ORC2L

Origin recognition complex, subunit 2
Identifiers
Symbols ORC2; ORC2
External IDs OMIM: 601182 MGI: 1328306 HomoloGene: 4512 GeneCards: ORC2 Gene
RNA expression pattern
More reference expression data
Orthologs
Species Human Mouse
Entrez 4999 18393
Ensembl ENSG00000115942 ENSMUSG00000026037
UniProt Q13416 Q543F8
RefSeq (mRNA) NM_006190.4 NM_001025378
RefSeq (protein) NP_006181 NP_001020549
Location (UCSC) Chr 2:
201.77 – 201.83 Mb		 Chr 1:
58.52 – 58.56 Mb
PubMed search [1] [2]

Origin recognition complex subunit 2 is a protein that in humans is encoded by the ORC2L gene.[1][2]

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3L, -4L, and -5L. It also interacts with CDC45L and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication.[2]

Interactions

ORC2L has been shown to interact with ORC1L,[3][4][5][6] Replication protein A1,[3] ORC4L,[3][7][8][5] ORC5L,[3][9][7][5] ORC3L,[3][7][10][11][5] MCM4,[3] CDC6,[3][12] MCM5,[3] MCM6,[3] MCM7,[3] MCM10,[13] DBF4,[3] MCM2[3] and ORC6L.[3][5]

References

  1. ^ Takahara K, Bong M, Brevard R, Eddy RL, Haley LL, Sait SJ, Shows TB, Hoffman GG, Greenspan DS (Oct 1996). "Mouse and human homologues of the yeast origin of replication recognition complex subunit ORC2 and chromosomal localization of the cognate human gene ORC2L". Genomics 31 (1): 119–122. doi:10.1006/geno.1996.0018. PMID 8808289. 
  2. ^ a b "Entrez Gene: ORC2L origin recognition complex, subunit 2-like (yeast)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4999. 
  3. ^ a b c d e f g h i j k l m Kneissl, Margot; Pütter Vera, Szalay Aladar A, Grummt Friedrich (Mar. 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. (England) 327 (1): 111–128. doi:10.1016/S0022-2836(03)00079-2. ISSN 0022-2836. PMID 12614612. 
  4. ^ Fujita, Masatoshi; Ishimi Yukio, Nakamura Hiromu, Kiyono Tohru, Tsurumi Tatsuya (Mar. 2002). "Nuclear organization of DNA replication initiation proteins in mammalian cells". J. Biol. Chem. (United States) 277 (12): 10354–10361. doi:10.1074/jbc.M111398200. ISSN 0021-9258. PMID 11779870. 
  5. ^ a b c d e Vashee, S; Simancek P, Challberg M D, Kelly T J (Jul. 2001). "Assembly of the human origin recognition complex". J. Biol. Chem. (United States) 276 (28): 26666–26673. doi:10.1074/jbc.M102493200. ISSN 0021-9258. PMID 11323433. 
  6. ^ Méndez, Juan; Zou-Yang X Helena, Kim So-Young, Hidaka Masumi, Tansey William P, Stillman Bruce (Mar. 2002). "Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication". Mol. Cell (United States) 9 (3): 481–491. doi:10.1016/S1097-2765(02)00467-7. ISSN 1097-2765. PMID 11931757. 
  7. ^ a b c Dhar, S K; Delmolino L, Dutta A (Aug. 2001). "Architecture of the human origin recognition complex". J. Biol. Chem. (United States) 276 (31): 29067–29071. doi:10.1074/jbc.M103078200. ISSN 0021-9258. PMID 11395502. 
  8. ^ Quintana, D G; Hou Zh, Thome K C, Hendricks M, Saha P, Dutta A (Nov. 1997). "Identification of HsORC4, a member of the human origin of replication recognition complex". J. Biol. Chem. (UNITED STATES) 272 (45): 28247–28251. doi:10.1074/jbc.272.45.28247. ISSN 0021-9258. PMID 9353276. 
  9. ^ Quintana, D G; Thome K C, Hou Z H, Ligon A H, Morton C C, Dutta A (Oct. 1998). "ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases". J. Biol. Chem. (UNITED STATES) 273 (42): 27137–27145. doi:10.1074/jbc.273.42.27137. ISSN 0021-9258. PMID 9765232. 
  10. ^ Matsuoka, Shuhei; Ballif Bryan A, Smogorzewska Agata, McDonald E Robert, Hurov Kristen E, Luo Ji, Bakalarski Corey E, Zhao Zhenming, Solimini Nicole, Lerenthal Yaniv, Shiloh Yosef, Gygi Steven P, Elledge Stephen J (May. 2007). "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage". Science (United States) 316 (5828): 1160–1166. doi:10.1126/science.1140321. PMID 17525332. 
  11. ^ Pinto, S; Quintana D G, Smith P, Mihalek R M, Hou Z H, Boynton S, Jones C J, Hendricks M, Velinzon K, Wohlschlegel J A, Austin R J, Lane W S, Tully T, Dutta A (May. 1999). "latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant". Neuron (UNITED STATES) 23 (1): 45–54. doi:10.1016/S0896-6273(00)80752-7. ISSN 0896-6273. PMID 10402192. 
  12. ^ Méndez, J; Stillman B (Nov. 2000). "Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis". Mol. Cell. Biol. (UNITED STATES) 20 (22): 8602–8612. doi:10.1128/MCB.20.22.8602-8612.2000. ISSN 0270-7306. PMC 102165. PMID 11046155. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=102165. 
  13. ^ Izumi, M; Yanagi K, Mizuno T, Yokoi M, Kawasaki Y, Moon K Y, Hurwitz J, Yatagai F, Hanaoka F (Dec. 2000). "The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase". Nucleic Acids Res. (ENGLAND) 28 (23): 4769–4777. doi:10.1093/nar/28.23.4769. PMC 115166. PMID 11095689. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=115166. 

Further reading