaposi's sarcoma cells". J. Biol. Chem. 277 (28): 25195–202. doi:10.1074/jbc.M200921200. PMID 11994280.
Cook JA, August A, Henderson AJ (2002). "Recruitment of phosphatidylinositol 3-kinase to CD28 inhibits HIV transcription by a Tat-dependent mechanism". J. Immunol. 169 (1): 254–60. PMID 12077252.
Arcaro A, Khanzada UK, Vanhaesebroeck B, et al. (2002). "Two distinct phosphoinositide 3-kinases mediate polypeptide growth factor-stimulated PKB activation". EMBO J. 21 (19): 5097–108. doi:10.1093/emboj/cdf512. PMC 129034. PMID 12356726. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=129034.
Visnjić D, Crljen V, Curić J, et al. (2002). "The activation of nuclear phosphoinositide 3-kinase C2beta in all-trans-retinoic acid-differentiated HL-60 cells". FEBS Lett. 529 (2–3): 268–74. doi:10.1016/S0014-5793(02)03357-4. PMID 12372612.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.

PIK3C2B

References

Further reading