PIGT

Phosphatidylinositol glycan anchor biosynthesis, class T

Identifiers

PIGT; FLJ41596; MGC8909; NDAP

External IDs

OMIM: 610272 MGI: 1926178 HomoloGene: 6134 GeneCards: PIGT Gene

Gene Ontology
Molecular function	• GPI-anchor transamidase activity • protein binding
Cellular component	• cytoplasm • endoplasmic reticulum • endoplasmic reticulum membrane • membrane • integral to membrane • cytoplasmic membrane-bounded vesicle • integral to endoplasmic reticulum membrane • GPI-anchor transamidase complex
Biological process	• C-terminal protein lipidation • attachment of GPI anchor to protein • attachment of GPI anchor to protein • neuron differentiation • post-translational protein modification • cellular protein metabolic process • neuron apoptosis
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 20:
44.04 – 44.05 Mb

Chr 2:
164.32 – 164.33 Mb

PubMed search

[1]

[2]

GPI transamidase component PIG-T is an enzyme that in humans is encoded by the PIGT gene.^[1]^[2]^[3]

This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.^[2]

Interactions

PIGT has been shown to interact with PIGK^[4] and GPAA1.^[3]^[5]

References

^ Vainauskas S, Menon AK (Apr 2005). "Endoplasmic reticulum localization of Gaa1 and PIG-T, subunits of the glycosylphosphatidylinositol transamidase complex". J Biol Chem 280 (16): 16402–9. doi:10.1074/jbc.M414253200. PMID 15713669.
^ ^a ^b "Entrez Gene: PIGT phosphatidylinositol glycan anchor biosynthesis, class T". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=51604.
^ ^a ^b Ohishi K, Inoue N, Kinoshita T (August 2001). "PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8". EMBO J. 20 (15): 4088–98. doi:10.1093/emboj/20.15.4088. PMC 149153. PMID 11483512. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=149153.
^ Ohishi, Kazuhito; Nagamune Kisaburo, Maeda Yusuke, Kinoshita Taroh (Apr. 2003). "Two subunits of glycosylphosphatidylinositol transamidase, GPI8 and PIG-T, form a functionally important intermolecular disulfide bridge". J. Biol. Chem. (United States) 278 (16): 13959–67. doi:10.1074/jbc.M300586200. ISSN 0021-9258. PMID 12582175.
^ Vainauskas, Saulius; Maeda Yusuke, Kurniawan Henry, Kinoshita Taroh, Menon Anant K (Aug. 2002). "Structural requirements for the recruitment of Gaa1 into a functional glycosylphosphatidylinositol transamidase complex". J. Biol. Chem. (United States) 277 (34): 30535–42. doi:10.1074/jbc.M205402200. ISSN 0021-9258. PMID 12052837.

Eisenhaber B, Maurer-Stroh S, Novatchkova M, et al. (2003). "Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post-translational transfer to proteins.". Bioessays 25 (4): 367–85. doi:10.1002/bies.10254. PMID 12655644.
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Ohishi K, Inoue N, Kinoshita T (2001). "PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8". EMBO J. 20 (15): 4088–98. doi:10.1093/emboj/20.15.4088. PMC 149153. PMID 11483512. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=149153.
Yu Y, Zhang C, Zhou G, et al. (2001). "Gene expression profiling in human fetal liver and identification of tissue- and developmental-stage-specific genes through compiled expression profiles and efficient cloning of full-length cDNAs". Genome Res. 11 (8): 1392–403. doi:10.1101/gr.175501. PMC 311073. PMID 11483580. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=311073.
Fossey SC, Mychaleckyj JC, Pendleton JK, et al. (2001). "A high-resolution 6.0-megabase transcript map of the type 2 diabetes susceptibility region on human chromosome 20". Genomics 76 (1–3): 45–57. doi:10.1006/geno.2001.6584. PMID 11549316.
Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20". Nature 414 (6866): 865–71. doi:10.1038/414865a. PMID 11780052.
Vainauskas S, Maeda Y, Kurniawan H, et al. (2002). "Structural requirements for the recruitment of Gaa1 into a functional glycosylphosphatidylinositol transamidase complex". J. Biol. Chem. 277 (34): 30535–42. doi:10.1074/jbc.M205402200. PMID 12052837.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Ohishi K, Nagamune K, Maeda Y, Kinoshita T (2003). "Two subunits of glycosylphosphatidylinositol transamidase, GPI8 and PIG-T, form a functionally important intermolecular disulfide bridge". J. Biol. Chem. 278 (16): 13959–67. doi:10.1074/jbc.M300586200. PMID 12582175.
Hong Y, Ohishi K, Kang JY, et al. (2004). "Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins". Mol. Biol. Cell 14 (5): 1780–9. doi:10.1091/mbc.E02-12-0794. PMC 165076. PMID 12802054. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=165076.
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Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
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Li HL, Li Z, Qin LY, et al. (2006). "The novel neurotrophin-regulated neuronal development-associated protein, NDAP, mediates apoptosis". FEBS Lett. 580 (7): 1723–8. doi:10.1016/j.febslet.2006.02.022. PMID 16516892.

PIGT

Interactions

References

Further reading